The functional analysis of a novel ubiquitin like posttranslational modifier UBL3

• Project/Area Number: 22700346
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Fujita Health University
• Principal Investigator: AGETA Hiroshi 藤田保健衛生大学, 総合医科学研究所, 助教 (40416649)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子・細胞神経科学 / 新規翻訳後修飾 / 翻訳後修飾 / ユビキチン様タンパク / UBL3 / UBL3ノックアウトマウス / 膜局在 / UBL3過剰発現マウス / 神経細胞 / 軸索伸長 / バイオインフォマティクス

Research Abstract

By using a bioinformatical method, we identified UBL3 as a highly conserved posttranslational modifier. We found that UBL3 were localized at the plasma membrane and attached to other proteins like an ubiquitin protein. However, the functional role of UBL3 is still unknown. To elucidate roles of UBL3, we searched for interaction protein with UBL3.As a result, we identified several UBL3-binding proteins. One UBL3-binding protein enhanced UBL3ylation which is a posttranslational modification via UBL3.These findings suggest that UBL3 may play a pivotal role as an essential component of a novel membrane localization system.

Research Products

• [Journal Article] Multifunctional roles of activins in the brain. (2011)
  • Author(s): Hiroshi Ageta, Kunihiro Tsuchida.
  • Journal Title: Vitamins and Hormones Volume: 85 Pages: 185-206
  • Peer Reviewed
• [Journal Article] Activin plays a key role in the maintenance of long-term memory and late-LTP (2010)
  • Author(s): Hiroshi Ageta, Shiro Ikegami, Masami Miura, Masao Masuda, Rika Migishima Toshiaki Hino, Noriko Takashima, Akiko Murayama, Hiromu Sugino, Mitsutoshi Setou, Satoshi Kida, Minesuke Yokoyama, Yoshihisa Hasegawa, Kunihiro Tsuchida, Toshihiko Aosaki, Kaoru Inokuchi
  • Journal Title: Learning and Memory Volume: 17 Pages: 168-175
  • Peer Reviewed
• [Presentation] 変形性関節症の軟骨細胞に対する網羅的プロテオミクス解析 (2012)
  • Author(s): 池田大樹、上田洋司、土田邦博、山田治基
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 愛知県
  • Year and Date: 2012-03-10
• [Presentation] 変形性関節症の軟骨細胞に対する網羅的プロテオミクス解析 (2012)
  • Author(s): 池田大樹、上田洋司、土田邦博、山田治基
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: ウィルあいち(愛知県)
  • Year and Date: 2012-03-10
• [Presentation] Proteomics analysis of new animal models of bipolar disorder (2011)
  • Author(s): Hiroshi Ageta, Akihiko Takasaki, Kaoru Inokuchi, Kunihiro Tsuchida
  • Organizer: 北米神経科学大会
  • Place of Presentation: ワシントンDC
  • Year and Date: 2011-11-14
• [Presentation] Proteomics analysis of new animal models of bipolar disorder (2011)
  • Author(s): Hiroshi Ageta, Akihiko Takasaki, Kaoru Inokuchi, Kunihiro Tsuchida
  • Organizer: 北米神経科学大会
  • Place of Presentation: コンベンションセンター(ワシントンDC)
  • Year and Date: 2011-11-14
• [Presentation] Proteomics analysis of new animal models of bipolar disorder (2010)
  • Author(s): Hiroshi Ageta, Kaoru Inokuchi, Kunihiro Tsuchida
  • Organizer: 日本神経科学学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Book] The ubiquitin proteasome system in nervous system : from physiology to pathology. (2010)
  • Author(s): Ikuko Yao, Hiroshi Ageta, Mitsutoshi Setou
  • Total Pages: 19
  • Publisher: NOVA Publishers
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/ICMS/res04.html