Functional significance and population structure of dominant fungal species in ectomycorrhizal communities in Japanese coastal pine forests

• Project/Area Number: 22688011
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Mie University
• Principal Investigator: MATSUDA Yosuke 三重大学, 大学院・生物資源学研究科, 准教授 (30324552)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 森林生態 / 保護 / 保全 / 海岸 / クロマツ / 菌根共生 / 外生菌根 / Cenococcum geophilum / 外生菌根菌 / 海岸林 / 耐塩性 / 耐熱性 / 遺伝子多様性 / マイクロサテライト / Cenococcum / internal transcribed spacer

Research Abstract

Coastal pine forests in Japan are declining by pine nematode disease and eradicated by a recent Tohoku earthquake needing effective planting techniques to rehabilitate the forests. Japanese black pine associate with ectomycorrhizal fungi that can facilitate nutrient water uptake and alleviate soil salinity.
I conducted the present research to clarify ectomycorrhizal community structures
genetic characterization of a dominant fungal species
and the response of the species against salinity.
Among 8 stands examined, Cenococcum geophilum was the most dominant species irrespective of the stands.Variations in the genetic diversities and the resistance to NaCl were observed among isolates of the species.

Research Products

• [Journal Article] Ito S (2013) Distribution of ectomycorrhizal fungi in a Chamaecyparis obtusa stand at different distances from a mature Quercus serrata tree (2013)
  • Author(s): Matsuda Y, Takano Y, Shimada M, Yamanaka T
  • Journal Title: Mycoscience Volume: 54 Pages: 260-264
  • Peer Reviewed
• [Journal Article] Tedersoo L (2013) Species richness and community composition of ectomycorrhizal fungi associated with alders (Alnus spp.) in relation to biotic and abiotic variables at the global scale (2013)
  • Author(s): Polme S, Bahram M, Yamanaka T, Nara K, Dai Y, Grebenc T, Kraigher H, Toivonen M, Wang P, Matsuda Y, Naadel T, Kennedy P, Koljalg U
  • Journal Title: New Phytologist Volume: 198 Pages: 1239-1249
  • Peer Reviewed
• [Journal Article] Ito S (2013) Enkianthus campanulatus (Ericaceae) is commonly associated with arbuscular mycorrhizal fungi (2013)
  • Author(s): Obase K, Matsuda Y
  • Journal Title: Mycorrhiza Volume: 23 Pages: 199-208
  • Peer Reviewed
• [Journal Article] Enkianthus campanulatus (Ericaceae) is commonly associated with arbuscular mycorrhizal fungi. (2013)
  • Author(s): Obase K, Matsuda Y, Ito S
  • Journal Title: Mycorrhiza Volume: 23 Issue: 3 Pages: 199-208
  • DOI: 10.1007/s00572-012-0462-y
  • Peer Reviewed
• [Journal Article] Distribution of ectomycorrhizal fungi in a Chamaecyparis obtusa stand at different distances from a mature Quercus serrata tree., (2013)
  • Author(s): Matsuda, Y, Takano, Y, Shimada M, Yamanaka T, Ito S
  • Journal Title: Mycoscience Volume: 未定 Issue: 4 Pages: 260-264
  • DOI: 10.1016/j.myc.2012.09.019
  • Peer Reviewed
• [Journal Article] Species richness and community composition of ectomycorrhizal fungi associated with alders (Alnus spp.) in relation to biotic and abiotic variables at the global scale. (2013)
  • Author(s): Polme S, Bahram M, Yamanaka T, Nara K, Dai Y, Grebenc T, Kraigher H, Toivonen M, Wang P, Matsuda Y, Naadel T, Kennedy P, Koljalg U, Tedersoo L
  • Journal Title: New Phytologist Volume: 未定 Issue: 4 Pages: 1239-1249
  • DOI: 10.1111/nph.12170
  • Peer Reviewed
• [Journal Article] Selosse M-A (2012) Seasonal and enverinmental changes of mycorrhizal associations and heterotrophy levels in mixotrophic Pyrola japonica growing under different light environments (2012)
  • Author(s): Matsuda Y, Shimizu S, Mori M, Ito S
  • Journal Title: accepted for American Journal of Botany Volume: 99 Pages: 1177-1188
  • Peer Reviewed
• [Journal Article] 菌類による放射性セシウムの吸収・蓄積.セミナー室「放射性降下物の農畜産水産物等への影響-4」 (2012)
  • Author(s): 齋藤雅典・山田明義・松田陽介・大和政秀
  • Journal Title: 化学と生物 Volume: 50 Pages: 748-751
• [Journal Article] Seasonal and enverinmental changes of mycorrhizal associations and heterotrophy levels in mixotrophic Pyrola japonica growing under different light environments (2012)
  • Author(s): Matsuda, Y., Shimizu, S., Mori, M., Ito, S., Selosse, M-A.
  • Journal Title: American Journal of Botany Volume: 99 Issue: 7 Pages: 1177-1188
  • DOI: 10.3732/ajb.1100546
  • Peer Reviewed
• [Journal Article] 菌類による放射性セシウムの吸収・蓄積．セミナー室「放射性降下物の農畜産水産物等への影響-4」 (2012)
  • Author(s): 齋藤雅典・山田明義・松田陽介・大和政秀
  • Journal Title: 化学と生物 Volume: 50 Pages: 748-751
• [Journal Article] Ito S (2011) Mycorrhizal fungi associated with Monotropastrum humile (Ericaceae) in central Japan (2011)
  • Author(s): Matsuda Y, Okochi S, Katayama T, Yamada, A
  • Journal Title: Mycorrhiza Volume: 21 Pages: 569-576
  • Peer Reviewed
• [Journal Article] 青森県と鹿児島県の海岸クロマツ林におけるCenococcum geophilumの出現状況 (2011)
  • Author(s): 松田陽介・木村ゆきな・中村克典・伊藤進一郎
  • Journal Title: 中部森林研究 Volume: 59 Pages: 239-242
  • Peer Reviewed
• [Journal Article] Mycorrhizal fungi associated with Monotropastrum humile (Ericaceae) in central Japan (2011)
  • Author(s): Matsuda Y, et al
  • Journal Title: Mycorrhiza Volume: 21 Issue: 6 Pages: 569-576
  • DOI: 10.1007/s00572-011-0365-3
  • Peer Reviewed
• [Journal Article] 国際集会開催の難しさ-MSJ/KSM Joint Symposium 2010を終えて- (2011)
  • Author(s): 松田陽介・大和政秀
  • Journal Title: 菌学会ニュースレター Volume: 2011-1 Pages: 4-6
• [Journal Article] ヒノキ林における外生菌根菌の分布
  • Author(s): 松田陽介
  • Journal Title: 広葉樹林化ハンドブック2012 Pages: 18-19
• [Journal Article] 外生菌根菌の放射能汚染に関わる諸問題
  • Author(s): 山田明義・松田陽介
  • Journal Title: 日本きのこ学会誌 Volume: 20 Pages: 154-157
  • NAID: 110009553918
• [Journal Article] 東海地方の海岸クロマツ林におけるCenococcum geophilumの出現割合
  • Author(s): 竹内裕也・松田陽介・伊藤進一郎
  • Journal Title: 中部森林研究 Volume: 58 Pages: 19-20
  • Peer Reviewed
• [Presentation] 海岸のクロマツ菌根に由来する Cenococcum geophilum の異なる温度条件下における菌糸伸長 (2013)
  • Author(s): 稲葉智美・松田陽介・小長谷啓介・伊藤進一郎
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2013-03-28
• [Presentation] 異なる塩化ナトリウム濃度が外生菌根菌Cenococcum geophilumの菌糸成長に及ぼす影響 (2013)
  • Author(s): 松田陽介・山川 舞・稲葉智美・小長谷啓介・伊藤進一郎
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2013-03-28
• [Presentation] Ito S (2013) Genetic diversity and population structure of Cenococcum geophilum at coastal pine forests in Japan (2013)
  • Author(s): Matsuda Y, Takeuchi K, Obase K
  • Organizer: Seventh International Conference on Mycorrhiza (ICOM7)
  • Place of Presentation: New Delih,インド
  • Year and Date: 2013-01-11
• [Presentation] Ito S (2012) Lessons from fungal inoculation experiments. How oak trees wilt and dead caused by the pathogen of the Japanese oak wilt (2012)
  • Author(s): Matsuda Y, Torii M, Yamada T
  • Organizer: International Symposium on Oak Forest Preservation
  • Place of Presentation: 韓国
  • Year and Date: 2012-08-29
• [Presentation] 日本の海岸クロマツ林におけるCenococcum geophilumの遺伝的多様 性 (2012)
  • Author(s): 松田陽介・竹内康介・小長谷啓介・伊藤進一郎
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(宇都宮市)
  • Year and Date: 2012-03-28
• [Presentation] 西ノ浜海岸のクロマツ菌根に由来するCenococcum geophilumの異なる温度条件下における菌糸伸長 (2012)
  • Author(s): 稲葉智美・松田陽介・小長谷啓介・伊藤進一郎
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(宇都宮 市)
  • Year and Date: 2012-03-28
• [Presentation] 日本の海岸クロマツ林におけるCenococcum geophilumの遺伝的多様性 (2012)
  • Author(s): 松田陽介, ら
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(宇都宮市)
  • Year and Date: 2012-03-28
• [Presentation] 西ノ浜海岸のクロマツ菌根に由来するCenococcum geophilumの異なる温度条件下における菌糸伸長 (2012)
  • Author(s): 稲葉智美, ら
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(宇都宮市)
  • Year and Date: 2012-03-28
• [Presentation] ツツジ科植物サラサドウダンにおけるアーバスキュラー菌根菌の多様性 (2012)
  • Author(s): 小長谷啓介, ら
  • Organizer: 第123回日本森林学会大会
  • Place of Presentation: 宇都宮大学(宇都宮市)
  • Year and Date: 2012-03-28
• [Presentation] Ito S (2012) How mycorrhizal networks contribute to keep biodiversity in forests (2012)
  • Author(s): Matsuda Y
  • Organizer: 2012 status and prospect of researches on forest biodiversity. Feb 3-4, 2012
  • Place of Presentation: Kangwon National University韓国
  • Year and Date: 2012-02-04
• [Presentation] How mycorrhizal networks contribute to keep biodiversity in forests? (2012)
  • Author(s): Matsuda Y, Ito S
  • Organizer: 2012 status and prospect of researches on forest biodiversity
  • Place of Presentation: Kangwon National University(韓国)(招待講演)
  • Year and Date: 2012-02-03
• [Presentation] 熱ストレスが外生菌根菌Cenococcum geophilumの成長に及ぼす影響 (2011)
  • Author(s): 稲葉智美, ら
  • Organizer: 第1回中部森林学会
  • Place of Presentation: 石川県地場産業振興センター(金沢市)
  • Year and Date: 2011-10-22
• [Presentation] 愛知県西ノ浜海岸のクロマツ林から採取した外生菌根菌Cenococcum geophilumの遺伝的構造 (2011)
  • Author(s): 竹内康介, ら
  • Organizer: 第1回中部森林学会
  • Place of Presentation: 石川県地場産業振興センター(金沢市)
  • Year and Date: 2011-10-22
• [Presentation] ヒノキ人工林に生息する外生菌根菌群集 (2011)
  • Author(s): 松田陽介, ら
  • Organizer: 日本菌学会第55回大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-10
• [Presentation] ツツジ科ドウダンツツジ属植物の菌根形成 (2011)
  • Author(s): 小長谷啓介, ら
  • Organizer: 日本菌学会第55回大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-10
• [Presentation] Ito S (2011) Dominance of an ectomycorrhizal fungus Cenococcum geophilum in coastal Japanese black pine forests in Japan (2011)
  • Author(s): Matsuda Y, Obase K, Kimura Y, Takeuchi Y
  • Organizer: Asian mycological congress 2011
  • Place of Presentation: Incheon University,韓国
  • Year and Date: 2011-08-11
• [Presentation] 青森県と鹿児島県における海岸クロマツ林の外生菌根群集 (2011)
  • Author(s): 松田陽介・木村ゆきな・伊藤進一郎
  • Organizer: 第122回日本森林学会大会
  • Place of Presentation: 静岡大学(静岡市)
  • Year and Date: 2011-03-26
• [Presentation] Dominance of an ectomycorrhizal fungus Cenococcum geophilum in coastal Japanese black pine forests in Japan (2011)
  • Author(s): Matsuda Y, et al
  • Organizer: Asian mycological congress 2011
  • Place of Presentation: インチョン大学(韓国)
• [Presentation] 青森県と鹿児島県の海岸クロマツ林におけるCenococcum geophilumの出現状況 (2010)
  • Author(s): 木村ゆきな, ら
  • Organizer: 第59回日本林学会中部支部大会
  • Place of Presentation: 三重大学(津市)
  • Year and Date: 2010-10-16
• [Presentation] Kubota N Ito S (2010) Changes in ectomycorrhizal communities with stand ages at Japanese coastal pine forests (2010)
  • Author(s): Matsuda Y
  • Organizer: Organic matter stabilization and ecosystem functions
  • Place of Presentation: Hyeres,フランス
  • Year and Date: 2010-09-23
• [Presentation] ラン科植物キンランに定着する菌根菌種の特異性 (2010)
  • Author(s): 松田陽介, ら
  • Organizer: 日本菌学会第54回大会
  • Place of Presentation: 玉川大学(東京都)
  • Year and Date: 2010-05-30
• [Presentation] 中部地方における海岸クロマツ林の外生菌根群集 (2010)
  • Author(s): 松田陽介, ら
  • Organizer: 第121回日本森林学会大会
  • Place of Presentation: 筑波大学(つくば市)
  • Year and Date: 2010-04-03
• [Presentation] Changes in ectomycorrhizal communities with stand ages at Japanese coastal pine forests (2010)
  • Author(s): Matsuda, Y., et al
  • Organizer: Organic matter stabilization and ecosystem functions
  • Place of Presentation: Hyeres, France
• [Presentation] ツツジ科植物サラサドウダンの根から分離培養された根内内生菌相
  • Author(s): 小長谷啓介・松田陽介・伊藤進一郎
  • Organizer: 日本菌学会第56回大会
  • Place of Presentation: 岐阜大学 (岐阜市)
• [Presentation] 海岸クロマツ林におけるCenococcum geophilumの遺伝的構造
  • Author(s): 松田陽介・竹内康介・小長谷啓介・伊藤進一郎
  • Organizer: 日本菌学会第56回大会
  • Place of Presentation: 岐阜大学 (岐阜市)
• [Presentation] 異なる光条件下に生育するイチヤクソウの菌根共生と従属栄養性の季節変化
  • Author(s): 松田陽介・清水瞳子・森万菜実・伊藤進一郎・Selosse MA
  • Organizer: 第60回日本生態学会
  • Place of Presentation: グランシップ静岡（静岡市）
• [Presentation] ツツジ科植物の進化過程における菌根菌の変遷に関する一考察
  • Author(s): 小長谷啓介・松田陽介
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] 異なる塩化ナトリウム濃度が外生菌根菌Cenococcum geophilumの菌糸成長に及ぼす影響
  • Author(s): 松田陽介・山川 舞・稲葉智美・小長谷啓介・伊藤進一郎
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] 海岸のクロマツ菌根に由来する Cenococcum geophilum の 異なる温度条件下における菌糸伸長
  • Author(s): 稲葉智美・松田陽介・小長谷啓介・伊藤進一郎
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] Convergent evolution of heterotrophy in mycorrhizal symbioses of Monotropoideae (Ericaceae) and Orchidaceae
  • Author(s): Selosse M-A, Matsuda Y, Palancade C, HashimotoY
  • Organizer: 7th International Symbiosis Society Congress
  • Place of Presentation: Kraków, Poland
• [Presentation] Lessons from fungal inoculation experiments. How oak trees wilt and dead caused by the pathogen of the Japanese oak wilt?
  • Author(s): Matsuda, Y., Torii, M., Yamada, T., Ito, S
  • Organizer: International Symposium on Oak Forest Preservation
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Genetic diversity and population structure of Cenococcum geophilum at coastal pine forests in Japan
  • Author(s): Matsuda Y, Takeuchi K, Obase K, Ito S
  • Organizer: Seventh International Conference on Mycorrhiza
  • Place of Presentation: New Delih, India
• [Presentation] Mycorrhizal status of Enkianthus campanulatus (Ericaceae)
  • Author(s): Obase K, Matsuda Y
  • Organizer: Seventh International Conference on Mycorrhiza
  • Place of Presentation: New Delih, India
• [Book] 広葉樹林化ハンドブック2012，IV-3. ヒノキ林における外生菌根菌の分布 (2012)
  • Author(s): 松田陽介
  • Total Pages: 48
  • Publisher: 独立行政法人 森林総合研究所四国支所編
• [Remarks]
  • URL: http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab2/matsuda.htm
• [Remarks] 松田陽介
  • URL: http://www.bio.mie-u.ac.jp/junkan/busshitsu/lab2/matsuda.htm