| Project/Area Number |
22700374
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Gunma University |
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Principal Investigator |
IIZUKA Akira 群馬大学, 大学院・医学系研究科, 助教 (10466683)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 遺伝子治療 / プルキンエ細胞 / レンチウイルス / 神経可塑性 / 小脳発達 / 回路形成 / 小脳 / レンチウイルスベクター |
|---|
| Research Abstract |
Lentiviral vectors expressing RORα were injected into the cerebellar cortex of P6, P13 or P20 staggerer mice, and the effects were studied 3 weeks after the injection. Staggerer mice lentivirally treated at P6 showed marked dendritic differentiation of PCs and removal CF synapses from PCs, whereas those lentivirally treated at P13 displayed only a partial rescue of dendritic differentiation and failure of removal of CF synapses from PCs. No rescue was observed in staggerer mice lentivirally treated at P20. Together with evidence that protein expression in the brain starts about 5 days after lentiviral vector injection, these results suggest that staggerer mutant cerebellum maintains potential to respond to RORα at 2nd postnatal week, but loses almost completely at 4th postnatal week.
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