Development of human antibody producing mice with human immune systems
| Project/Area Number |
22700458
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
ITO Ryoji 公益財団法人実験動物中央研究所, 免疫研究室, 研究員 (60425436)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ヒト化マウス / 疾患モデル / ヒト抗体 / アレルギー / ヒト抗体産生 / ヒト遺伝子導入NOGマウス / ヒト型抗体 |
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| Research Abstract |
The purpose in this research is producing humanized mice reconstituted with human immune system. We obtained 2 strains of human gene transgenic (Tg) mice (HLA-DR4 Tg, IL-3/GM-CSF Tg), and human HSCs were transferred into them to analyze human immune responses in vivo. HLA-DR4 Tg mice showed the production of an antigen specific human IgG antibody and promotion of T cell activity. Meanwhile, differentiation of human mast cells and basophils were increased in hIL-3/GM Tg mice, and they could induce passive allergic response in vivo. Therefore, these humanized Tg mice may contribute to medical research such as the pre-clinical study on the development of new drug.
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Report
(4 results)
Research Products
(20 results)
