Analyses of mutant mice lacking histone modification factors.

• Project/Area Number: 22700451
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Kanazawa University
• Principal Investigator: NARUSE Chie 金沢大学, 学際科学実験センター, 助教 (30372486)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 発生・分化 / 生殖細胞 / マウス / ヒストン修飾 / 遺伝子改変動物 / 始原生殖細胞

Research Abstract

We generated HP1 gamma mutant mice and found that histone methylation at pericentromeric heterochromatin was reduced in the mutant germ cells at meiosis. Next, we found that HP1 gamma is essential for cell cycle progression of primordial germ cells(PGC). Moreover, we found that histone demethylase regulates expression of Hox genes, and the demethylase-deficient mice died perinatally.

Research Products

• [Journal Article] HP1γ links histone methylation marks to meiotic synapsis in mice (2011)
  • Author(s): Takada Y, Naruse C, Costa Y, Shirakawa T, Tachibana M, Sharif J, Kezuka-Shiotani F, Kakiuchi D, Masmnoto H, Shinkai Y, Ohbo K, Peters AH, Turner JM, Asano M, Koseki H
  • Journal Title: Development Volume: 138 Issue: 19 Pages: 4207-4217
  • DOI: 10.1242/dev.064444
  • Peer Reviewed
• [Journal Article] Loss of hererochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell-cycle progression in mice (2011)
  • Author(s): Abe, K., et al
  • Journal Title: Biology of Reproduction Volume: 85 Issue: 5 Pages: 1013-1024
  • DOI: 10.1095/biolreprod.111.091512
  • Peer Reviewed
• [Journal Article] β4-Galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development (2010)
  • Author(s): Nishie, T., Hikimochi, Y., Zama, K., Fukusumi, Y., Ito, M., Yokoyama, H., Naruse, C., Ito, M. and Asano, M.
  • Journal Title: Glycobiology Volume: 20 Issue: 10 Pages: 1311-1322
  • DOI: 10.1093/glycob/cwq098
  • Peer Reviewed
• [Journal Article] Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. (2010)
  • Author(s): Toshikazu Nishie, et al.
  • Journal Title: Glycobiology Volume: 20 Pages: 1311-1322
  • Peer Reviewed
• [Presentation] マウスKdm6bの欠損はホメオティックトランスフォーメーションを引き起こす (2011)
  • Author(s): 成瀬智恵、柴田進和、阿部可奈恵、川口隆之、浅野雅秀
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2011-12-13
• [Presentation] マウスKdm6bの欠損はホメオティックトランスフォーメーションを引き起こす (2011)
  • Author(s): 成瀬智恵, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] HP1γ欠損によるPGCの減少は細胞周期進行の異常による (2011)
  • Author(s): 阿部可奈恵、成瀬智恵、加藤智明、西内巧、斎藤通紀、浅野雅秀
  • Organizer: 第4回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府豊中市)
  • Year and Date: 2011-11-17
• [Presentation] HP1γ欠損によるPGCの減少は細胞周期進行の異常によるポスター (2011)
  • Author(s): 阿部可奈恵、成瀬智恵、斎藤通紀、浅野雅秀
  • Organizer: 生殖サイクル若手勉強会
  • Place of Presentation: 大阪アカデミア(大阪府大阪市)
  • Year and Date: 2011-07-15
• [Presentation] Kdm6b欠損マウスの解析 (2011)
  • Author(s): 成瀬智恵、柴田進和、阿部可奈恵、川口隆之、浅野雅秀
  • Organizer: 生殖サイクル若手勉強会
  • Place of Presentation: 大阪アカデミア(大阪府大阪市)
  • Year and Date: 2011-07-14
• [Presentation] HP1γ変異マウスにおける細胞周期異常による始原生殖細胞(PGC)の減少 (2011)
  • Author(s): 阿部可奈恵, ら
  • Organizer: 第58回日本実験動物学会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2011-05-26
• [Presentation] Heterochromatin protein 1γis essential for primordial germ cells proliferation」poster presentation (2011)
  • Author(s): Kanae Abe, Chie Naruse, Tomoaki Kato, Takumi Nishiuchi, Mitinori Saitou, Masahide Asano
  • Organizer: 分子生物学会第11回シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県金沢市)
  • Year and Date: 2011-05-25
• [Presentation] Heterochromatin protein 1γ is essential for primordial germ cells proliferation (2011)
  • Author(s): Kanae Abe, ら
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県立音楽堂(石川県)
  • Year and Date: 2011-05-25
• [Presentation] β4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development (2010)
  • Author(s): Toshikazu Nishie, Yoko Hikimochi, Kota Zama, Yoshiyasu Fukusumi, Mitutoshi Ito, Haruka Yokoyama, Chie Naruse, Makoto Ito, and Masahide Asano
  • Organizer: BMB2010
  • Place of Presentation: Kobe Portisland(Kobe Convention Center, Kobe, Hyogo)
  • Year and Date: 2010-12-10
• [Presentation] The role of heterochromatin protein 1(HP1)γin primordial germ cells (2010)
  • Author(s): Kanae Abe, Chie Naruse, Tomoaki Kato, Takumi Nishiuchi, Mitinori Saitou, Masahide Asano
  • Organizer: Genetics & Genomics meeting 2010
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
  • Year and Date: 2010-10-27
• [Presentation] The role of heterochromatin protein 1 (HP1)Y in primordial germ cells. (2010)
  • Author(s): Kanae Abe, et al.
  • Organizer: The Mouse Development, Genetics & Genomics meeting
  • Place of Presentation: Cold Spring Harbor Laboratory (USA)
  • Year and Date: 2010-10-27
• [Presentation] 始原生殖細胞の発生と分化におけるHP1γの機能解析 (2010)
  • Author(s): 阿部可奈恵、成瀬智恵、斎藤通紀、浅野雅秀
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール(鳥取県米子市)
  • Year and Date: 2010-05-28
• [Presentation] ヒト15番染色体を保持したトランスクロモソミックマウスの作出 (2010)
  • Author(s): 目黒-堀家牧子、宮野勝、杉原一司、成瀬智恵、久郷裕之、押村光雄、浅野雅秀、堀家慎一
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール(鳥取県米子市)
  • Year and Date: 2010-05-28
• [Presentation] 始原生殖細胞の発生と分化におけるHP1γの機能解析 (2010)
  • Author(s): 阿部可奈恵, ら
  • Organizer: 第4回日本エピジェネティクス研究会年会
  • Place of Presentation: 米子市文化ホール(鳥取県)
  • Year and Date: 2010-05-28
• [Presentation] ヒト15番染色体を保持したトランスクロモソミックマウスの作出 (2010)
  • Author(s): 目黒-堀家牧子, ら
  • Organizer: 第4回日本エピジェネティクス研究会
  • Place of Presentation: 米子市文化ホール(鳥取県)
  • Year and Date: 2010-05-28
• [Remarks]
  • URL: http://kiea.w3.kanazawa-u.ac.jp/tglab/tganimHP
• [Remarks]
  • URL: http://asrc.w3.kanazawa-u.ac.jp/
• [Remarks]
  • URL: http://web.kanazawa-u.ac.jp/~asrc/index.html