| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
RET-transgenic (RET-Tg) mice are useful for identifying melanoma-related biomarkers which can predict tumor progression or metastasis. In this study, we found some serum proteins which may relate to melanoma progression or metastasis by proteomic study. In addition, invasive activity of Mel-ret cells, which were established from RET-tg mice, treated with TNF-alpha were enhanced, and some up- or down-regulated genes were identified by DNA array analysis. 2-Dimensional Fluorescence Difference Gel Electrophoresis (2D-DIGE) analysis revealed differential expression of some proteins between B16 melanoma cell line and the highly metastatic cell line B16-F10. In conclusion, we identified some potential candidate for melanoma-related biomarkers.
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