Analysis of the neuropathological mechanism by using calpain mutant mice
Project/Area Number |
22700379
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
TAKANO Jiro 独立行政法人理化学研究所, 神経蛋白制御研究チーム, 研究員 (60415213)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | カルパイン / プロテアーゼ / カルシウムシグナル / 遺伝子改変マウス / 神経変性疾患 / アルツハイマー病 / 学習記憶 |
Research Abstract |
In this study, we have analyzed the motor abnoromality in calpain-1 and calpain-2 double knockout mice. Neuron-specific deleted knockout showed abnormalities in motor and/or visual capacity in Morris water maze test. Then, we identified several substrates of calpain-1 and calpain-2, which relate the functional abnormalities, by masspectrometic technique and the iTRAQ method. However, some of the substrates were not cleaved so much by calpain. Thus, we are trying to identify calpain substrates in cell culture system using calcium ionophores.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3).2013
Author(s)
Hubener J, Weber JJ, Richter C, Honold L, Weiss A, Murad F, Breuer P, Wullner U, Bellstedt P, Paquet-Durand F, Takano J, Saido TC, Riess O, Nguyen HP.
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Journal Title
Hum Mol Genet.
Volume: 22(3)
Pages: 508-18
Related Report
Peer Reviewed
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[Journal Article] Metabolic stress response implicated in diabetic retinopathy: the role of calpain, and the therapeutic impact of calpain inhibitor.2012
Author(s)
Shanab AY, Nakazawa T, Ryu M, Tanaka Y, Himori N, Taguchi K, Yasuda M, Watanabe R, Takano J, Saido T, Minegishi N, Miyata T, Abe T, Yamamoto M.
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Journal Title
Neurobiol Dis.
Volume: 48(3)
Pages: 556-67
Related Report
Peer Reviewed
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[Journal Article] Mechanistic involvement of the calpain-calpastatin system in Alzheimer neuropathology.2012
Author(s)
Higuchi M, Iwata N, Matsuba Y, Takano J, Suemoto T, Maeda J, Ji B, Ono M, Staufenbiel M, Suhara T, Saido TC.
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Journal Title
FASEB J.
Volume: 26(3)
Pages: 1204-17
Related Report
Peer Reviewed
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[Journal Article] Critical role of calpain in axonal damage-induced retinal ganglion cell death.2012
Author(s)
Ryu M, Yasuda M, Shi D, Shanab AY, Watanabe R, Himori N, Omodaka K, Yokoyama Y, Takano J, Saido T, Nakazawa T.
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Journal Title
J Neurosci Res.
Volume: 90(4)
Pages: 802-15
Related Report
Peer Reviewed
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[Journal Article] Mechanistic involvement of the calpain-calpastatin system in Alzheimer neuropathology2012
Author(s)
Makoto Higuchi, Nobuhisa Iwata, Yukio Matsuba, Jiro Takano, Takahiro Suemoto, Jun Maeda, Bin Ji, Maiko Ono, Matthias Staufenbiel, Tetsuya Suhara, Takaomi C.Saido
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Journal Title
The FASEB Journal
Volume: (In press)
Related Report
Peer Reviewed
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[Journal Article] Potent amyloidogenicity and pathogenicity of Aβ43.2011
Author(s)
Saito T, Suemoto T, Brouwers N, Sleegers K, Funamoto S, Mihira N, Matsuba Y, Yamada K, Nilsson P, Takano J, Nishimura M, Iwata N, Van Broeckhoven C, Ihara Y, Saido TC.
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Journal Title
Nat Neurosci.
Volume: 14(8)
Pages: 1023-32
NAID
Related Report
Peer Reviewed
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[Journal Article] Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25.2011
Author(s)
Sato K, Minegishi S, Takano J, Plattner F, Saito T, Asada A, Kawahara H, Iwata N, Saido TC, Hisanaga S.
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Journal Title
J Neurochem.
Volume: 117(3)
Pages: 504-15
Related Report
Peer Reviewed
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[Journal Article] Potent amyloidogenicity and pathogenicity of Aβ432011
Author(s)
Takashi Saito, Takahiro Suemoto, Nathalie Brouwers, Kristel Sleegers, Satoru Funamoto, Naomi Mihira, Yukio Matsuba, Kazuyuki Yamada, Per Nilsson, Jiro Takano, Masaki Nishimura, Nobuhisa Iwata, Christine Van Broeckhoven, Yasuo Ihara, Takaomi C Saido
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Journal Title
Nature Neuroscience
Volume: 14
Issue: 8
Pages: 1023-1032
DOI
NAID
Related Report
Peer Reviewed
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