Virotherapy for bone and soft tissue sarcoma by telomerase-specific oncolytic adenovirus

• Project/Area Number: 22689040
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Niigata University
• Principal Investigator: KAWASHIMA Hiroyuki 新潟大学, 医歯学系, 助教 (30361900)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 骨 / 軟部腫瘍学 / 軟部肉腫 / ウイルス療法 / アデノウイルス / テロメラーゼ / 骨肉腫

Research Abstract

Bone and soft tissue sarcoma cells treated by telomerase-specific oncolytic adenovirus showed a dose- and time-dependent viral replication and cytolysis, which were positively associated with the expression levels of coxackie-adenoviral receptor and hTERT. Oncolytic adenoviral infection also induced both apototic and autophagic cell death in these cells, and also showed inhibition of tumor growth in osteosarcoma xenograft mouse model.

Research Products

• [Journal Article] Telomelysin exhibits potent anti-tumor activity via apoptotic and non-apoptotic cell death in softtissue sarcoma cells (2013)
  • Author(s): Guidong Li, Hiroyuki Kawashima, et al
  • Journal Title: Cancer Sci Volume: (in press)
  • Peer Reviewed
• [Journal Article] Efficient virotherapy for osteosarcoma by telomerase-specific oncolytic adenovirus (2011)
  • Author(s): Guidong Li, Hiroyuki Kawashima, et al
  • Journal Title: J Cancer ResClin Oncol Volume: 137 Issue: 6 Pages: 1037-1051
  • DOI: 10.1007/s00432-010-0969-6
  • Peer Reviewed
• [Journal Article] Efficient virotherapy for osteosarcoma by telomerase-specific oncolytic adenovirus.
  • Author(s): Guidong Li, et al.
  • Journal Title: Journal of Cancer Research and Clinical Oncology Volume: (In press)
  • Peer Reviewed
• [Presentation] Telomerase-specific oncolytic adenovirus exhibits potent anti-tumor activity, via induction of both apoptotic and non-apoptotic cell death in soft tissue sarcoma cells (2011)
  • Author(s): Guidong Li, Hiroyuki Kawashima, et al
  • Organizer: 16^<th> ISOLS general meeting
  • Place of Presentation: Beijing, China
  • Year and Date: 2011-09-17
• [Presentation] ヒト軟部肉腫におけるテロメラーゼ特異的制限増殖型アデノウイルス、テロメライシン(OBP-301)の抗腫瘍効果 (2010)
  • Author(s): 李貴東、川島寛之ほか
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-14
• [Presentation] ヒト軟部肉腫におけるテロメラーゼ特異的制限増殖型ウイルス、テロメライシン(OBP-301)の抗腫瘍効果 (2010)
  • Author(s): 李貴東, ほか
  • Organizer: 第25回日本整形外科学会基礎学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-10-14
• [Presentation] ヒト骨肉腫細胞株およびマウス移植モデルにおけるテロメラーゼ特異的制限増殖型ウイルスの抗腫瘍効果 (2010)
  • Author(s): 李貴東、川島寛之ほか
  • Organizer: 第43回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-15
• [Presentation] ヒト骨肉腫細胞株およびマウス移植モデルにおけるテロメラーゼ特異的制限増殖型ウイルスの抗腫瘍効果 (2010)
  • Author(s): 李貴東, ほか
  • Organizer: 第43回日本整形外科学会 骨・軟部腫瘍学術集会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-07-15
• [Presentation] Telomerase-specific oncolytic virotherapy for osteosarcoma (2010)
  • Author(s): Hiroyuki Kawashima, et al
  • Organizer: 101^<st> AACR annual meeting
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2010-04-19