A study of 3D mechanical network of the spindle-like structure composed of microtubules and kinesins.
| Project/Area Number |
22687012
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAJIMA Junichiro 東京大学, 大学院・総合文化研究科, 准教授 (00453499)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2010: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Keywords | 分子モーター / 細胞骨格 / 3 次元計測 / ３次元計測 / 力計測 / 分裂期キネシン / 3次元計測 / 紡錘体様構造 |
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| Research Abstract |
We have examined that the motility driven by (1) kinesins functioning in mitosis and (2)or axonemal dyneins being involved in ciliary movement. We found that kinesin-6 and outer arm dynein rotated sliding microtubules around their longitudinal axis. These corkscrewing motion of microtubule has not been observed in kinesin-6 or outer arm dynein from any source. The corkscrewing motion depends on ATP concentration in the buffer,phosphorylation on N-terminal region of kinesin-6 head, binding protein or direction of load. Our results suggested that torque generation of microtubule-based motors may be regulated by external conditions.
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Report
(4 results)
Research Products
(38 results)
