Quantitative analysis of molecular dynamics in the transduction cascade using caged compounds and imaging techniques
| Project/Area Number |
22700414
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 感覚神経細胞 / 情報変換 / 電気生理学 / パッチクランプ法 / ケージド化合物 / 神経生理学 / シグナルトランスダクション / イオンチャネル / ナノ繊毛 / 可視化 / 電気生理 / 神経生理 / 嗅覚 / UVレーザー / セカンドメッセンジャー |
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| Research Abstract |
Olfactory transduction cascade starts from the receptor protein which binds to odorant molecules. After the binding, G-protein and AC are activated continuously. It leads to open the transduction channels (CNG, Cl(Ca)) and generate current responses, after [cAMP]i and [Ca2+]i increased. Spatial and temporal distributions of cytoplasmic elements (cAMP, Ca2+) are key factors in the olfactory transduction cascade.We examined that based on the olfactory adaptation and non-linear signal amplificationwith these techniques. 1) Local laser light stimulation (LMS-ROI). 2) Current responses with caged-cAMP photolysis (patch-clamp) and 3) Ca-imaging(Fluo4) in parallel.
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Report
(4 results)
Research Products
(39 results)
