• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles of gap junction proteins in odontoblast differentiation

Research Project

Project/Area Number 22689053
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Orthodontic/Pediatric dentistry
Research InstitutionThe University of Tokushima (2013)
Tohoku University (2010-2012)

Principal Investigator

IWAMOTO Tsutomu  徳島大学, ヘルスバイオサイエンス研究部, 教授 (90346916)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Keywords歯胚発生 / 象牙芽細胞 / 細胞間結合 / パネキシン3 / 歯の発生 / 前象牙芽細胞 / パネキシン
Research Abstract

In this project, we had performed bioinformatical analysis to find the responsible gene which regulate odontoblast differentiation. We had found that pannexin3, a new member of gap junction protein, pannexin family, was preferentially expressed in tooth cDNA library. In fact, in situ hybridization and immunostaining revealed that pannexin3 was expressed in preoodontoblast layer. To analyze the function of pannexin3 in odontoblast, mDP cell, a mouse dental papilla derived cell line, was used in this study. We found that pannexin3 regulate cell proliferation and differentiation by releasing intracellular ATP and activating AMPK signaling.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (41 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (29 results)

  • [Journal Article] Pannexin 3 inhibits proliferation of osteoprogenitor cells by regulating Wnt and p21 signaling2014

    • Author(s)
      Ishikawa M, Iwamoto T, Fukumoto S, Yamada Y
    • Journal Title

      J Biol Chem

      Volume: 289(5) Issue: 5 Pages: 2839-2851

    • DOI

      10.1074/jbc.m113.523241

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 象牙芽細胞の分化と細胞外基質2014

    • Author(s)
      岩本 勉
    • Journal Title

      四国歯学会雑誌

      Volume: 26(2) Pages: 23-28

    • NAID

      120005832492

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Application of a tooth-surface coating material to teeth with discolored crowns2013

    • Author(s)
      Hashimura T, Yamada A, Iwamoto T, Arakaki M, Saito K, Fukumoto S.
    • Journal Title

      PEDIATRIC DENTAL JOURNAL

      Volume: 23(1) Pages: 44-50

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological roles of gap junction proteins in cartilage and bone development2013

    • Author(s)
      Iwamoto T, Ishikawa M, Ono M, Nakamura T,Fukumoto S, Yamada Y
    • Journal Title

      J Oral Biosci

      Volume: 26(2) Pages: 23-28

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation2012

    • Author(s)
      Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
    • Journal Title

      J Biol Chem

      Volume: 287(13) Issue: 13 Pages: 10590-601

    • DOI

      10.1074/jbc.m111.285874

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycosphingolipids regulate ameloblastin expression in dental epithelial cells2012

    • Author(s)
      Kamasaki, et al
    • Journal Title

      Journal of Dental Research

      Volume: 91 Issue: 1 Pages: 78-83

    • DOI

      10.1177/0022034511424408

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epithelial-mesenchymal interaction reduces inhibitory effects of fluoride on proliferation and enamel matrix expression in dental epithelial cells2012

    • Author(s)
      Yamada A, Iwamoto T, et al
    • Journal Title

      PEDIATRIC DENTAL JOURNAL

      Volume: 22(1) Pages: 1-9

    • NAID

      10030558189

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation2011

    • Author(s)
      Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S, Yamada Y
    • Journal Title

      J Cell Biol

      Volume: 27; 193(7) Pages: 1257-1274

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation2011

    • Author(s)
      Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S, Yamada Y
    • Journal Title

      J Cell Biol

      Volume: 27;193(7) Issue: 7 Pages: 1257-74

    • DOI

      10.1083/jcb.201101050

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expressions and Functions of Neurotrophic Factors in Tooth Development2011

    • Author(s)
      Iwamoto T, et.al.
    • Journal Title

      J Oral Biosci

      Volume: 53(1) Pages: 113-121

    • NAID

      10027793552

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation2010

    • Author(s)
      Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y
    • Journal Title

      J Biol Chem

      Volume: 285(24) Issue: 24 Pages: 18948-58

    • DOI

      10.1074/jbc.m110.127027

    • Related Report
      2013 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PDGFs regulate tooth germ proliferation and ameloblast differentiation2010

    • Author(s)
      Nun W, Iwamoto T, Sugawara Y, Futaki M, Yoshizaki K, Yamamoto S, Yamada A, Nakamura T, Nonaka K, and Fukumoto S
    • Journal Title

      Archives of Oral Biology

      Volume: 55(6) Pages: 426-434

    • Related Report
      2013 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 歯から全身へー歯髄幹細胞の可能性-2013

    • Author(s)
      岩本 勉
    • Organizer
      第32回日本小児歯科学会中四国地方会
    • Place of Presentation
      岡山大学創立五十周年記念館(岡山県)
    • Year and Date
      2013-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト乳歯歯髄細胞におけるSDF-1の発現調節機構について2013

    • Author(s)
      長谷川智一, 赤澤友基, 帖佐直幸, 吉村善隆, 浅川剛吉, 石崎 明, 岩本 勉
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山県)
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨・軟骨形成過程におけるギャップ結合分子パネキシン3の機能解析2013

    • Author(s)
      岩本 勉
    • Organizer
      第55回歯科基礎医学会学術大会・総会サテライトシンポジウム
    • Place of Presentation
      岡山コンベンションセンター(岡山県)
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 唾液腺発生過程におけるConnexin43-FGF10 シグナルの解析2013

    • Author(s)
      福本 敏, 二木正晴, 岩本 勉, 中村卓史, 山田亜矢
    • Organizer
      第51回日本小児歯科学会大会
    • Place of Presentation
      長良川国際会議場(長野県)
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 唾液腺発生過程におけるConnexin43-FGF10シグナルの解析2013

    • Author(s)
      福本 敏、二木正晴、岩本 勉、中村卓史、山田亜矢
    • Organizer
      第51回日本小児歯科学会大会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨・軟骨形成過程におけるギャップ結合分子パネキシン3の機能解析2013

    • Author(s)
      岩本 勉
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山コンベンションセンター(岡山県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Pannexin 3, a gap junction protein, regulates odontoblast differentiation2012

    • Author(s)
      Iwamoto T, Ishikawa M, Yoshizaki K, Yamada A, Nakamura T, Yamada Y, Fukumoto S
    • Organizer
      The 90th General Session & Exhibition of the International Association of Dental Research (IADR)
    • Place of Presentation
      Iguacu Falls Convention Center(イグアスフォールズ, ブラジル)
    • Year and Date
      2012-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] エナメル芽細胞分化過程におけるテトラスパニンCD9の発現とその役割2012

    • Author(s)
      岩本 勉, 小野真理子, 二木正晴, 菅原優, 山田亜矢, 中村卓史, 福本 敏
    • Organizer
      第50回日本小児歯科学会大会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Year and Date
      2012-05-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Pannexin 3, a gap junction protein, regulates proliferation through AMPK pathway in odontoblasts2012

    • Author(s)
      Iwamoto T, Ishikawa M, Yoshizaki K, Nakamura T, Yamada A, Yamada Y, Fukumoto S
    • Organizer
      2012 American society for Biochemistry and Molecular Biology Annual meeting
    • Place of Presentation
      San Diego Convention Center, USA(サンディエゴ, 米国)
    • Year and Date
      2012-04-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Pannexin 3, a gap junction protein, regulates proliferation through AMPK pathway in odontoblasts2012

    • Author(s)
      Tsutomu Iwamoto
    • Organizer
      2012 American society for Biochemistry and Molecular Biology Annual meeting
    • Place of Presentation
      San Diego Convention Center, 米国
    • Related Report
      2012 Annual Research Report
  • [Presentation] エナメル芽細胞分化過程におけるテトラスパニンCD9の発現とその役割2012

    • Author(s)
      岩本 勉
    • Organizer
      第50回日本小児歯科学会大会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pannexin 3, a gap junction protein, regulates odontoblast differentiation.2012

    • Author(s)
      Tsutomu Iwamoto
    • Organizer
      The 90th General Session & Exhibition of the International Association of Dental Research
    • Place of Presentation
      Iguacu Falls Convention Center, ブラジル
    • Related Report
      2012 Annual Research Report
  • [Presentation] 象牙芽細胞分化制御機構の同定とその応用2011

    • Author(s)
      岩本 勉
    • Organizer
      第49回日本小児歯科学会大会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-11-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 象牙芽細胞分化制御機構の同定とその応用2011

    • Author(s)
      岩本勉
    • Organizer
      第49回日本小児歯科学会大会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ
    • Year and Date
      2011-11-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 象牙芽細胞分化における細胞外マトリックス(ECM)の役割2011

    • Author(s)
      岩本 勉
    • Organizer
      第53回歯科基礎医学会学術大会・総会
    • Place of Presentation
      長良川国際会議場(長野県)
    • Year and Date
      2011-10-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 象牙芽細胞分化における細胞外マトリックス(ECM)の役割2011

    • Author(s)
      岩本勉
    • Organizer
      第53回歯科基礎医学会学術大会・総会
    • Place of Presentation
      長良川国際会議場
    • Year and Date
      2011-10-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pannexin 3 plays a critical role for odontoblast differentiation2011

    • Author(s)
      Iwamoto T, Sugawara Y, Ono M, Yamada A, Nakamura T, Fukumoto S
    • Organizer
      American Society For Bone And Mineral Research (ASBMR) 2011 Annual Meeting
    • Place of Presentation
      San Diego Convention Center, USA(サンディエゴ, 米国)
    • Year and Date
      2011-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Pannexin 3 plays a critical role for odontoblast differentiation2011

    • Author(s)
      Iwamoto T, et al
    • Organizer
      American Society For Bone And Mineral Research (ASBMR) 2011 Annual Meeting
    • Place of Presentation
      San Diego Convention Center, USA
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 歯および軟骨細胞におけるPannexin 3 の役割2011

    • Author(s)
      岩本 勉, 福本 敏
    • Organizer
      第9回口腔医科学フロンティア学術集会
    • Place of Presentation
      九州大学歯学部本館1階(福岡県)
    • Year and Date
      2011-03-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯および軟骨細胞におけるPannexin 3の役割2011

    • Author(s)
      岩本勉, 他
    • Organizer
      第9回口腔医科学フロンティア学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] GSK-3β抑制剤を用いた歯髄細胞の未分化誘導法の確立2011

    • Author(s)
      福本敏、山田亜矢、岩本勉, 他
    • Organizer
      第3回口腔先端応用医科学研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] New gap junctional protein pannexin 3 regulates tooth, cartilage and bone development2011

    • Author(s)
      Iwamoto T, Ono M, Arakaki M, Nakamura T, Yamada A, Fukumoto S
    • Organizer
      Harvard-Forsyth-Tohoku Research Workshop
    • Place of Presentation
      Belfer Case Study Room, CGIS South, Harvard University(ボストン, 米国)
    • Year and Date
      2011-01-07
    • Related Report
      2013 Final Research Report
  • [Presentation] New gap junctional protein pannexin 3 regulates tooth, cartilage and bone development2011

    • Author(s)
      Iwamoto T, et.al.
    • Organizer
      Harvard-Forsyth-Tohoku Research Workshop
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-01-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pannexin 3 coordinates odontoblasts proliferation and differentiation by cell-matrix interaction2010

    • Author(s)
      二木正晴, 岩本勉, 他
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析2010

    • Author(s)
      岩本 勉, 中村卓史, 吉崎恵悟, 山田亜矢, 福本 敏
    • Organizer
      第52回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      タワーホール船堀(千葉県)
    • Year and Date
      2010-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析2010

    • Author(s)
      岩本勉, 他
    • Organizer
      第52回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      船堀
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 神経栄養因子による歯原性上皮細胞の増殖、分化制御機構の解明2010

    • Author(s)
      小野真理子、岩本勉, 他
    • Organizer
      第52回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      船堀
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 乳歯歯髄細胞による細胞走化性因子の調節機構

    • Author(s)
      長谷川智一、赤澤友基、中川 弘、上田公子、岩本 勉
    • Organizer
      第31回日本小児歯科学会北日本地方会
    • Place of Presentation
      ねぶたの家 ワ・ラッセ(青森県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 歯から全身へー歯髄幹細胞の可能性―

    • Author(s)
      岩本 勉
    • Organizer
      第32回日本小児歯科学会中四国地方会
    • Place of Presentation
      岡山大学創立五十周年記念館(岡山県)
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi