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Functional analysis of calcineurin, a sleep-related gene in the fruit fly, Drosophila melanogaster

Research Project

Project/Area Number 22700433
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Fusional basic brain science
Research InstitutionKumamoto University

Principal Investigator

TOMITA Jun  熊本大学, 発生医学研究所, 特定事業研究員 (40432231)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsショウジョウバエ / 睡眠 / カルシニューリン / ドーパミン / 学習記憶 / 記憶 / 脳
Research Abstract

We recently reported that calcineurin(Ca^<2+>/calmodulin-dependent protein phosphatase) is an important regulator of sleep in Drosophila(Tomita et al., 2011). The pan-neuronal knockdown of Calcineurin A at 14F(CanA-14F), which is one of the three calcineurin catalytic A subunit genes in Drosophila, resulted in sleep loss, while an adult-specific misexpression of a constitutively active form of CanA-14F in all neurons increased sleep. We identified brain neurons important for the function of CanA-14F in sleep regulation by targeted CanA-14F knockdown using the GAL4-UAS system. Moreover, we found that knockdown of D1 dopamine receptor in these neurons significantly increased sleep. These results suggest a cross-talk between Ca^<2+>-calcineurin and dopaminergic signaling pathways in Drosophila sleep regulation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Remarks (2 results)

  • [Journal Article] Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster2012

    • Author(s)
      T. Ueno, J. Tomita, S. Kume, K. Kume
    • Journal Title

      PLoS ONE

      Volume: 7(2)

    • NAID

      120004997257

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] High calorie diet augments age-associated sleep impairment in Drosophila2012

    • Author(s)
      M. Yamazaki, J. Tomita, K. Takahama, T. Ueno, M. Mitsuyoshi, E. Sakamoto, S. Kume, K. Kume
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 417 Pages: 812-816

    • NAID

      120004161565

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pan-neuronal knockdown of the c-Jun N-terminal Kinase(JNK) results in a reduction in sleep and longevity in Drosophila2012

    • Author(s)
      K. Takahama, J. Tomita, T. Ueno, M. Yamazaki, S. Kume, K. Kume
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 417 Pages: 807-811

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pan-neuronal knockdown of the c-JunN-terminal Kinase (JNK) results in areduction in sleep and longevity inDrosophila2012

    • Author(s)
      K. Takahama, J. Tomita, T. Ueno, M.Yamazaki, S. Kume, K. Kume
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 417 Issue: 2 Pages: 807-11

    • DOI

      10.1016/j.bbrc.2011.12.040

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High calorie diet augmentsage-associated sleep impairment inDrosophila2012

    • Author(s)
      M. Yamazaki, J. Tomita, K. Takahama,T. Ueno, M. Mitsuyoshi, E. Sakamoto, S.Kume, K. Kume
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 417 Issue: 2 Pages: 812-6

    • DOI

      10.1016/j.bbrc.2011.12.041

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dopamine modulates metabolic rate andtemperature sensitivity in Drosophila melanogaster2012

    • Author(s)
      T. Ueno, J. Tomita, S. Kume, K. Kume
    • Journal Title

      PLoS One

      Volume: 7 Issue: 2 Pages: e31513-e31513

    • DOI

      10.1371/journal.pone.0031513

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster2011

    • Author(s)
      J. Tomita, M. Mitsuyoshi, T. Ueno, Y. Aso, H. Tanimoto, Y. Nakai, T. Aigaki, S. Kume, K. Kume
    • Journal Title

      J. Neurosci

      Volume: 31 Pages: 13137-13146

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pan-Neuronal Knockdown of CalcineurinReduces Sleep in the Fruit Fly,Drosophila melanogaster2011

    • Author(s)
      J. Tomita, M. Mitsuyoshi, T. Ueno, Y.Aso, H. Tanimoto, Y. Nakai, T. Aigaki, S.Kume, K. Kume
    • Journal Title

      J Neurosci

      Volume: 31 Issue: 37 Pages: 13137-46

    • DOI

      10.1523/jneurosci.5860-10.2011

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Calcineurin regulates sleep and memory in Drosophila2012

    • Author(s)
      J. Tomita
    • Organizer
      International Symposium Frontiers in Behavioral Brain Science
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2012-03-20
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Calcineurin regulates sleep and memory in Drosophila2011

    • Author(s)
      J. Tomita
    • Organizer
      International Symposium "Designing the Circadian Clock"
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-11-25
    • Related Report
      2011 Final Research Report
  • [Presentation] カルシニューリンによるショウジョウバエの睡眠-覚醒制御2011

    • Author(s)
      冨田淳
    • Organizer
      第18回日本時間生物学会学術大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-11-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Calcineurin regulates sleep and memory in Drosophila2011

    • Author(s)
      J. Tomita
    • Organizer
      KEY Forum in Developmental Biology and Regenerative Medicine
    • Place of Presentation
      熊本大学
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Calcineurin Regulates Sleep and Memory in Drosophila2010

    • Author(s)
      J. Tomita
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸コンベンションセンター
    • Year and Date
      2010-09-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Calcineurin Regulates Sleep and Memory in Drosophila.2010

    • Author(s)
      Jun Tomita
    • Organizer
      Neuro 2010
    • Place of Presentation
      神戸コンベンションセンター(神戸市)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np50.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.imeg.kumamoto-u.ac.jp/newpress/np50.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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