Characterization of deubiquitylating enzymes in yeast

• Project/Area Number: 22687009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: 公益財団法人東京都医学総合研究所 (2012); Tokyo Metropolitan Organization for Medical Research (2010-2011)
• Principal Investigator: YASUSHI Saeki 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (80462779)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2012: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 細胞内タンパク質分解 / ユビキチン / タンパク質分解 / 蛋白質分解 / プロテアソーム / 脱ユビキチン化酵素 / 酵母 / 微生物

Research Abstract

Protein ubiquitylation is an essential post-translational modification responsible for targeted protein degradation, cellular signaling activation, DNA damage response, and protein trafficking. Deubiquitylating enzymes (DUBs) functions as an eraser of the ubiquitin signals by removing ubiquitin(s) from the ubiquitylated proteins. Despite of their importance, biological significance of DUBs poorly understood. To test enzymatic properties of DUBs, we expressed and purified yeast 20 DUBs by a wheat germ expression system. Although most DUBs did not have chain-type specificities, we found that four DUBs can cleave a ubiquitin precursor Ubi4. Among the proteasomal DUBs, consistently with previous studies, Rpn11 is essential for degradation of the ubiquitinated substrate whereas Ubp6 has an inhibitory effect for the substrate degradation in vitro. However, both DUBs can cleave both K48- and K63-linked ubiquitin chains, suggesting that other factors might specify the proteasomal degradation signal. We also found that Ubp6 facilitates proteasomal assembly by clearing ubiquitylated substrates from assembly precursors by its deubiquitylating activity. Furthermore, we applied parallel reaction monitoring (PRM) method to quantify the ubiquitin chains and analyzed DUB mutants.

Research Products

• [Journal Article] The parallel reaction monitoring method contributes to a highly sensitive polyubiquitin chain quantification (2013)
  • Author(s): Tsuchiya, H., Tanaka, K., and Saeki, Y
  • Journal Title: Biochem. Biophys. Res. Commun Volume: (in press)
  • Peer Reviewed
• [Journal Article] The parallel reaction monitoring method contributes to a highly sensitive polyubiquitin chain quantification. (2013)
  • Author(s): Tsuchiya H
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: XXX
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedi-cated chaperone Hsm3p (2012)
  • Author(s): Takagi, K., Kim, S., Yukii, H., Ueno, M., Morishita, R., Endo, Y., Kato, K., Tanaka, K., Saeki, Y. *, and Mizushima, T *, *Correspondences.
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 12172-12182
  • Peer Reviewed
• [Journal Article] Proteasomal degradation resolves competition between cell polarization and cellular wound healing (2012)
  • Author(s): Kono, K., Saeki, Y., Yoshida, S., Tanaka, K., Pellman, D
  • Journal Title: Cell Volume: 150 Pages: 151-164
  • Peer Reviewed
• [Journal Article] Hyrtioreticulins A-E, indole alkaloids inhibiting the ubiquitin-activating enzyme, from the marine sponge Hyrtios reticulatus (2012)
  • Author(s): Yamanokuchi, R., Imada, K., Miyazaki, M., Kato, H., Watanabe, T., Fujimuro, M., Saeki, Y., Yoshinaga, S., Terasawa, H., Iwasaki, N., Rotinsulu, H., Losung, F., Mangindaan, R.E., Namikoshi, M., de Voogd, N.J., Yokosawa, H., Tsukamoto, S
  • Journal Title: Bioorg. Med. Chem Volume: 20 Pages: 4437-4442
  • Peer Reviewed
• [Journal Article] New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 A resolution. Acta. Crystallogr (2012)
  • Author(s): Kim, S., Nishide, A., Saeki, Y., Takagi, K., Tanaka, K., Kato, K., Mizushima, T
  • Journal Title: Sect. F. Struct. Biol. Cryst. Commun Volume: 68 Pages: 517-521
  • Peer Reviewed
• [Journal Article] Insulin/insulin-like growth factor (IGF) stimulation abrogates an association between a deubiquitinating enzyme USP7 and insulin receptor substrates (IRSs) followed by proteasomal degradation of IRSs (2012)
  • Author(s): Yoshihara, H., Fukushima T, Hakuno, F., Saeki, Y., Tanaka, K., Ito, A., Yoshida, M., Iemura, S., Natsume, T., Asano, T., Chida, K., Girnita, L., Takahashi, S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 423 Pages: 122-127
  • Peer Reviewed
• [Journal Article] Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy (2012)
  • Author(s): Sakata, E., Bohn, S., Mihalache, O., Kiss, P., Beck, B., Nagy, I., Nickell, S., Tanaka, K., Saeki, Y., Forster, F., and Baumeister, W
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 1479-1484
  • Peer Reviewed
• [Journal Article] Insulin/insulin-like growth factor (IGF) stimulation abrogates an association between a deubiquitinating enzyme USP7 and insulin receptor substrates (IRSs) followed by proteasomal degradation of IRSs (2012)
  • Author(s): Yoshihara H, Fukushima T, Hakuno F, Saeki Y, Tanaka K, Ito A, Yoshida M, Iemura S, Natsume T, Asano T, Chida K, Girnita L, Takahashi S-I
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 423 Issue: 1 Pages: 122-127
  • DOI: 10.1016/j.bbrc.2012.05.093
  • Peer Reviewed
• [Journal Article] Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy. (2012)
  • Author(s): Sakata E
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 109 Issue: 5 Pages: 1479-1484
  • DOI: 10.1073/pnas.1119394109
  • Peer Reviewed
• [Journal Article] Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy (2012)
  • Author(s): Sakata, E., et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 1479-1484
  • Peer Reviewed
• [Journal Article] Structural basis for specific recognition of Rpt1p, an ATPase subunit of the 26S proteasome, by a proteasome-dedicated chaperone Hsm3p (2012)
  • Author(s): Takagi, T., et al
  • Journal Title: J Biol Chem Volume: 287 Pages: 12172-12182
  • Peer Reviewed
• [Journal Article] The proteasome : molecular machinery and pathophysiological roles (2012)
  • Author(s): Tanaka, K., et al
  • Journal Title: Biol Chem Volume: 393 Pages: 217-234
  • Peer Reviewed
• [Journal Article] The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle (2011)
  • Author(s): Sakata, E., Stengel, F., Fukunaga, K., Zhou, M., Saeki, Y., Forster, F., Baumeister, W., Tanaka, K., and Robinson, C.V
  • Journal Title: Mol Cell Volume: 42 Pages: 637-649
  • Peer Reviewed
• [Journal Article] Sharpin is a component of the NF-.B activating linear ubiquitin chain assembly complex (2011)
  • Author(s): Tokunaga, F., Nakagawa, T., Nakahara, M., Saeki, Y., Taniguchi, M., Sakata, S., Tanaka, K., Nakano, H., and Iwai, K
  • Journal Title: Nature Volume: 471 Pages: 633-636
  • Peer Reviewed
• [Journal Article] Sharpin is a component of the NF-κB activating linear ubiquitin chain assembly complex. (2011)
  • Author(s): Tokunaga, F., et al.
  • Journal Title: Nature Volume: 471 Pages: 633-636
  • Peer Reviewed
• [Journal Article] The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle. (2011)
  • Author(s): Sakata, E., et al.
  • Journal Title: Mol.Cell Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Polyubiquitin conjugation to NEMO by tripartite motif protein 23 (TRIM23) is critical in antiviral defence (2010)
  • Author(s): Arimoto, K.I., Funami, K., Saeki, Y., Tanaka, K., Okawa, K., Takeuchi, O., Akira, S., Murakami, Y., and Shimotohno, K
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 15856-15861
  • Peer Reviewed
• [Journal Article] Dissection of the assembly pathway of the proteasome lid in Saccharomyces cerevisiae (2010)
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 396 Pages: 1048-1053
  • Peer Reviewed
• [Journal Article] Crystal structure of yeast Rpn14, a chaperone of the 19S regulatory particle of the proteasome (2010)
  • Author(s): Kim, S. *, Saeki, Y. *, Fukunaga, K., Suzuki, A., Takagi, K., Yamane, T., Tanaka, K., Mizushima, T., and Kato, K, *Equal contribution
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 15159-15166
  • Peer Reviewed
• [Journal Article] Crystal structure of yeast Rpn14, a chaperone of the 19S regulatory particle of the proteasome. (2010)
  • Author(s): Kim, S., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 15159-15166
  • Peer Reviewed
• [Journal Article] Dissection of the assembly pathway of the proteasome lid in Saccharomyces cerevisiae. (2010)
  • Author(s): Fukunaga, K., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 1048-1053
  • Peer Reviewed
• [Journal Article] Polyubiquitin conjugation to NEMO by tripartite motif protein 23 (TRIM23) is critical in antiviral defense. (2010)
  • Author(s): Arimoto, KI., et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107 Pages: 15856-15861
  • Peer Reviewed
• [Presentation] プロテアソームダイナミクス (2013)
  • Author(s): 佐伯 泰
  • Organizer: 理化学研究所シンポジウム「プロテアソームダイナミクスの理解を目指して」
  • Place of Presentation: 東京
  • Year and Date: 2013-01-17
• [Presentation] Structure and dynamics of the 26S proteasome (2012)
  • Author(s): Yasushi Saeki
  • Organizer: 熊本大学発生医学研究所リエゾンラボ研究会
  • Place of Presentation: 熊本
  • Year and Date: 2012-12-19
• [Presentation] 新規ユビキチン鎖長決定法の開発 (2012)
  • Author(s): 土屋 光、海保 愛、田中啓二、佐伯 泰
  • Organizer: 第35回分子生物学会ワークショップ
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] 出芽酵母の休止期において形成されるプロテアソーム顆粒の解析 (2012)
  • Author(s): 雪井 悠、新井直子、田中啓二、佐伯 泰
  • Organizer: 第35回分子生物学会
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] プロテアソーム可塑性:分子集合から顆粒形成まで (2012)
  • Author(s): 佐伯 泰、雪井 悠、土屋 光、田中啓二
  • Organizer: 第85回日本生化学会大会シンポジウム
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] 選択的なポリユビキチン鎖の検出・定量法の開発 (2012)
  • Author(s): 佐伯 泰
  • Organizer: 平成24年度 文部科学省 新学術領域研究「ユビキチンネオバイオロジー:拡大するタンパク質制御システム」kick-off シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2012-09-26
• [Presentation] 新規ユビキチン鎖長決定法の開発 (2012)
  • Author(s): 土屋 光、海保 愛、田中啓二、佐伯 泰
  • Organizer: 酵母遺伝学フォーラム第45回研究報告会
  • Place of Presentation: 宇治
  • Year and Date: 2012-09-05
• [Presentation] 出芽酵母の休止期において形成されるプロテアソームストレージ顆粒の解析 (2012)
  • Author(s): 雪井 悠、新井直子、田中啓二、佐伯 泰
  • Organizer: 酵母遺伝学フォーラム第45回研究報告会
  • Place of Presentation: 宇治
  • Year and Date: 2012-09-05
• [Presentation] 出芽酵母26Sプロテアソームは細胞質で完成する (2012)
  • Author(s): 佐伯 泰、白 燦基、東江昭夫、田中啓二
  • Organizer: 酵母遺伝学フォーラム第45回研究報告会
  • Place of Presentation: 宇治
  • Year and Date: 2012-09-05
• [Presentation] Regulation by ubiquitin proteasome system (2012)
  • Author(s): Yasushi Saeki
  • Organizer: Core-to-Core Project sponsored by JSPS International Seminar, New Insights into the Molecular Basis of Prevention of Diseases in the Aging Society Caused by Modulation of Insulin-Like Activities
  • Place of Presentation: 東京
  • Year and Date: 2012-08-25
• [Presentation] 細胞内タンパク質分解装置プロテアソームの分子集合と作動機構 (2012)
  • Author(s): 佐伯 泰, 坂田絵理, 白 燦基、佐甲靖志, W. Baumeister,田中啓二
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-06-21
• [Presentation] 巨大で複雑なタンパク分解装置の動態と作動機構.細胞内タンパク質分解装置の構造と活性調節機構 (2011)
  • Author(s): 佐伯 泰, 西尾和也, 坂田絵理, 福永圭佑, 雪井 悠, W. Baumeister, 森本幸生, 田中啓二
  • Organizer: 平成23年度ターゲットタンパク研究プログラム成果発表会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-27
• [Presentation] 出芽酵母26Sプロテアソームは細胞質で完成する (2011)
  • Author(s): 佐伯 泰、白 燦基、東江昭夫、田中啓二
  • Organizer: 第44回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Wolfgang Baumeister, Keiji Tanaka:Ubp6 and Rad23 are involved in the 26S proteasome assembly (2011)
  • Author(s): Keisuke Fukunaga, Eri Sakata, Florian Stengel, Yasushi Saeki, Carol V. Robinson
  • Organizer: 第44回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] プロテアソーム研究の最前線 (2011)
  • Author(s): 佐伯 泰
  • Organizer: バイオコンファレンス2011首都大学東京
  • Place of Presentation: 東京
  • Year and Date: 2011-11-11
• [Presentation] (2011)
  • Author(s): 佐伯 泰
  • Organizer: 東京大学大学院農学生命科学科 第7回応用動物科学セミナー「プロテアソーム研究の最前線」
  • Place of Presentation: 東京
  • Year and Date: 2011-10-25
• [Presentation] Rad23とUbp6は26Sプロテアソームの分子集合に関与する (2011)
  • Author(s): 佐伯泰
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-10-25
• [Presentation] Rad23とUbp6は26Sプロテアソームの分子集合に関与する (2011)
  • Author(s): 佐伯 泰、福永圭佑、坂田絵里、Florian Stengel、Carol V. Robinson、Wolfgang Baumeister、田中啓二
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] Ubp6はプロテアソームの分子集合を正に制御する (2011)
  • Author(s): 福永圭佑、坂田絵里、佐伯 泰、田中啓二
  • Organizer: 酵母遺伝学フォーラム第44回研究報告会
  • Place of Presentation: 博多
  • Year and Date: 2011-09-07
• [Presentation] 出芽酵母26Sプロテアソームは細胞質で完成する (2011)
  • Author(s): 佐伯 泰、白 燦基、東江昭夫、田中啓二
  • Organizer: 酵母遺伝学フォーラム第44回研究報告会
  • Place of Presentation: 博多
  • Year and Date: 2011-09-07
• [Presentation] 26Sプロテアソームの分子集合と作動原理 (2011)
  • Author(s): 佐伯泰
  • Organizer: 第19回酵母合同シンポジウム
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-06-24
• [Presentation] 26Sプロテアソームの分子集合機構 (2011)
  • Author(s): 佐伯 泰, 福永圭佑, 工藤 泰, 川村ひとみ, 田中啓二
  • Organizer: 平成22年度ターゲットタンパク研究プログラム成果発表会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-11
• [Presentation] Assembly, structure, and function of the 26S proteasome. (2011)
  • Author(s): Yasushi Saeki
  • Organizer: Korea-Japan Symposium on Protein Metabolism
  • Place of Presentation: Seoul, Korea(招待講演)
  • Year and Date: 2011-03-11
• [Presentation] Ubp6 and Rad23 are involved in the 26S proteasome assembly (2011)
  • Author(s): Keisuke Fukunaga, Eri Sakata, Florian Stengel, Yasushi Saeki, Carol V. Robinson, Wolfgang Baumeister, Keiji Tanaka
  • Organizer: EMBO Conference, Ubiquitin and ubiquitin-like modifiers
  • Place of Presentation: Cavtat, Croatia
• [Presentation] The 26S proteasome completes its assembly process in the cytoplasm prior to the nuclear translocation (2011)
  • Author(s): Haruka Yukii, Yasushi Saeki, Changi Pack, Keisuke Fukunaga, Eri Sakata, Yasushi Sako, Akio Toh-e, Wolfgang Baumeister, Keiji Tanaka
  • Organizer: EMBO Conference, Ubiquitin and ubiquitin-like modifiers
  • Place of Presentation: Cavtat, Croatia
• [Presentation] Assembly, structure, and function of the 26S proteasome (2011)
  • Author(s): Yasushi Saeki
  • Organizer: Korea-Japan Symposium on Protein Metabolism
  • Place of Presentation: Seoul, Korea
• [Presentation] 26Sプロテアソームの分子集合と作動原理 (2010)
  • Author(s): 佐伯 泰
  • Organizer: 第19回酵母合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] 26Sプロテアソームはどこで完成するか? (2010)
  • Author(s): 佐伯 泰, 白燦基, 川村ひとみ, 東江昭夫, 佐甲靖志, 田中啓二
  • Organizer: BMB2010(第33回分子生物学会、第83回日本生化学会合同年会)
  • Place of Presentation: 神戸
• [Presentation] Assembly pathway of the 19S proteasome lid in yeast (2010)
  • Author(s): 福永圭佑, 工藤 泰, 東江昭夫, 田中啓二, 佐伯 泰
  • Organizer: BMB2010(第33回分子生物学会、第83回日本生化学会合同年会)
  • Place of Presentation: 神戸
• [Presentation] A proteomic analysis of ubiquitin-binding proteins by unclavable polyubiquitins in yeast (2010)
  • Author(s): 工藤 泰, 福永圭祐, 田中啓二, 佐伯 泰
  • Organizer: BMB2010(第33回分子生物学会、第83回日本生化学会合同年会)
  • Place of Presentation: 神戸
• [Presentation] プロテアソーム19S制御因子複合体の分子集合機構 (2010)
  • Author(s): 佐伯 泰, 福永圭佑, 田中啓二
  • Organizer: 平成22年度ターゲットタンパク研究プログラム成果発表会
  • Place of Presentation: 東京
• [Presentation] 出芽酵母19Sプロテアソーム制御因子Lid複合体の分子集合 (2010)
  • Author(s): 福永圭佑, 工藤 泰, 東江昭夫, 田中啓二, 佐伯 泰
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: 奈良
• [Presentation] A quantitative proteomic analysis of tUb-binding proteins with different length in yeast (2010)
  • Author(s): Keisuke Fukunaga, Yasushi Saeki, and Keiji Tanaka
  • Organizer: 1st conference on PPDUP
  • Place of Presentation: Vancouver, Canada
• [Presentation] 細胞内タンパク質分解装置プロテアソームの分子集合と作動機構
  • Author(s): 佐伯 泰
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] Regulation by ubiquitin-proteasome system
  • Author(s): Yasushi Saeki
  • Organizer: Core-to-Core Project sponsored by JSPS International Seminar,
  • Place of Presentation: 東京
  • Invited
• [Presentation] Structure and dynamics of the 26S proteasome.
  • Author(s): Yasushi Saeki
  • Organizer: 熊本大学発生医学研究所リエゾンラボ研究会
  • Place of Presentation: 熊本
  • Invited
• [Book] The proteasome: molecular machinery and pathophysiological roles. Biol. Chem. 393 (2012)
  • Author(s): Tanaka, K., Mizushima, N., and Saeki, Y
• [Book] Assembly and function of the proteasome. (Ubiquitin family modifiers and the proteasome) (2012)
  • Author(s): Saeki, Y. and Tanaka, K. Review
  • Publisher: Humana Press, Methods Mol. Biol. 832
• [Book] 巨大で複雑な蛋白分解装置の立体構造と活性調節機構.「特集/蛋白構造-機能連関解析と治療薬開発への応用」内分泌・糖尿病・代謝内科、第35巻12月号 (2012)
  • Author(s): 佐伯 泰
  • Publisher: 科学評論社
• [Book] 細胞膜修復機構におけるプロテアソームの役割.細胞工学31巻 (2012)
  • Author(s): 河野恵子,佐伯 泰,吉田知史,田中啓二,David Pellman
  • Publisher: 秀潤社
• [Book] 特集／蛋白構造－機能連関解析と治療薬開発への応用」内分泌・糖尿病・代謝内科 (2012)
  • Author(s): 佐伯 泰
  • Total Pages: 10
  • Publisher: 科学評論社
• [Book] Ubiquitin Family Modifiers and the Proteasome (2012)
  • Author(s): Saeki, Y., Tanaka, K.
  • Total Pages: 22
  • Publisher: Humana Press
• [Book] タンパク質分解研究による生態防御 (2012)
  • Author(s): 佐伯泰
  • Total Pages: 6
  • Publisher: 羊土社
• [Book] 脱ユビキチン化酵素Ubp6はプロテアソームの分子集合を制御する (2011)
  • Author(s): 佐伯 泰,田中啓二
  • Publisher: ライフサイエンス「新着論文レビュー」
• [Book] みえてきた26Sプロテアソームの形成機構の全貌,「細胞内分解系によるリノベーションの分子機構に迫る」実験医学・増刊号29巻(12号) (2011)
  • Author(s): 佐伯 泰
  • Publisher: 羊土社
• [Book] Methods in Molecular Biology (Ubiquitin family modifiers and the proteasome) (2011)
  • Author(s): Saeki, Y., Tanaka, K.
  • Publisher: Humana Press(印刷中)
• [Book] 実験医学・増刊号「細胞内分解系によるリノベーションの分子機構に迫る」 (2011)
  • Author(s): 佐伯泰、福永圭佑
  • Publisher: 羊土社(印刷中)
• [Book] プロテアソーム阻害剤. 日本臨牀, 68 (2010)
  • Author(s): 佐伯 泰, 福永圭佑, 田中啓二:
  • Publisher: 日本臨牀社
• [Book] 日本臨牀 特集 分子標的薬治療 (2010)
  • Author(s): 佐伯泰、福永圭佑、田中啓二
  • Total Pages: 5
  • Publisher: 日本臨牀社
• [Remarks]
  • URL: http://www.igakuken.or.jp/pro-meta/
• [Remarks] 公益財団法人東京都医学総合研究所蛋白質代謝研究室
  • URL: http://www.igakuken.or.jp/pro-meta/
• [Remarks]
  • URL: http://check.shonai/pro-meta/studies/saeki.html
• [Remarks]
  • URL: http://www.rinshoken.or.jp/MO/