Roles of gap junction proteins in odontoblast differentiation
Project/Area Number |
22689053
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Orthodontic/Pediatric dentistry
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Research Institution | The University of Tokushima (2013) Tohoku University (2010-2012) |
Principal Investigator |
IWAMOTO Tsutomu 徳島大学, ヘルスバイオサイエンス研究部, 教授 (90346916)
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Project Period (FY) |
2010-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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Keywords | 歯胚発生 / 象牙芽細胞 / 細胞間結合 / パネキシン3 / 歯の発生 / 前象牙芽細胞 / パネキシン |
Research Abstract |
In this project, we had performed bioinformatical analysis to find the responsible gene which regulate odontoblast differentiation. We had found that pannexin3, a new member of gap junction protein, pannexin family, was preferentially expressed in tooth cDNA library. In fact, in situ hybridization and immunostaining revealed that pannexin3 was expressed in preoodontoblast layer. To analyze the function of pannexin3 in odontoblast, mDP cell, a mouse dental papilla derived cell line, was used in this study. We found that pannexin3 regulate cell proliferation and differentiation by releasing intracellular ATP and activating AMPK signaling.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation2012
Author(s)
Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
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Journal Title
J Biol Chem
Volume: 287(13)
Issue: 13
Pages: 10590-601
DOI
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Peer Reviewed
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[Presentation] Pannexin 3, a gap junction protein, regulates proliferation through AMPK pathway in odontoblasts2012
Author(s)
Iwamoto T, Ishikawa M, Yoshizaki K, Nakamura T, Yamada A, Yamada Y, Fukumoto S
Organizer
2012 American society for Biochemistry and Molecular Biology Annual meeting
Place of Presentation
San Diego Convention Center, USA(サンディエゴ, 米国)
Year and Date
2012-04-25
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