Analyses of mechanism for neuronal circuit refinement in barrel cortex by mouse genetics and electroporation method
| Project/Area Number |
22700347
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neuroscience in general
|
|---|
| Research Institution | National Institute of Genetics |
|---|
Principal Investigator |
MIZUNO Hidenobu 国立遺伝学研究所, 個体遺伝研究系, 助教 (00567159)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 大脳皮質 / マウス遺伝学 / 電気穿孔法 / 蛍光蛋白質 / 2光子イメージング / Cre/loxPシステム / NMDA型グルタミン酸受容体 / バレル / 遺伝学 / 神経科学 / バレル皮質 / in vivoイメージング / 2光子顕微鏡 / NMDA受容体 / ノックアウト |
|---|
| Research Abstract |
In this study, we investigated the role of NMDAR-mediated neuronal activity in the refinement of neuronal connections in the mouse barrel cortex. By developing novel sparse neuron labeling system, we found that NMDAR plays an important role for the dendrite refinement of barrel neurons during neonatal stages when barrel formation is in progress. We also developed a method for in vivo 2-photon imaging of neonatal mice brain, and observed the dendritic morphology of developing barrel cells.
|
Report
(3 results)
Research Products
(5 results)
