Regulation of neuronal function by intracellular protein trafficking

• Project/Area Number: 22700343
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: MATSUDA Shinji 慶應義塾大学, 医学部, 講師 (60321816)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: AMPA受容体 / stargazin / アダプタータンパク質 / 長期抑圧 / リン酸化 / PIP5K_Y661 / NMDA受容体 / エンドサイトーシス / LTD / PIP5K / TARP / AP-2 / AP-3 / シナプス可塑性 / AP-3A

Research Abstract

NMDA receptor activation leads to endocytosis of AMPA receptors. Although this process controls long-term depression(LTD) induction, how it is regulated by neuronal activities is not completely clear. We showed that the NMDA receptor activation controls AMPA receptor endocytosis and LTD induction by regulating PIP5K_Y661 activity and the interaction between stargazin and Adaptor Protein Complexes.

Research Products

• [Journal Article] NMDA Receptor-Mediated PIP5K Activation to Produce PI(4,5)P_2 Is Essential for AMPA Receptor Endocytosis during LTD (2012)
  • Author(s): Takamitsu Unoki
  • Journal Title: Neuron Volume: 73 Issue: 1 Pages: 135-148
  • DOI: 10.1016/j.neuron.2011.09.034
  • Peer Reviewed
• [Journal Article] NMDA receptor-mediated PIP5K activation to produce PI(4, 5) P is essential for AMPA receptor endocytosis during LTD (2011)
  • Author(s): Unoki T, Matsuda S, Kakegawa W, Van N. T, Kohda K, Suzuki A, Funakoshi Y, Hasegawa H, Yuzaki M, Kanaho Y
  • Journal Title: These authors contributed equally Neuron Volume: 73 Pages: 35-148
  • Peer Reviewed
• [Journal Article] Cerebellar long-term depression requires dephosphorylation of TARP in Purkinje cells (2011)
  • Author(s): Nomura T, Kakegawa W, Matsuda S, Kohda K, Nishiyama J, Takahashi T, Yuzaki M
  • Journal Title: European Journal of Neuroscience Volume: 35 Pages: 402-410
  • Peer Reviewed
• [Journal Article] D-Serine regulates cerebellar LTD and motor coordination through the δ2 glutamate receptor (2011)
  • Author(s): Kakegawa W, Miyoshi Y, Hamase K, Matsuda S, Matsuda K, Kohda K, Emi K, Motohashi J, Konno R, Zaitsu K, Yuzaki M
  • Journal Title: Nature Neuroscience Volume: (印刷中)
  • Peer Reviewed
• [Presentation] 小脳長期抑制のTARPによる制御 (2010)
  • Author(s): 野村寿博, 掛川渉, 松田信爾, 幸田和久, 柚崎通介
  • Organizer: 第33回日本神経科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] Cerebellar LTD and regulation by TARPs (2010)
  • Author(s): Nomura T, Kakegawa W, Matsuda S, Kohda K, Yuzaki M
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] New mechanisms regulating stability and dynamics of AMPA receptors (2010)
  • Author(s): Yuzaki M, Matsuda S
  • Organizer: 日本神経科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02