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Development of highly effective gene knock-in procedure using neural crest-specific gene expression machinery

Research Project

Project/Area Number 22700449
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

SATO Takahiro  京都大学, iPS細胞研究所, 特任研究員 (10572000)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発生工学
Research Abstract

To identify the neural crest cell-specific enhancer region as well as transcription factors that bind to the enhancer region, we conducted luciferase assay using several upstream promoter regions on endothelin type-A receptor gene locus. Consequently, we found that about 30 bases as a critical enhancer sequence that consisted of presumable tandem binding sites of two transcription factors. In vivo experiments in transgenic mice exhibited an insufficiency of this enhancer sequence for neural crest cell-specific forced expression of LacZ gene. These results suggest that a possible coordination with other region may be required to proper gene expression in neural crest cells.
Adenoviral transduction in fertilized eggs exhibited toxicity for their later normal development, whereas strong expression of LacZ was observed. Therefore, the important issue of viral toxicity remained to be overcome.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Remarks (1 results)

  • [Journal Article] Identification and developmental analysis of endothelin type-A expressing cells in the mouse kidney2011

    • Author(s)
      Kitazawa T, Sato T, Nishiyama K, Asai R, Arima Y, Uchijima Y, Kurihara Y, Kurihara H
    • Journal Title

      Gene Expr Patterns

      Volume: 11 Pages: 371-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and developmental analysis of endothelin receptor type-A expressing cells in the mouse kidney2011

    • Author(s)
      Kitazawa T, Sato T, Nishiyama K, Asai R, Arima Y, Uchijima Y, Kurihara Y, Kurihara H.
    • Journal Title

      Gene Expr. Patterns

      Volume: 11 Issue: 7 Pages: 371-377

    • DOI

      10.1016/j.gep.2011.04.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endothelin receptor type-A expression defines a distinct cardiac subdomain within the heart field, with a later implication of this signaling pathway in chamber myocardium formation2010

    • Author(s)
      Asai R, Kurihara Y, Fujisawa K, Sato T, Kawamura Y, Kokubo H, Tonami K, Nishiyama K, Uchijima Y, Miyagawa-Tomita S, Kurihara H.
    • Journal Title

      Development

      Volume: 137 Issue: 22 Pages: 3823-3833

    • DOI

      10.1242/dev.054015

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endothelin receptor type A expression defines a distinctcardiac subdomain within the heart field and is later implicated in chamber myocardium formation.2010

    • Author(s)
      Asai R, Kurihara Y, Fujisawa K, Sato T, Kawamura Y, Kokubo H, Tonami K, Nishiyama K, Uchijima Y, Miyagawa-Tomita S, Kurihara H.
    • Journal Title

      Development

      Volume: 137 Pages: 3823-3833

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Hox gene Function and possible crosstalk with Dlx genes in cranofacial morphogenesis2011

    • Author(s)
      Kitazawa T, Fujisawa K, Kurihara Y, Arima Y, Kawamura Y, Sato T, Uchijima Y, Kurihara H
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      Yohohama
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Hox gene Function and possible crosstalk with Dlx genes in cranofacial morphogenesis2011

    • Author(s)
      Kitazawa T, Fujisawa K, Kurihara Y, Arima Y, Kawamura Y, Sato T, Uchijima Y, Kurihara H
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Endothelin-1/Endothelin type-A receptor signaling regulates pharyngeal arch artery development through Dlx5/6-independent pathway2011

    • Author(s)
      Kim KS, Arima Y, Asai R, Sato T, Nishiyama K, Kurihara H
    • Organizer
      The 19th Annual Meeting of the Japanese Vascular Biology and Medicine Organization/The 1st Asia-Pscific Vascular Biology Meeting
    • Place of Presentation
      Tokyo
    • Year and Date
      2011-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Endothelin-1/Endothelin type-A receptor signaling regulates pharyngeal arch artery development through Dlx5/6-independent pathway2011

    • Author(s)
      Kim KS, Arima Y, Asai R, Sato T, Nishiyama K, Kurihara H
    • Organizer
      The 19th Annual Meeting of the Japanese Vascular Biology and Medicine Organization The 1st Asia-Pscific Vascular Biology Meeting
    • Place of Presentation
      Tokyo Station Conference
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Endothelin type-A receptor expression defines a distinct subpoplation within the first heart field contributing to chamber myocardium2010

    • Author(s)
      Asai R, Kurihara Y, Fujisawa K, Sato T, Kawamura Y, Kokubo H, Tonami K, Nishiyama K, Uchijima Y, Miyagawa-Tomita S, Kurihara H
    • Organizer
      BMB2010
    • Place of Presentation
      Kobe
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Endothelin type-A receptor expression defines a distinct subpoplation within the first heart field contributing to chamber myocardium2010

    • Author(s)
      Asai R, Kurihara Y, Fujisawa K, Sato T, Kawamura Y, Kokubo H, Tonami K, Nishiyama K, Uchijima Y, Miyagawa-Tomita S, Kurihara H.
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Involvment of non-coding RNA in the Endothelin signaling in branchal arch development2010

    • Author(s)
      Kurihara Y, Uchijima Y, Fujisawa K, Sato T, Kushiyama S, Asai R, Kitazawa T, Nishiyama K, Kurihara H
    • Organizer
      BMB2010
    • Place of Presentation
      Kobe
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Transcription regulation of non-coding RNA Evf-2 in the Endothelin-1/Dlx5/Dlx6 pathway2010

    • Author(s)
      Uchijima Y, Kurihara Y, Sato T, Fujisawa K, Kushiyama S, Kurihara H
    • Organizer
      BMB2010
    • Place of Presentation
      Kobe
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Transcription regulation of non-coding RNA Evf-2 in the Endothelin-1/Dlx5/Dlx6 pathway2010

    • Author(s)
      Uchijima Y, Kurihara Y, Sato T, Fujisawa K, Kushiyama S, Kurihara H.
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Involvment of non-coding RNA in the Endothelin signaling in branchal arch development2010

    • Author(s)
      Kurihara Y, Uchijima Y, Fujisawa K, Sato T, Kushiyama S, Asai R, Kitazawa T, Nishiyama K, Kurihara H.
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Involvement of the endothelin-1/endothelin receptor type-A signaling in determination of the mandibular identity in mouse craniofacial development2010

    • Author(s)
      Sato T, Kurihara Y, Asai R, Kawamura Y, Tonami K, Uchijima Y, Heude E, Ekker M, Levi G, Kurihara H
    • Organizer
      Gordon Research Conrference : Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Lucca(Barga), Italy
    • Related Report
      2011 Final Research Report
  • [Presentation] Non-coding RNA regulation of endothelin signaling in branchal arch development2010

    • Author(s)
      Kurihara Y, Uchijima Y, Fujisawa K, Sato T, Kushiyama S, Asai R, Kitazawa T, Nishiyama K, Kurihara H.
    • Organizer
      Gordon Research Conrference : Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Lucca(Barga), Italy
    • Related Report
      2011 Final Research Report
  • [Presentation] Involvement of the endothelin-1/endothelin receptortype-A signaling in determination of the mandibular identity in mouse craniofacial development2010

    • Author(s)
      Sato T, Kurihara Y, Asai R, Kawamura Y, Tonami K, UchijimaY, Heude E, Ekker M, Levi G, Kurihara H.
    • Organizer
      Gordon Research Conrference : Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Lucca (Barga), Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] Non-coding RNA regulation of endothelin signaling in branchal arch development2010

    • Author(s)
      Kurihara Y, Uchijima Y, Fujisawa K, Sato T, Kushiyama S, Asai R, Kitazawa T, Nishiyama K, Kurihara H.
    • Organizer
      Gordon Research Conrference : Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Lucca (Barga), Italy
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.cira.kyoto-u.ac.jp/i/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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