Exploration of disease-related proteins from spontaneous model of melanoma by proteomics analysis

• Project/Area Number: 22700457
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Chubu University
• Principal Investigator: KAWAMOTO Yoshiyuki 中部大学, 生命健康科学部, 准教授 (10410664)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 疾患モデル / プロテオミクス解析 / メラノーマ / バイオマーカー / RET / 転移 / 血清プロテオーム / 悪性黒色腫

Research Abstract

RET-transgenic (RET-Tg) mice are useful for identifying melanoma-related biomarkers which can predict tumor progression or metastasis. In this study, we found some serum proteins which may relate to melanoma progression or metastasis by proteomic study. In addition, invasive activity of Mel-ret cells, which were established from RET-tg mice, treated with TNF-alpha were enhanced, and some up- or down-regulated genes were identified by DNA array analysis. 2-Dimensional Fluorescence Difference Gel Electrophoresis (2D-DIGE) analysis revealed differential expression of some proteins between B16 melanoma cell line and the highly metastatic cell line B16-F10. In conclusion, we identified some potential candidate for melanoma-related biomarkers.

Research Products

• [Journal Article] RAS/RAF/MEK/ERK and PI3K/PTEN/AKT Signaling in Malignant Melanoma Progression and Therapy. (2012)
  • Author(s): Ya manoshita O Kato M
  • Journal Title: Dermatol Res Pract. Volume: 2012 Pages: 354191-354191
  • DOI: 10.1155/2012/354191
  • Peer Reviewed
• [Journal Article] Molecular Network Associated with MITF in Skin Melanoma Development and Progression. (2011)
  • Author(s): Ya manoshita O Kato M
  • Journal Title: J Skin Cancer. Volume: 2011 Pages: 730170-730170
  • DOI: 10.1155/2011/730170
  • Peer Reviewed
• [Journal Article] Control of genetically prescribed protein tyrosine kinase activities by environment-linked redox reactions (2011)
  • Author(s): I. Nakashima, Y. Kawamoto, K. Takeda, M.Kato
  • Journal Title: Enzyme research Volume: 2011 Pages: 896567-896567
  • Peer Reviewed
• [Journal Article] Protective role of Gipie,a Girdin family protein,in endoplasmic reticulum stress responses in endothelial cells (2011)
  • Author(s): E. Matsushita, N. Asai, A. Enomoto, Y.Kawamoto, T. Kato, S. Mii, K. Maeda, R. Shibata, S. Hattori, M. Hagikura, K. Takahashi, M. Sokabe, Y. Murakumo, T. Murohara, M. Takahashi
  • Journal Title: Molecular biology of the cell Volume: 22 Pages: 736-747
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21289099
  • Peer Reviewed
• [Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury (2011)
  • Author(s): A. Furukawa, Y. Kawamoto, Y. Chiba, S.Takei, S. Hasegawa-Ishii, N. Kawamura, K. Yoshikawa, M. Hosokawa, S. Oikawa, M. Kato, A. Shimada
  • Journal Title: Neurobiology of disease Volume: 43 Issue: 3 Pages: 706-714
  • DOI: 10.1016/j.nbd.2011.05.024
  • Peer Reviewed
• [Journal Article] Protective Role of Gipie, a Girdin Family Protein, in Endoplasmic Reticulum Stress Responses in Endothelial Cells (2011)
  • Author(s): Matsushita E., et al
  • Journal Title: Mol Biol Cell Volume: 22 Issue: 6 Pages: 736-747
  • DOI: 10.1091/mbc.e10-08-0724
  • Peer Reviewed
• [Journal Article] A redox-linked novel pathway for arsenic-mediated RET tyrosine kinase activation (2010)
  • Author(s): M. Kato, K. Takeda, K. Hossain, N.D.Thang, Y. Kaneko, M. Kumasaka, O. Yamanoshita, N. Uemura, M. Takahashi, N. Ohgami, Y. Kawamoto
  • Journal Title: Journal of cellular biochemistry Volume: 110 Issue: 2 Pages: 399-407
  • DOI: 10.1002/jcb.22550
  • Peer Reviewed
• [Journal Article] A redox-linked novel pathway for arsenic-mediated RET tyrosine kinase activation. (2010)
  • Author(s): Kato M., et al.
  • Journal Title: J Cell Biochem Volume: 110 Pages: 399-407
  • Peer Reviewed
• [Presentation] メラノーマ自然発症RETトランスジェニックマウスを用いた腫瘍関連バイオマーカーの探索 (2011)
  • Author(s): 川本善之
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国際会議場(京都)
  • Year and Date: 2011-09-22