Development ofa new probe for dynamic imaging of transplanted cells without surgical operation.

• Project/Area Number: 22700417
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Jikei University School of Medicine (2012); Tokyo Medical and Dental University (2010-2011)
• Principal Investigator: HARA Chikako 東京慈恵会医科大学, 医学部, 助教 (40528452)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経発生 / 神経再生 / イメージング技術開発 / 発光イメージング / in vivo イメージング / MRI / in vivoイメージング / 蛍光 / 発光 / フェリチン / イメージング / ルシフェラーゼ / プローブ開発

Research Abstract

A new probe which detectable for fluorescence, chemiluminescence and magnetic resonance (MR) imaging was constructed and evaluated in this study. The fusion of a fluorescent protein to a chemiluminescent protein and ferritin as iron indicator has enabled to pursue the transplanted cells at in vivo. Since the novel probe which possess three kinds of function have worked well, we are preparing the new publication of the novel in vivoimaging method using Venus of fluorescent protein as expression marker, luciferase as live cell marker and ferritin protein as MRI marker.

Research Products

• [Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior (2012)
  • Author(s): Hara-Miyauchi C., Tsuji O., Hanyu A., Okada S, Yasuda A., Fukano T., Akazawa C., Nakamura M., Imamura T., Matsuzaki Y., Okano H.J., Miyawaki A., Okano H.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 419 Pages: 188-193
• [Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior (2012)
  • Author(s): Hara-Miyauchi C, Tsuji O, Hanyu A, Okada S, Yasuda A, Fukano T, Akazawa C, Nakamura M, Imamura T, Matsuzaki Y, Okano HJ, Miyawaki A, Okano H
  • Journal Title: Biochem Biophys Res Commun Volume: 419(2) Issue: 2 Pages: 188-93
  • DOI: 10.1016/j.bbrc.2012.01.141
  • Peer Reviewed
• [Journal Article] Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production. (2011)
  • Author(s): Fukuda H., Mochizuki S., Abe H., Okano H.J., Hara-Miyauchi C., Okano H., Yamaguchi N., Nakayama M., D'Armiento J., Okada Y.
  • Journal Title: British Journal of Cancer. Volume: 105 Pages: 1615-1624
• [Journal Article] Comparative Study of Methods for Administering Neural Stem/Progenitor Cells to Treat Spinal Cord Injury in Mice (2011)
  • Author(s): Takahashi Y., Tsuji O., Kumagai G., Hara M. C., Okano J. H., Miyawaki A., Toyama Y., Okano H., Nakamura M.
  • Journal Title: Cell Transplantation. Volume: 20 Pages: 727-739
• [Presentation] 生細胞発光イメージングの in vivo 解析への応用 (2013)
  • Author(s): 原 央子
  • Organizer: 生理学東京談話会
  • Place of Presentation: タワーホール船橋
  • Year and Date: 2013-03-26
• [Presentation] 生細胞発光イメージングの in vivo 解析への応用
  • Author(s): 原 央子
  • Organizer: 生理学東京談話会
  • Place of Presentation: タワーホール船橋
• [Remarks] (1)化学工業新聞2012年2月29日にフォルティシモルシフェラーゼの研究成果が掲載された。記事題名「蛍光と発光機能兼備 薬剤投与研究など威力」
• [Remarks] (2)科学新聞2012年3月9日にフォルティシモルシフェラーゼの研究成果が掲載された。記事題名「新しい蛍光蛋白質開発 微細構造を可視化」
• [Remarks] (3)Yahooニュースとマイナビニュース(インターネット)に2012年2月23日から、フォルティシモルシフェラーゼの研究成果が掲載された。記事題名「慶応大、蛍光と発光の利点を併せ持つバイオイメージング技術を開発」