Development ofa new probe for dynamic imaging of transplanted cells without surgical operation.
Project/Area Number |
22700417
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Neurophysiology and muscle physiology
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Research Institution | Jikei University School of Medicine (2012) Tokyo Medical and Dental University (2010-2011) |
Principal Investigator |
HARA Chikako 東京慈恵会医科大学, 医学部, 助教 (40528452)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 神経発生 / 神経再生 / イメージング技術開発 / 発光イメージング / in vivo イメージング / MRI / in vivoイメージング / 蛍光 / 発光 / フェリチン / イメージング / ルシフェラーゼ / プローブ開発 |
Research Abstract |
A new probe which detectable for fluorescence, chemiluminescence and magnetic resonance (MR) imaging was constructed and evaluated in this study. The fusion of a fluorescent protein to a chemiluminescent protein and ferritin as iron indicator has enabled to pursue the transplanted cells at in vivo. Since the novel probe which possess three kinds of function have worked well, we are preparing the new publication of the novel in vivoimaging method using Venus of fluorescent protein as expression marker, luciferase as live cell marker and ferritin protein as MRI marker.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior2012
Author(s)
Hara-Miyauchi C., Tsuji O., Hanyu A., Okada S, Yasuda A., Fukano T., Akazawa C., Nakamura M., Imamura T., Matsuzaki Y., Okano H.J., Miyawaki A., Okano H.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 419
Pages: 188-193
Related Report
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[Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior2012
Author(s)
Hara-Miyauchi C, Tsuji O, Hanyu A, Okada S, Yasuda A, Fukano T, Akazawa C, Nakamura M, Imamura T, Matsuzaki Y, Okano HJ, Miyawaki A, Okano H
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Journal Title
Biochem Biophys Res Commun
Volume: 419(2)
Issue: 2
Pages: 188-93
DOI
Related Report
Peer Reviewed
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