Improvement of the membrane permeability of compounds used by streptothricin biosynthetic enzymes
| Project/Area Number |
22688007
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | NRPS / ペプチド合成酵素 / 抗生物質 / 生合成 / 放線菌 / 二次代謝産物 / ストレプトスリシン / 生合成遺伝子 / 2次代謝産物 / 酵素 |
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| Research Abstract |
The streptothricin (ST) antibiotics, produced by Streptomyces bacteria, contain L-β-lysine oligopeptides as pendant chains. In this study, we identified three unusual nonribosomal peptide synthetases (NRPSs) involved in ST biosynthesis: ORF 5 (a stand-alone adenylation (A) domain), ORF 18 (containing thiolation (T) and condensation (C) domains) and ORF 19 (a stand-alone A domain).
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] A stand-alone adenylation domain forms amide bonds in streptothricin biosynthesis2012
Author(s)
Chitose Maruyama, Junya Toyoda, YasYasuo Kato, Miho Izumikawa, Motoki TakYasuo Kato, Miho Izumikawa, Motoki Takagi, Kazuo Shin-ya, Hajime Katano, Takashi Utagawa, and Yoshimitsu Hamano
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Journal Title
Nature Chem. Biol
Volume: 8
Issue: 9
Pages: 791-797
DOI
Related Report
Peer Reviewed
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