Structural biology of secondary active transporters
Project/Area Number |
22687007
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|
Keywords | X 線結晶構造解析 / 膜蛋白質 / 膜輸送体 / ドラッグデリバリー / 多剤排出因子 / X線結晶構造解析 |
Research Abstract |
Active transporters are membrane protein that mediates translocation of various substrates across the biological membrane by consuming energy, and thus are important for maintaining intra- and extracellular environment. The secondary active transporters translocate the small molecules, including amino acids, nucleic acids, sugars and drags,by utilizing the electrochemical gradient of H+ or Na+ across the membrane. In this project, we focused on the three important secondary active transporters, multidrug efflux transporter MATE, Ca2+/cation exchanger CaCA, and oligopeptide symporter POT, and performed structural and functional analyses of these targets.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger.2013
Author(s)
Nishizawa T, Kita S, Maturana AD,Furuya N, Hirata K, Kasuya G, Ogasawara S,Dohmae N, Iwamoto T, Ishitani R, NurekiO.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.2013
Author(s)
Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Iwase N, Tsukazaki T, Hattori M, Sugita Y, Kandori H, Newstead S, Ishitani R ,Nureki O.
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Journal Title
Proc Natl Acad Sci USA
Volume: (掲載決定)
Related Report
Peer Reviewed
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[Journal Article] Structural basis for the drug extrusion mechanism by a MATE multidrug transporter.2013
Author(s)
Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, Suga H, Nureki O.
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Journal Title
Nature
Volume: 496
Pages: 247-251
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of Cex1p reveals the mechanism of tRNA trafficking between nucleus and cytoplasm2013
Author(s)
Nozawa K, Ishitani R, Yoshihisa T, Sato M, Arisaka F, Kanamaru S, Dohmae N, Mangroo D, Senger B, Becker HD, Nureki O
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Journal Title
Nucleic Acids Res
Volume: 41
Pages: 3901
Related Report
Peer Reviewed
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[Journal Article] Structure and function of Zucchini endoribonuclease in piRNA biogenesis.2012
Author(s)
Nishimasu H, Ishizu H, Saito K, Fukuhara S, Kamatani MK, Bonnefond L, Matsumoto N, Nishizawa T, Nakanaga K, Aoki J, Ishitani R, Siomi H, Siomi MC, Nureki O.
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Journal Title
Nature
Volume: 491
Pages: 284-287
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the channelrhodopsin light-gated cation channel.2012
Author(s)
Kato HE, Zhang F, Yizhar O, RamakrishnanC, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O.
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Journal Title
Nature
Volume: 482
Pages: 369-374
Related Report
Peer Reviewed
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[Journal Article] Structure and function of Zucchini endoribonuclease in piRNA biogenesis2012
Author(s)
Nishimasu H, Ishizu H, Saito K, Fukuhara S, Kamatani MK, Bonnefond L, Matsumoto N, Nishizawa T, Nakanaga K, Aoki J, Ishitani R, Siomi H, Siomi MC, Nureki O
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Journal Title
Nature
Volume: 491
Pages: 284
DOI
Related Report
Peer Reviewed
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[Journal Article] Simplification of the genetic code: restricted diversity of genetically encoded amino acids2012
Author(s)
Kawahara-Kobayashi A, Masuda A, Araiso Y, Sakai Y, Kohda A, Uchiyama M, Asami S, Matsuda T, Ishitani R, Dohmae N, Yokoyama S, Kigawa T, Nureki O, Kiga D
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Journal Title
Nucleic Acids Res
Volume: 40
Pages: 10576
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure of a dominant-negative helix-loop-helix transcriptional regulator suggests mechanisms of autoinhibition2012
Author(s)
Ishii R, Isogaya K, Seto A, Koinuma D, Watanabe Y, Arisaka F, Yaguchi SI, Ikushima H, Dohmae N, Miyazono K, Miyazawa K, Ishitani R, Nureki O
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Journal Title
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the channelrhodopsin light-gated cation channel2012
Author(s)
Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O
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Journal Title
Nature
Volume: 482
Pages: 369-374
DOI
Related Report
Peer Reviewed
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