The role of membrane nanotubes in the immune system

• Project/Area Number: 22689017
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The University of Tokyo (2012); The Institute of Physical and Chemical Research (2010-2011)
• Principal Investigator: HASE Koji 東京大学, 医科学研究所, 特任教授 (20359714)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000); Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 粘膜免疫 / 細胞間情報伝達 / 膜ナノチューブ / M-Sec / RalA / TNFaip2 / Exocyst複合体 / Exocyst complex / M細胞

Research Abstract

Cell-to-cell communication is essential for the development and maintenance of multicellular organisms. The tunneling nanotube (TNT) is a recently recognized distinct type of intercellular communication device. We have recently shown that M-Sec (also called TNFaip2) is a key molecule for TNT formation. We also elucidated the molecular machinery of M-Sec-dependent TNT formation.

Research Products

• [Journal Article] Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice (2013)
  • Author(s): Okada T
  • Journal Title: Nat. Commun Volume: 4巻 Issue: 1 Pages: 1654-1654
  • DOI: 10.1038/ncomms2668
  • Peer Reviewed
• [Journal Article] Epithelial cell-intrinsic notch signaling plays an essential role in the maintenance of gut immune homeostasis (2012)
  • Author(s): Obata Y
  • Journal Title: J. Immunol Volume: 188巻 Pages: 2427-2436
  • Peer Reviewed
• [Journal Article] Critical role of the IgM Fc receptor in IgM homeostasis, B cell survival and humoral immune responses (2012)
  • Author(s): Ouchida, R., Mori, H., Hase, K., Takatsu, H., Kurosaki, T., Tokuhisa, T., Ohno, H., Wang. J. -Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Issue: 40 Pages: 2699-2706
  • DOI: 10.1073/pnas.1210706109
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K. A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O., Toyooka, K., Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S. J., Kaisho, T., Williams, I. R., and Ohno, H.
  • Journal Title: Nat Immunol Volume: 13 Issue: 8 Pages: 729-736
  • DOI: 10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] Brucella abortus exploits a cellular prion protein on intestinal M cells as an invasive receptor. (2012)
  • Author(s): Nakato G, Hase K, Suzuki M, Kimura M, Ato M, Hanazato M, Tobiume M, Horiuchi M, Atarashi R, Nishida N, Watarai M, Imaoka K, Ohno H
  • Journal Title: Journal of Immunology Volume: 189(4) Issue: 4 Pages: 1540-4
  • DOI: 10.4049/jimmunol.1103332
  • Peer Reviewed
• [Journal Article] Epithelial cell-intrinsic notch signaling plays an essential role in the maintenance of gut immune homeostasis (2012)
  • Author(s): Obata Y., et al
  • Journal Title: J.Immunol. Volume: 188 Issue: 5 Pages: 2427-2436
  • DOI: 10.4049/jimmunol.1101128
  • Peer Reviewed
• [Journal Article] The Epithelia-specific membrane trafficking factor AP-1B controls gut immune homeostasis in mice (2011)
  • Author(s): Takahashi D. and Hase K
  • Journal Title: Gastroenterology Volume: 141巻 Pages: 621-632
  • Peer Reviewed
• [Journal Article] Application of a mouse ligated Peyer's patch intestinal loop assay to evaluate bacterial uptake by M cells (2011)
  • Author(s): Fukuda S., Hase K., et al
  • Journal Title: J.Vis.Exp. Volume: Dec17 Issue: 58
  • DOI: 10.3791/3225
  • Peer Reviewed
• [Journal Article] Bifidobacteria protect host from enteropathogenic infection through production of acetate (2011)
  • Author(s): Fukuda S
  • Journal Title: Nature Volume: 469巻 Issue: 7331 Pages: 543-547
  • DOI: 10.1038/nature09646
  • Peer Reviewed
• [Journal Article] CCR6hiCD11cint B cells promote M-cell differentiation in Peyer's patch (2011)
  • Author(s): Ebisawa, M., et al
  • Journal Title: Int.Immunol. Volume: 23 Issue: 4 Pages: 261-269
  • DOI: 10.1093/intimm/dxq478
  • Peer Reviewed
• [Journal Article] The epithelia-specific membrane trafficking factor AP-1B controls gut immune homeostasis in mice (2011)
  • Author(s): Takahashi, D., et al
  • Journal Title: Gastroenterology Volume: 14 Issue: 2 Pages: 621-632
  • DOI: 10.1053/j.gastro.2011.04.056
  • Peer Reviewed
• [Journal Article] AP1B plays an important role in intestinal tumorigenesis with the truncating mutation of an APC gene (2011)
  • Author(s): Mimura M., et al
  • Journal Title: J.Vis.Exp. Volume: Dec 17
  • Peer Reviewed
• [Journal Article] Bifidobacteria protect host from enteropathogenic infection through production of acetate (2011)
  • Author(s): Fukuda S., et al.
  • Journal Title: Nature Volume: 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] CCR6^<hi>CD11c^<int> B cells promote M-cell differentiation in Peyer's patch (2011)
  • Author(s): Ebisawa M., Hase K., et al.
  • Journal Title: Int.Immunol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] M-Sec a key molecule for tunneling nanotubule formation (2010)
  • Author(s): Ohno H. and Hase K
  • Journal Title: Communicat. Integrat. Biol Volume: 3巻 Pages: 231-233
• [Journal Article] M-Sec, a key molecule for tunneling nanotubule formation. (2010)
  • Author(s): Ohno H., Hase K., et al.
  • Journal Title: Communicat.Integrat.Biol. Volume: 3 Pages: 231-233
• [Presentation] Epithelial Notch signaling secures lymphoid organogenesis and immune homeostasis in the gut. (2012)
  • Author(s): Hase K.
  • Organizer: 2012 Annual Meeting of the Japanese Society for Immunology.
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Roles of intestinal M cells in mucosal infection and immunity. (2012)
  • Author(s): Hase K.
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] 粘膜表面の感染と宿主応答に果たすＭ細胞の役割 (2012)
  • Author(s): 長谷耕二
  • Organizer: 第一回川島腎カンファレンス
  • Place of Presentation: 各務原
  • Invited
• [Presentation] Intestinal microbiota shapes intestinal T cell balance via epigenetic modifications (2012)
  • Author(s): Hase K.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Invited
• [Presentation] 感染マトリクス成果報告研究項目(B)細菌 (2011)
  • Author(s): 長谷耕二
  • Organizer: 感染現象のマトリックスシンポジウム「感染症研究の未来」
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] 腸管粘膜表面の免疫監視におけるM細胞の重要性の解明 (2011)
  • Author(s): 長谷耕二
  • Organizer: 第15回腸内細菌学会
  • Place of Presentation: 東京(招待講演)
• [Presentation] Epithelial immune functions at the interface between self and non-self (2011)
  • Author(s): Koji Hase
  • Organizer: 2nd CSI/JSI/KAI Joint Symposium on Immunology
  • Place of Presentation: 東京(招待講演)
• [Presentation] Specialized epithelial M cells contribute to immunosurveillance on the mucosal surface (2010)
  • Author(s): Hase K
  • Organizer: The Japanese Society for Immunology 2010 Symposium for Cutting edge of immunology; innate immunity and immuneregulation
  • Place of Presentation: 東京
  • Year and Date: 2010-12-03
• [Presentation] Specialized epithelial M cells contribute to immunosurveillance on the mucosal surface. (2010)
  • Author(s): Hase K.
  • Organizer: The Japanese Society for Immunology 2010 Symposium for "Cutting edge of immunology ; innate immunity and immune regulation"
  • Place of Presentation: 東京
  • Year and Date: 2010-12-03
• [Presentation] Essential Role of Membrane Trafficking Factor AP-1B in Gut Immune Homeostasis (2010)
  • Author(s): Hase K., et al.
  • Organizer: Biochemistry and Molecular Biology 2010
  • Place of Presentation: 神戸
• [Presentation] M-Sec sec-ures membrane nanotubes with RalA and exocyst (2010)
  • Author(s): Hase K.
  • Organizer: International Symposium on Organelle Network : Microbiology, Immunology & Cell Biology
  • Place of Presentation: 大阪
• [Presentation] 特殊上皮M細胞による粘膜表面の免疫監視機構 (2010)
  • Author(s): 長谷耕二
  • Organizer: 第16回 日本ヘリコバクター学会学術集会
  • Place of Presentation: 京都
• [Presentation] Antigen transcytosis by M cells : from molecular entities to biological significance (2010)
  • Author(s): Hase K.
  • Organizer: 4^<th> Symposium of Immunology Frontier Research Center (IFReC)
  • Place of Presentation: 大阪
• [Presentation] Epithelial immune functions at the interface between self and non-self.
  • Author(s): Hase K.
  • Organizer: IEIIS2012 Homeostatic Inflammation Satellite Symposium - Infection, Inflammation and Immunity.
  • Place of Presentation: 東京
  • Invited
• [Book] 臨床粘膜免疫学 (2010)
  • Author(s): 清野宏編集
  • Total Pages: 722
  • Publisher: シナジー出版
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/dmb/index.html