The role of epogenetic modulation in the neuropathic pain
| Project/Area Number |
22689043
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
NAKAE Aya 大阪大学, 医学(系)研究科(研究院), 准教授 (60379170)
|
|---|
| Project Period (FY) |
2010-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
|
|---|
| Keywords | 遺伝子後修飾 / 神経障害性疼痛 / バイオマーカー / DNA後修飾 / BDNF / 神経障害痛 / 動物モデル / 眼窩下神経絞扼モデル / メチル化 / 大規模シークエンサー / スプライスバリアント / 定量PCR / エピジェネティック / ヒストン修飾 / 神経因性疼痛 |
|---|
| Research Abstract |
Pain is one of the most uncomfortable feeling to have of humans, but the mechanism is unclear.In this study, we conducted research by focusing on the epigenetic modification. We focused on the unique feature of brain-derived neurotrophic factor(BDNF)gene construction, we performed the mRNA quantification of the various splice variants, methylation analysis of CpG islands of each splice variant, and histone modification. Many CpG islands methylation rates changes were very little but significantly higher in nerve-injured rats than naive, or naive and sham rats. The dendrogram from methylation profiles of some exon's promoter region demonstrated that classification of nerve injured rats and naive controls at the first branch completely matched the condition. These were considered to be strong candidates for biomarkers of neuropathic pain in the future.
|
Report
(5 results)
Research Products
(57 results)
-
-
-
-
-
-
-
[Journal Article] Recovery of motoneuron and locomotor function after chronic spinal cord injury depends on constitutive activity in 5-HT2C receptors2010
Author(s)
Murray K, Nakae A, Stephens MJ, Rank M, D'Amico J, Harvey P, Li X, Harris L, Ballou EW, Anelli R, Heckman CJ, Mashimo T, Vavrek R, Sanelli L, Gorassini MA, Bennett DJ and Fouad K
-
Journal Title
Nature Medicine
Volume: 16(8)
Pages: 694-700
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Pain Modulation by Negative and Positive Emotional Sounds
Author(s)
Nakae A, Hermann T, Ikeda T, Sakurai T, Adachi T, Ito H, Nishimura T, Shibata M, Osaka M, Mashimo T
Organizer
4th INTERNATIONAL CONGRESS ON NEUROPATHIC PAIN(IASP)
Place of Presentation
Toronto, Canada
Related Report
-
-
-
-
