Structural basis for the epigenetic regulation by UHRF1 interacting proteins.研究代表者有田恭平(Kyohei Arita)

• Project/Area Number: 22687008
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: ARITA Kyohei 京都大学, 工学研究科, 助教 (40549648)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 構造生物学 / X線結晶構造解析 / エピジェネティクス / タンパク質相互作用 / タンパク質間相互作用 / UHRF1

Research Abstract

In this proposal, we focused UHRF1/2 proteins, which are key molecules of epigenetics regulation. To obtain the fundamental role of epigenetics, we performed the structural and biochemical study of UHRF1 interacting proteins and UHRF2. We revealed the function of UHRF1 interacting proteins and the mechanism of complex formation, and also showed the DNA binding property of UHRF2.

Research Products

• [Journal Article] Crystal structure of the ubiquitin-associated(UBA) domain of p62 and its interaction with ubiquitin (2011)
  • Author(s): Isogai S, Morimoto D, Arita K, Unzai S, Tenno T, Hasegawa J, Sou YS, Komatsu M, Tanaka K, Shirakawa M, Tochio H
  • Journal Title: Journal of Biochemistry Volume: 286 Pages: 31864-3187
  • Peer Reviewed
• [Journal Article] Crystal structure of the UBA domain of p62 and its interaction with ubiquitin (2011)
  • Author(s): Isogai, S., Morimoto, D., Arita, K., Unzai, S., Tenno, T., Hasegawa, J., Sou, Y.S., Komatsu, M., Tanaka, K., Shirakawa, M., Tochio, H.
  • Journal Title: J Biol Chem. Volume: 286 Issue: 36 Pages: 31864-318674
  • DOI: 10.1074/jbc.m111.259630
  • Peer Reviewed
• [Journal Article] Structural basis for recognition of poly-SUMO chain by a SUMO-like domain of Nip45 (2010)
  • Author(s): Sekiyama N, Arita K, Ikeda Y, Hashiguchi K, Ariyoshi M, Tochio H, Saitoh H, Shirakawa M
  • Journal Title: Proteins Volume: 78 Pages: 1491-1502
  • Peer Reviewed
• [Presentation] Structural basis for maintenance of DNA methylation and recognition of histone modifications by UHRF1 (2011)
  • Author(s): 有田恭平(白川昌宏代理)
  • Organizer: 第34回日本分子生物学会
  • Year and Date: 2011-12-15
• [Presentation] Structural basis for recognition of epigenetic marks by UHRF1 (2011)
  • Author(s): Kyohei Arita, Kazya Sugita、Motoko Unoki、Ryuji Hamamoto、Shin Isogai、Mariko Ariyoshi、Hidehito Tochio、Masahiro Shirakawa.
  • Organizer: 第11回日本蛋白質科学会年会
  • Year and Date: 2011-06-07
• [Presentation] Structural basis for recognition of epigenetic marks by UHRF1 (2011)
  • Author(s): Kyohei Arita, Kazya Sugita、Motoko Unoki、Ryuji Hamamoto、Shin Isogai、Mariko Ariyoshi、Hidehito Tochio、Masahiro Shirakawa
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク
  • Year and Date: 2011-06-07
• [Presentation] Structural basis for recognition of histone modifications by UHRF1 (2011)
  • Author(s): Kyohei Arita.
  • Organizer: 構造エピゲノム研究会第3回ワークショップ
  • Year and Date: 2011-04-25
• [Presentation] Structural basis for recognition of histone modifications by UHRF1 (2011)
  • Author(s): 有田恭平
  • Organizer: 構造エピゲノム研究会第3回ワークショップ
  • Place of Presentation: 理研横浜研究所交流棟ホール
  • Year and Date: 2011-04-25
• [Presentation] Structural basis for recognition of epigenetic modifications by UHRF1 (2011)
  • Author(s): Kyohei Arita.
  • Organizer: 新学術領域「天然変性タンパク質の分子認識機構と機能発現」第1回国際シンポジウム
  • Year and Date: 2011-01-27
• [Presentation] Structural basis for recognition of epigenetic modifications by UHRF1 (2011)
  • Author(s): Kyohei Arita
  • Organizer: 新学術領域「天然変性タンパク質の分子認識機構と機能発現」第1回国際シンポジウム
  • Place of Presentation: はまぎんホール・ヴィアマーレ
  • Year and Date: 2011-01-27
• [Presentation] UHRF1によるエピジェネティックマークの読み取り機構の構造生物学的基盤 (2010)
  • Author(s): 有田恭平, 杉田和也, 鵜木元香, 浜本隆二, 有吉真理子, 杤尾豪人, 白川昌宏
  • Organizer: BMB2010
  • Year and Date: 2010-12-10
• [Presentation] UHRF1によるエピジェネティックマークの読み取り機構の構造生物学的基盤 (2010)
  • Author(s): 有田恭平, 杉田和也, 鵜木元香, 浜本隆二, 有吉真理子, 朽尾豪人, 白川昌宏
  • Organizer: BMB2010
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-10
• [Presentation] UHRF1タンパク質によるヘミメチル化DNAとヒストン修飾の認識機構 (2010)
  • Author(s): 有田恭平, 杉田和也, 有吉真理子, 鵜木元香, 杤尾豪人, 中村祐輔, 白川昌宏
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Year and Date: 2010-05-29
• [Presentation] UHRF1タンパク質によるヘミメチル化DNAとヒストン修飾の認識機構 (2010)
  • Author(s): 有田恭平, 杉田和也, 有吉真理子, 鵜木元香, 朽尾豪人, 中村祐輪, 白川昌宏
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール
  • Year and Date: 2010-05-29
• [Book] Non coding RNA in plants (2011)
  • Author(s): Arita K., Kanno T., Yoshikawa M., and Habu Y.
  • Publisher: Springer
• [Book] Cytosine Methylation to Histone Modification in Arabidopsis thaliana (2011)
  • Author(s): Arita K., Kanno T., Yoshikawa M., Habu Y
  • Total Pages: 13
  • Publisher: Non coding RNA in plants (Springer出版)
• [Remarks]
  • URL: http://www.moleng.kyoto-u.ac.jp/~moleng_01/