Visualization of pathogenic mechanism of parkinson's disease with Keima, the novel autophagy probe.

• Project/Area Number: 22700381
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KATAYAMA Hiroyuki 独立行政法人理化学研究所, 細胞機能探索技術開発チーム, 研究員 (00415126)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: オートファジー / パーキンソン病 / ミトコンドリア / マイトファジー / イメージング / 蛍光タンパク質 / Keima、Parkin / 脳神経疾患 / Keima / Parkin

Research Abstract

We produced Keima, a novel autophagy probe. Because the signal of Keima remains and accumulates after termination of autophagy, this probe has high sensitivity and can determines total amount of autophagy. While this probe can detect not only macroautophagy, but also other autophagy. mt-mKeima, a mitochondria targeting construct, can detect mitophagy. This probe has been distributed and used in some dozen laboratory.

Research Products

• [Journal Article] A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery. (2011)
  • Author(s): Hiroyuki Katayama, Takako Kogure, Noboru Mizushima, Tamotsu Yoshimori, Atsushi Miyawaki
  • Journal Title: Chem. & Biol. Volume: 18 (8) Pages: 1042-1052
  • Peer Reviewed
• [Journal Article] A Sensitive and Quantitative Technique for Detecting Autophagic Events Based on Lysosomal Delivery (2011)
  • Author(s): Katayama, et al
  • Journal Title: Chemistry & Biology Volume: 18 Issue: 8 Pages: 1042-1052
  • DOI: 10.1016/j.chembiol.2011.05.013
  • Peer Reviewed
• [Presentation] Keima, a lysosomal proteases-resistant and pH-sensitive GFP-like protein, enables the visualization of autophagy. (2010)
  • Author(s): 片山博幸(発表者)、宮脇敦史、小暮貴子、吉森保、水島昇、山本章嗣
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: グランキューブ大阪
• [Presentation] Keima, a lysosomal proteases-resistant and pH-sensitive GFP-like protein, enables the visualization of autophagy. (2010)
  • Author(s): 片山博幸
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪府大阪市
• [Remarks]
  • URL: http://www.jst.go.jp/pr/announce/20110826/
• [Remarks]
  • URL: http://www.jst.go.jp/pr/announce/20110826/index.html