Functional analysis of novel NYAP family proteins

• Project/Area Number: 22700391
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: YOKOYAMA Kazumasa 東京大学, 医科学研究所, 助教 (20431760)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 神経細胞 / チロシンキナーゼ / PI3K / WAVE / シグナル伝達 / 神経科学

Research Abstract

The phosphoinositide 3-kinase(PI3K) pathway has been extensively studied in neuronal function and morphogenesis. However, the precise molecular mechanisms of PI3K activation and its downstream signalling in neurons remain elusive. In this study, we demonstrated that the NYAPs bridge a PI3K-WAVE1 association by simultaneously interacting with PI3K and the WAVE1 complex. Disruption of the NYAP genes in mice affects brain size and neurite elongation.

Research Products

• [Journal Article] Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression (2012)
  • Author(s): Takahashi A, et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 419 Issue: 2 Pages: 268-273
  • DOI: 10.1016/j.bbrc.2012.02.007
  • Peer Reviewed
• [Journal Article] Activation of Receptor Protein-Tyrosine Kinases from the Cytoplasmic Compartment (2012)
  • Author(s): Yamanashi Y, Tezuka T, and Yokoyama K.
  • Journal Title: J Biochem Volume: (n press) Issue: 4 Pages: 353-359
  • DOI: 10.1093/jb/mvs013
  • NAID: 10031166074
  • Peer Reviewed
• [Journal Article] NYAP : a phosphoprotein family that links PI3K to WAVE1 signalling in neurons (2011)
  • Author(s): K.Yokoyama
  • Journal Title: EMBO J Volume: 30 Issue: 23 Pages: 4739-4754
  • DOI: 10.1038/emboj.2011.348
  • Peer Reviewed
• [Journal Article] Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development (2011)
  • Author(s): Chen C, et al
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 411 Issue: 2 Pages: 360-364
  • DOI: 10.1016/j.bbrc.2011.06.148
  • Peer Reviewed
• [Journal Article] The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability (2011)
  • Author(s): Ito K, et al
  • Journal Title: Protein Cell Volume: 2 Issue: 4 Pages: 755-763
  • DOI: 10.1111/j.1365-2443.2011.01492.x
  • Peer Reviewed
• [Journal Article] CNOT2-depletion disrupts and inhibits the CCR4-NOT deadenylase complex and induces apoptotic cell death. (2011)
  • Author(s): Ito K, et al.
  • Journal Title: Genes Cells Volume: (掲載確定 in press)
  • Peer Reviewed
• [Journal Article] NMDAR2B tyrosine phosphorylation modulates anxiety-like behavior by regulating CRF expression (2010)
  • Author(s): Delawary M, Tezuka T, Kiyama Y, Yokoyama K, Inoue T, Hattori S, Hashimoto R, Umemori H, Manabe T, Yamamoto T, Nakazawa T
  • Journal Title: Mol. Brain Volume: 3 Issue: 1 Pages: 37-37
  • DOI: 10.1186/1756-6606-3-37
• [Journal Article] NMDAR2B tyrosine phosphorylation regulates anxiety-like behavior and CRF expression in the amygdala. (2010)
  • Author(s): Delawary M, et al.
  • Journal Title: Mol.Brain Volume: 3 Pages: 37-37
  • Peer Reviewed
• [Presentation] NYAPファミリーはContactinファミリー下流においてPI3K-Akt経路を活性化し神経突起伸長を促進する (2010)
  • Author(s): 横山一剛,山本雅
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 新規NYAPファミリーはContactinファミリー下流においてPI3K-Akt経路を活性化し神経突起伸長を促進する (2010)
  • Author(s): 横山一剛, 山本雅
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2010-12-10
• [Presentation] Novel Phosphoprotein Family Activating the PI3K-Rac1-WAVE1 Signaling in Developing Neurons (2010)
  • Author(s): Yokoyama K, and Yamamoto T.
  • Organizer: Salk institute meeting(the 2010 Protein
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-08-18
• [Presentation] NYAP : the Nbvel Phosphoprotein Family Activating the PI3K-Rac1-WAVE1 Signaling in Developing Neurons. (2010)
  • Author(s): Yokoyama K, Yamamoto T
  • Organizer: Salk institute meeting (the 2010 Protein Phosphorylation and Cell Signaling)
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-08-18
• [Book] 実験医学増刊 (2012)
  • Author(s): 横山一剛,手塚徹,山梨裕司
  • Publisher: 羊土社
• [Book] シグナル伝達研究最前線2012,実験医学増刊Vol.30 No.5 (2012)
  • Author(s): 横山一剛, 手塚徹, 山梨裕司
  • Publisher: 羊土社
• [Remarks]
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• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/frontier/
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/wakate/profile/ika_yokoyama.html
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/frontier/index.html