Analysis of gene expression changes associated with Drosophila olfactory memory formation

• Project/Area Number: 22700327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: MURAKAMI Satoshi 東京大学, 分子細胞生物学研究所, 助教 (10463902)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: (A)分子・細胞神経科学 / 嗅覚忌避記憶 / 遺伝子変動 / ショウジョウバエ / 嗅覚記憶 / 遺伝子発現変動

Research Abstract

To obtain novel insights on molecular mechanisms underlying the olfactory memory formation, we performed RNA-seq analysis in a restricted Drosophila brain region, the mushroom body(MB), which is a central neuropil for the olfactory memory. The RNA-seq analysis gave a list of genes strongly expressed in the MB and a list of gene expression changes after the olfactory learning. The following Q-PCR analysis revealed the enrichment of prospero in the adult MB. RNAi-mediated knockdown of prospero in the MB resulted in impaired memory formation. Co-expression of wild-type prospero rescued this phenotype, suggesting that MB-expressing prospero plays a critical role in the memory formation. I also found very specific expression of rgk1 in the adult MB. Rgk1 encodes a REM family protein, which is reported to regulate voltage-gated calcium channel in rodents. Since prospero and Rgk1 has mammalian homologs, detailed analysis of these genes will lead to the identification of novel and conserved molecular mechanisms underlying the memory formation.

Research Products

• [Journal Article] Optimizing Drosophila Olfactory Learning with a Semi-automated Training Device. (2010)
  • Author(s): Murakami, S., Dan, C., Zagaeski, B., Maeyama, Y., Kunes, S., Tabata, T
  • Journal Title: Journal of Neuroscience Methods Volume: 188 Pages: 195-204
  • Peer Reviewed
• [Presentation] Olfactory memory mutant analysis based on RNA-seq analysis of the Drosophila mushroom body (2012)
  • Author(s): Satoshi Murakami, Maki Otsubo, Katsuhiko Shirahige, Tetsuya Tabata
  • Organizer: 第4回グローバルCOEリトリート
  • Place of Presentation: 山梨
• [Presentation] Olfactory memory mutant analysis based on RNA-seq analysis of the Drosophila mushroom body (2012)
  • Author(s): 村上智史
  • Organizer: 第4回グローバルCOEリトリード
  • Place of Presentation: 山梨
• [Presentation] Olfactory memory mutant analysis based on RNA-seq analysis of the Drosophila mushroom body (2011)
  • Author(s): Satoshi Murakami, Maki Otsubo, Katsuhiko Shirahige, Yutaka Suzuki, Tetsuya Tabata
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 兵庫
  • Year and Date: 2011-12-13
• [Presentation] Learning-induced neural plasticity in Drosophila at a single cell level (2011)
  • Author(s): Makoto Hiroi, Daisuke Yamazaki, Satoshi Murakami, Tetsuya Tabata
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 兵庫
  • Year and Date: 2011-12-13
• [Presentation] Genes activated after the olfactory aversive conditioning in Drosophila mushroom body (2011)
  • Author(s): Satoshi Murakami, Maki Minami, Tetsuya Tabata
  • Organizer: 第4回グローバルCOEリトリート
  • Place of Presentation: 山梨
• [Presentation] RNA-seq analysis of Drosophila mushroom body transcripts and the mutant analysis with olfactory aversive learning paradigm (2011)
  • Author(s): 村上智史、大坪真樹、白髭克彦、多羽田哲也
  • Organizer: 新学術領域班会議「神経系の動作原理を明らかにするためのシステム分子行動学」
  • Place of Presentation: 大阪
• [Presentation] Olfactory memory mutant analysis based on RNA-seq analysis of the Drosophila mushroom body (2011)
  • Author(s): 村上智史、大坪真樹、鈴木穣、白髭克彦、多羽田哲也
  • Organizer: 新学術領域研究『生命科学系3分野支援活動』ゲノム支援拡大班会議
  • Place of Presentation: 大阪
• [Presentation] Olfactory memory mutant analysis based on RNA-seq analysis of the Drosophila mushroom body (2011)
  • Author(s): 村上智史
  • Organizer: 第34回日本分子生物学会年会、兵庫
  • Place of Presentation: 兵庫
• [Presentation] Learning-induced neural plasticity in Drosophila at a single cell level (2011)
  • Author(s): 多羽田哲也
  • Organizer: 第34回日本分子生物学会年会、兵庫
  • Place of Presentation: 兵庫
• [Presentation] Genes activated after the olfactory aversive conditioning in Drosophila mushroom body. (2011)
  • Author(s): Satoshi Murakami
  • Organizer: 第4回グローバルCOE全体リトリート
  • Place of Presentation: 八ヶ岳ロイヤルホテル
• [Presentation] Transcriptome analysis of olfactory aversive memory in Drosophila mushroom body (2010)
  • Author(s): Satoshi Murakami, Maki Minami, Yuko Maeyama, Yutaka Suzuki, Tetsuya Tabata
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 兵庫
  • Year and Date: 2010-12-07
• [Presentation] Identification of mushroombody-associated neurons that regulate the formation of Drosophila olfactory aversive memory (2010)
  • Author(s): Satoshi Murakami, Kohei Sekiguchi, Kazumichi Shimzu, Yuko Maeyama, Tetsuya Tabata
  • Organizer: 新学術領域班会議「神経系の動作原理を明らかにするためのシステム分子行動学」
  • Place of Presentation: 東京
• [Presentation] Mechanisms underlying Drosophila memory formation. (2010)
  • Author(s): Tetsuya Tabata, Satoshi Murakami
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Transcriptome analysis of olfactory aversive memory in Drosophila mushroom body. (2010)
  • Author(s): Satoshi Murakami
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド