Analyses of regulatory mechanisms of chloroplast division by eukaryotic cell cycle and photosynthesis

• Project/Area Number: 22687020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: National Institute of Genetics (2011-2013); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: MIYAGISHIMA Shin-ya 国立遺伝学研究所, 大学共同利用機関等の部局等, その他 (00443036)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: オルガネラ起源 / 葉緑体 / 細胞内共生 / 進化 / 細胞周期 / 細胞分裂 / 分裂

Research Abstract

Chloroplasts have evolved from a cyanobacterial endosymbiont. Continuity of chloroplasts has been maintained by synchronization of the host cell cycle and the timing of chloroplast division. In this study, we have shown (1) that timing of chloroplast division is restricted to the S phase by the cell-cycle regulated expression of the chloroplast division genes and (2) that the host cell cycle progression depends on both photosynthetic cell growth and circadian rhythmus.

Research Products

• [Journal Article] Chloroplast DNA replication is regulated by the redox state independently of chloroplast division in Chlamydomonas reinhardtii (2013)
  • Author(s): Yukihiro Kabeya and Shin-ya Miyagishima
  • Journal Title: Plant Physiol. Volume: 161 Issue: 4 Pages: 2102-2112
  • DOI: 10.1104/pp.113.216291
  • Peer Reviewed
• [Journal Article] Expression of nucleus-encoded chloroplast division genes and proteins regulated by algai cell cycle.(掲載確定) (2012)
  • Author(s): Shin-ya Miyagishima, Kenji Suzuki, Kumiko Okazaki, Yukihiro Kabeya
  • Journal Title: Molecular Biology and Evolution Volume: 29 Issue: 10 Pages: 2957-2970
  • DOI: 10.1093/molbev/mss102
  • Peer Reviewed
• [Journal Article] Plastid-dividing machinery: Formation, Contraction and fission. (2012)
  • Author(s): Yoshida Y., et al.
  • Journal Title: Curr Opi Plant Biol Volume: 15 Issue: 6 Pages: 714-721
  • DOI: 10.1016/j.pbi.2012.07.002
  • Peer Reviewed
• [Journal Article] Expression of the nucleus-encoded chloroplast division genes and proteins regulated by the algal cell cycle (2012)
  • Author(s): Miyagishima, S., et al
  • Journal Title: Mol.Biol.Evol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Structure, regulation, and evolution of the plastid division machinery (2011)
  • Author(s): Shin-ya Miyagishima, Hiromitsu Nakanishi, and Yukihiro Kabeya
  • Journal Title: Int. Rev. Cell. Mol. Biol. Volume: 291 Pages: 115-153
  • DOI: 10.1016/b978-0-12-386035-4.00004-5
  • ISBN: 9780123860354
  • Peer Reviewed
• [Journal Article] Evolution and regulation of the plastid division machinery (2011)
  • Author(s): Miyagishima, S.
  • Journal Title: Plant Physiol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Chloroplast division: squeezing thephotosynthetic captive (2010)
  • Author(s): Miyagishima, SY. & Kabeya, Y
  • Journal Title: Curr. Opin.Microbiol Volume: 13 Issue: 4 Pages: 738-746
  • DOI: 10.1104/pp.110.170688
  • Peer Reviewed
• [Journal Article] Chloroplast division : squeezing the photosynthetic captive (2010)
  • Author(s): Shin-ya Miyagishima and Yukihiro Kabeya
  • Journal Title: Curr. Opin. Microbiol. Volume: 13 Issue: 6 Pages: 738-746
  • DOI: 10.1016/j.mib.2010.10.004
  • Peer Reviewed
• [Journal Article] Nucleus-independent control of the rubiscooperon by the plastid-encodedtranscription factor Ycf30 in the red algaCyanidioschyzon merolae (2010)
  • Author(s): Minoda, A., Weber, A.P., Tanaka, K., &Miyagishima, SY
  • Journal Title: Plant Physiol. Volume: 154 Issue: 3 Pages: 1532-1540
  • DOI: 10.1104/pp.110.163188
  • Peer Reviewed
• [Journal Article] The YlmG protein has aconserved function related to thedistribution of nucleoids in chloroplasts andcyanobacteria (2010)
  • Author(s): Kabeya, Y. et al. Miyagishima, SY.
  • Journal Title: BMC Plant Biol. Volume: 10 Issue: 1 Pages: 57-57
  • DOI: 10.1186/1471-2229-10-57
  • Peer Reviewed
• [Journal Article] Eukaryotic and eubacterial contributions tothe establishment of plastid proteomeestimated by large-scale phylogeneticanalyses (2010)
  • Author(s): Suzuki K. & Miyagishima, SY
  • Journal Title: Mol. Biol. Evol Volume: 27 Issue: 3 Pages: 581-590
  • DOI: 10.1093/molbev/msp273
  • Peer Reviewed
• [Journal Article] The evolution of the regulatorymechanism of chloroplast division (2010)
  • Author(s): Okazaki, K., Kabeya, Y. & Miyagishima, SY
  • Journal Title: PlantSignal Behav Volume: 5 Issue: 2 Pages: 164-167
  • DOI: 10.4161/psb.5.2.10461
  • Peer Reviewed
• [Journal Article] Chloroplast division : squeezing the photosynthetic captive. (2010)
  • Author(s): Miyagishima, S.
  • Journal Title: Curr.Opin.Microbiol. Volume: 13 Pages: 738-746
  • Peer Reviewed
• [Journal Article] Nucleus-independent control of the Rubisco operon by the plastid-encoded transcription factor Ycf30 in the red alga Cyanidioschyzon merolae. (2010)
  • Author(s): Minoda, A.
  • Journal Title: Plant Physiol. Volume: 154 Pages: 1532-1540
  • Peer Reviewed
• [Presentation] The coordinating mechanisms of eukaryotic cell and chloroplast proliferation (2012)
  • Author(s): 宮城島 進也
  • Organizer: 日本微生物生態学会大会シンポジウム
  • Place of Presentation: 豊橋市
  • Year and Date: 2012-09-22
• [Presentation] オルガネラ分裂から見た細胞内共生と植物細胞の進化 (2012)
  • Author(s): 宮城島 進也
  • Organizer: 日本植物学会大会シンポジウム
  • Place of Presentation: 姫路市
  • Year and Date: 2012-09-16
• [Presentation] 細胞と葉緑体の分裂同調化機構の解析 (2011)
  • Author(s): 宮城島 進也
  • Organizer: クラミドモナス・ワークショップ
  • Place of Presentation: 岡崎市
  • Year and Date: 2011-11-26
• [Presentation] 細胞と葉緑体の分裂同調化機構の解析 (2011)
  • Author(s): 宮城島進也
  • Organizer: 第9回クラミドモナス・ワークショップ
  • Place of Presentation: 基礎生物学研究所(岡崎市)(招待講演)
  • Year and Date: 2011-11-26
• [Presentation] 細胞内共生 : ミトコンドリアと葉緑体 (2011)
  • Author(s): 宮城島 進也
  • Organizer: 地文台シンポジウム&東工大流動機構国際ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2011-11-01
• [Presentation] 細胞内共生:ミトコンドリアと葉緑体 (2011)
  • Author(s): 宮城島進也
  • Organizer: 第6回地文台シンポジウム&東工大流動機構国際ワークショップ
  • Place of Presentation: 東京工業大学(東京都目黒区)(招待講演)
  • Year and Date: 2011-11-01
• [Presentation] Evolution and Regulation of the Chloroplast Division Machinery : Permanent Inheritance of Endosymbiotic Organelles (2011)
  • Author(s): 宮城島 進也
  • Organizer: 日本生化学会大会シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-23
• [Presentation] Evolution and Regulation of the Chloroplast Division Machinery : Permanent Inheritance of Endosymbiotic Organelles (2011)
  • Author(s): 宮城島進也
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都市)(招待講演)
  • Year and Date: 2011-09-23
• [Presentation] 藻類細胞周期による葉緑体分裂制御機構 (2011)
  • Author(s): 宮城島 進也、壁谷 如洋
  • Organizer: 日本植物学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-17
• [Presentation] 藻類細胞周期による葉緑体分裂制御機構 (2011)
  • Author(s): 宮城島進也
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京都目黒区)
  • Year and Date: 2011-09-17
• [Presentation] 葉緑体の分裂機構と分裂制御機構 (2010)
  • Author(s): 宮城島 進也
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] 細胞と葉緑体の分裂同調化機構の解析 (2010)
  • Author(s): 宮城島 進也
  • Organizer: 日本植物学会大会
  • Place of Presentation: 春日井市
  • Year and Date: 2010-09-10
• [Presentation] 細胞と葉緑体の分裂同調化機構の解析 (2010)
  • Author(s): 宮城島進也
  • Organizer: 日本植物学会
  • Place of Presentation: 春日井市
  • Year and Date: 2010-09-10
• [Presentation] 真核細胞と葉緑体の協調増殖機構 (2010)
  • Author(s): 宮城島 進也
  • Organizer: 日本植物細胞分子生物学会シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-02
• [Presentation] 真核細胞と葉緑体の協調増殖機構 (2010)
  • Author(s): 宮城島進也
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-02
• [Presentation] The coordinating mechanisms of eukaryotic cell and chloroplast proliferation
  • Author(s): Miyagishima, S.
  • Organizer: 第28回日本微生物生態学会大会シンポジウム
  • Place of Presentation: 豊橋市
  • Invited
• [Presentation] オルガネラ分裂から見た細胞内共生と植物細胞の進化
  • Author(s): 宮城島 進也
  • Organizer: 日本植物学会第76回大会シンポジウム
  • Place of Presentation: 姫路市
  • Invited
• [Book] 葉緑体の分裂機構, 細胞工学, 30巻11号 (2011)
  • Author(s): 宮城島 進也、壁谷 如洋
  • URL: http://gakken-mesh.jp/journal/detail/9784780901245.html
• [Book] Chloroplast division machinery : origins and evolutions. In Red Algae in Genomic Age. (eds. J. Sechbach, D.J. Chapman, and A. Weber) (2010)
  • Author(s): Shin-ya Miyagishima and Hiromitsu Nakanishi
  • Publisher: Springer(DOI:10.1007/978-90-481-3795-4)
• [Remarks]
  • URL: http://www.nig.ac.jp/section/miyagisima/miyagisima-j.html
• [Remarks]
  • URL: http://www.nig.ac.jp/section/miyagisima/miyagisima-j.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/iru/miyagishima/index.html