| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
|
|---|
| Research Abstract |
Pain is one of the most uncomfortable feeling to have of humans, but the mechanism is unclear.In this study, we conducted research by focusing on the epigenetic modification. We focused on the unique feature of brain-derived neurotrophic factor(BDNF)gene construction, we performed the mRNA quantification of the various splice variants, methylation analysis of CpG islands of each splice variant, and histone modification. Many CpG islands methylation rates changes were very little but significantly higher in nerve-injured rats than naive, or naive and sham rats. The dendrogram from methylation profiles of some exon's promoter region demonstrated that classification of nerve injured rats and naive controls at the first branch completely matched the condition. These were considered to be strong candidates for biomarkers of neuropathic pain in the future.
|
