| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Olfactory transduction cascade starts from the receptor protein which binds to odorant molecules. After the binding, G-protein and AC are activated continuously. It leads to open the transduction channels (CNG, Cl(Ca)) and generate current responses, after [cAMP]i and [Ca2+]i increased. Spatial and temporal distributions of cytoplasmic elements (cAMP, Ca2+) are key factors in the olfactory transduction cascade.We examined that based on the olfactory adaptation and non-linear signal amplificationwith these techniques. 1) Local laser light stimulation (LMS-ROI). 2) Current responses with caged-cAMP photolysis (patch-clamp) and 3) Ca-imaging(Fluo4) in parallel.
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