Molecular codes for neuronal individuality in neural circuit formation

• Project/Area Number: 22700326
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Gunma University
• Principal Investigator: KANEKO Ryosuke 群馬大学, 大学院・医学系研究科, 助教 (40390695)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経発生 / 多様性 / 神経回路 / バスケット細胞 / プロトカドヘリン / GABAニューロン / 小脳 / 遺伝子改変マウス / 進化

Research Abstract

We have generated transgenic mice that genetically label individual cerebellar GABAergic neurons with red fluorescence. Moreover, several lines of clustered Pcdhs deficient mice have been generated and the Pcdh-a & b deficient mice showed motor abnormalities. These experiments support our hypothesis that protocadherins provide molecular codes for neuronal individuality in neural circuit formation.

Research Products

• [Journal Article] Electrophysiological characteristics of inhibitory neurons of the prepositus hypoglossi nucleus as analyzed in Venus-expressing transgenic ratsregions. (2011)
  • Author(s): Masato Shino, Ryosuke Kaneko(co-first author), Yuchio Yanagawa, Yasuo Kawaguchi, Yasuhiko Saito
  • Journal Title: Neuroscience Volume: 197 Pages: 89-98
  • Peer Reviewed
• [Journal Article] Identification of the Cluster Control Region for the Protocadherin-β Genes Located beyond the Protocadherin-γ Cluster (2011)
  • Author(s): Shinnichi Yokota, Teruyoshi Hirayama, Keizo Hirano, Ryosuke Kaneko, ShunsukeToyoda, Yoshimi Kawamura, Masumi Hirabayashi, Takahiro Hirabayashi, Takeshi Yagi
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 31885-31895
  • Peer Reviewed
• [Journal Article] Electrophysiological characteristics of inhibitory neurons of the prepositus hypoglossi nucleus as analyzed in Venus-expressing transgenic ratsregions (2011)
  • Author(s): Masato Shino, Ryosuke Kaneko (co-first author), Yuchio Yanagawa, Yasuo Kawaguchi, Yasuhiko Saito
  • Journal Title: Neuroscience Volume: 197 Pages: 89-98
  • Peer Reviewed
• [Journal Article] Identification of the Cluster Control Region for the Protocadherin-β Genes Located beyond the Protocadherin-γ Cluster (2011)
  • Author(s): Shinnichi Yokota, Teruyoshi Hirayama, Keizo Hirano, Ryosuke Kaneko, Shunsuke Toyoda, Yoshimi Kawamura, Masumi Hirabayashi, Takahiro Hirabayashi, Takeshi Yagi
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 31885-31895
  • Peer Reviewed
• [Presentation] regulatory changes in Protocadherin-a cluster by gene duplication (2011)
  • Author(s): Ryosuke Kaneko, Manabu Abe, Takahiro Hirabayashi, Arikuni Uchimura, Kenji Sakimura, Yuchio Yanagawa, Takeshi YagiGene
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Gene regulatory changes in Protocadherin-α cluster by gene duplication (2011)
  • Author(s): Ryosuke Kaneko, Manabu Abe, Takahiro Hirabayashi, Arikuni Uchimura, Kenji Sakimura, Yuchio Yanagawa, Takeshi Yagi
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] The generation and analysis of cerebellar purkinje cell-specific VGAT knockout mice (2011)
  • Author(s): Toshikazu Kakizaki, Mikiko Kayakabe, Ryosuke Kaneko, Hiromitsu Saito, Noboru Suzuki, Yuchio Yanagawa
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] Differential regulation between duplicated genes in Protocadherin-a cluster acquired without subsequent mutations (2011)
  • Author(s): Ryosuke Kaneko, Manabu Abe, Takahiro Hirabayashi, Arikuni Uchimura, Kenji Sakimura, Yuchio Yanagawa, Takeshi Yagi
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-15
• [Presentation] Unique gene regulation of clustered Protocadherins to confer neuronal diversity (2011)
  • Author(s): Ryosuke Kaneko
  • Organizer: 国際神経科学会 23rd Biennial Meeting of the ISN-ESN(招待講演)2011
  • Place of Presentation: Megaronアテネ国際会議場(ギリシャ)
  • Year and Date: 2011-08-30
• [Presentation] Unique gene regulation of clustered Protocadherins to confer neuronal diversity (2011)
  • Author(s): Ryosuke Kaneko
  • Organizer: 国際神経科学会23rd Biennial Meeting of the ISN-ESN
  • Place of Presentation: Megaronアテネ国際会議場(ギリシャ)(招待講演)
  • Year and Date: 2011-08-30
• [Presentation] Gene duplication influences expression and DNA methylation in Protocadherin-a cluster Keystone Meeting (2011)
  • Author(s): Ryosuke Kaneko, Yuchio Yanagawa, Takeshi Yagi
  • Organizer: Evolutionary Developmental Biology
  • Place of Presentation: Granlibakken Resort. Tahoe City, California. USA
  • Year and Date: 2011-03-01
• [Presentation] Gene duplication influences expression and DNA methylation in Protocadherin-a cluster (2011)
  • Author(s): Ryosuke Kaneko, Yuchio Yanagawa, Takeshi Yagi
  • Organizer: Keystone Meeting "Evolutionary Developmental Biology"
  • Place of Presentation: Granlibakken Resort・Tahoe City, California・USA
  • Year and Date: 2011-03-01
• [Presentation] Gene duplication alters expression and DNA methylation in Protocadherin-a clusterBMB2010 (2010)
  • Author(s): 金子涼輔, 阿部学, 平林敬浩, 内村有邦, 崎村建司, 柳川右千夫, 八木健
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Gene duplication alters expression and DNA methylation in Protocadherin-a cluster (2010)
  • Author(s): 金子涼輔、阿部学、平林敬浩、内村有邦、崎村建司、柳川右千夫、八木健
  • Organizer: BMB2010 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Gene duplication alters expression and DNA methylation in Protocadherin-a cluster (2010)
  • Author(s): 金子涼輔、阿部学、平林敬浩、内村有邦、崎村建司、柳川右千夫、八木健
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04