Analyses of N-terminal processing of secretory lectin PAP-III in neuronal regeneration

• Project/Area Number: 22700398
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University (2011); Osaka City University (2010)
• Principal Investigator: KONISHI Hiroyuki 名古屋大学, 大学院・医学系研究科, 助教 (90448746)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: グリア細胞 / 神経再生 / 再生医学 / 神経科学 / 脳・神経 / 細胞・組織 / 糖鎖

Research Abstract

I revealed that N-terminal processed PAP-III(ΔN-PAP-III) formed fibrillar structures and attached tightly to neuronal surfaces. When a dense networks ofΔN-PAP-III fibers were prepared on the culture dishes, neurites preferentially adhere to theΔN-PAP-III fibers and neurite extension was enhanced. These results suggest a possibility that PAP-III may form insoluble fibrillar structures around injury site and would provide regenerating axons with a platform for elongation.

Research Products

• [Journal Article] Analysis on expression of the regenerating gene(Reg) family members Reg-IIIβand Reg-IIIγin the mouse during deve lopment (2012)
  • Author(s): Matsumoto S, Konishi H, Maeda R, Kiryu-Seo S, Kiyama H.
  • Journal Title: J Comp Neuro Volume: 520(3) Pages: 479-94
  • Peer Reviewed
• [Journal Article] Expression analysis of the regenerating gene (Reg) family members Reg-IIIβand Reg-IIIγin the mouse during development (2012)
  • Author(s): Matsumoto S, Konishi H, Maeda R, Kiryu-Seo S, Kiyama H
  • Journal Title: J Comp Nuerol Volume: 520 Issue: 3 Pages: 479-494
  • DOI: 10.1002/cne.22705
  • Peer Reviewed
• [Journal Article] Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland (2011)
  • Author(s): Konishi H, Ogawa T, Kawahara S, Matsumoto S, Kiyama H.
  • Journal Title: Biochem Biophys Res Commun Volume: 407(1) Pages: 7-12
  • Peer Reviewed
• [Journal Article] Pancreatitis-associated protein-I and pancreatitis-associated protein-III expression in a rat model of kainic acid-induced seizure (2011)
  • Author(s): Kawahara S, Konishi H, Morino M, Ohata K, Kiyama H.
  • Journal Title: Neuroscienc Volume: 175 Pages: 273-80
  • Peer Reviewed
• [Journal Article] Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland (2011)
  • Author(s): 小西博之、小川登紀子、川原慎一、松本早紀子、木山博資
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 407(1) Issue: 1 Pages: 7-12
  • DOI: 10.1016/j.bbrc.2011.02.064
  • Peer Reviewed
• [Journal Article] Local ventilation system successfully reduced formaldehyde exposure during gross anatomy dissection classes (2010)
  • Author(s): Takahashi M, Abe M, Yamagishi T, Nakatani K, Okade T, Ogawa T, Konishi H, Kiryu-Seo S, Kiyama H, Nakajima Y.
  • Journal Title: Anat Sci Int Volume: 85(4) Pages: 251-2
  • NAID: 10031162230
  • Peer Reviewed
• [Journal Article] Id1, Id2 and Id3 are induced in rat melanotrophs of the pituitary gland by dopamine suppression under continuousstress (2010)
  • Author(s): Konishi H, Ogawa T, Nakagomi S, Inoue K, Tohyama M, Kiyama H.
  • Journal Title: Neuroscienc Volume: 169(4) Pages: 1527-34
  • Peer Reviewed
• [Journal Article] Id1, Id2 and Id3 are induced in rat melanotrophs of the pituitary gland by dopamine suppression under continuous stress (2010)
  • Author(s): 小西博之
  • Journal Title: Neuroscience Volume: 169(4) Pages: 1527-34
  • Peer Reviewed
• [Journal Article] Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland
  • Author(s): 小西博之
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: (印刷中)
  • Peer Reviewed
• [Presentation] PAP-III(Reg-IIIgamma)はN末端切断により線維状構造を形成し軸索伸長の足場となる (2011)
  • Author(s): 小西博之、松本早紀子、木山博資
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光(金沢)
  • Year and Date: 2011-09-28
• [Presentation] PAP-III (Reg-IIIgamma)はN末端切断により線維状構造を形成し軸索伸長の足場となる (2011)
  • Author(s): 小西博之、松本早紀子、木山博資
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 瑠璃光(金沢)
  • Year and Date: 2011-09-28
• [Presentation] PAP-IIIはN末端切断により線維状構造を形成し軸索伸長の足場となる (2011)
  • Author(s): 小西博之、松本早紀子、木山博資
  • Organizer: 第2回名古屋大学医学部・生理学研究所合同シンポジウム
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2011-08-20
• [Presentation] PAP-III(Reg-2)はN末端切断により線維状構造を形成し軸索伸長の足場となる (2011)
  • Author(s): 小西博之、松本早紀子、川原慎一、前田理亜、木山博資
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜コンベンションセンター(横浜)
  • Year and Date: 2011-03-30
• [Presentation] Thepancreatit is-associated protein-III(PAP-III) is polymerized into a fibrillarstructure by the cleavage of its N-terminus (2010)
  • Author(s): Konishi H, Matsumoto S, Kawahara S, Maeda R, Kiyama H
  • Organizer: Society for Neuroscience 40thAnnual Meeting
  • Place of Presentation: San Diego Convention Center(San Diego), USA
  • Year and Date: 2010-11-13
• [Presentation] The pancreatitis-associated protein-III (PAP-III) is polymerized into a fibrillar structure by the cleavage of its N-terminus (2010)
  • Author(s): 小西博之
  • Organizer: Society for Neuroscience 40th Annual Meeting
  • Place of Presentation: San Diego Convention Center (米国サンディエゴ)
  • Year and Date: 2010-11-13
• [Presentation] PAP-IIIはN末端切断により線維状構造を形成する (2010)
  • Author(s): 小西博之、松本早紀子、木山博資
  • Organizer: 第12回ORIGIN夏のワークショプ
  • Place of Presentation: レイクフォレストリゾート(京都)
  • Year and Date: 2010-09-05
• [Presentation] Pancreatitis-associated protein-III(PAP-III)はN末端切断により線維状構造を形成する (2010)
  • Author(s): 小西博之、川原慎一、松本早紀子、前田理亜、木山博資
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会・合同大会
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2010-09-03
• [Presentation] Pancreatitis-associated protein-III (PAP-III)はN末端切断により線維状構造を形成する (2010)
  • Author(s): 小西博之
  • Organizer: 第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会・合同大会
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2010-09-03
• [Remarks] ホームページ
  • URL: http://www.med.nagoya-u.ac.jp/Anatomy2/contact.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/Anatomy2/contact.html