Characterization of the Lethal Canine Distemper Virus Outbreak in Cynomolgus Monkeys

• Project/Area Number: 22700459
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SAKAI Kouji 国立感染症研究所, ウイルス第三部, 研究員 (70515535)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ウイルス / 感染症 / 実験動物学 / 獣医学 / イヌジステンパーウイルス / 霊長類 / サル / SLAM / nectin4 / 動物

Research Abstract

In 2008 in Japan, a Canine distemper virus (CDV) outbreak also occurred in cynomolgus monkeys imported from China. In that outbreak, forty-six monkeys died from severe pneumonia during a quarantine period. A CDV strain (CYN07-dV) was isolated in Vero cells expressing dog signaling lymphocyte activation molecule (SLAM). Phylogenic analysis showed that CYN07-dV was closely related to the recent CDV outbreaks in China, suggesting continuing chains of CDV transmission in monkeys. In vitro, CYN07-dV uses macaca SLAM and macaca nectin4 as receptors as efficiently as dog SLAM and dog nectin4, respectively. CYN07-dV showed high virulence in experimentally infected cynomolgus monkeys and excreted progeny viruses in oral fluid and feces. These data revealed that some of the CDV strains, like CYN07-dV, have the potential to cause severe disease in monkeys and spread among them.

Research Products

• [Journal Article] Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. (2013)
  • Author(s): Sakai K, Nagata N, Ami Y, Seki F, Suzaki Y, Iwata-Yoshikawa N, Suzuki T, Fukushi S, Mizutani T, Yoshikawa T, Otsuki N, Kurane I, Komase K, Yamaguchi R, Hasegawa H, Saijo M, Takeda M, Morikawa S
  • Journal Title: Journal of Virology Volume: 87 Pages: 1105-1114
• [Journal Article] Lethal canine distemper virus outbreak in cynomolgus monkeys in Japan in 2008. (2013)
  • Author(s): Sakai K, Nagata N, Ami Y, Seki F, Suzaki Y, Iwata-Yoshikawa N, Suzuki T, Fukushi S, Mizutani T, Yoshikawa T, Otsuki N, Kurane I, Komase K, Yamaguchi R, Hasegawa H, Saijo M, Takeda M, Morikawa S.
  • Journal Title: J. Virol. Volume: 87 Issue: 2 Pages: 1105-1114
  • DOI: 10.1128/jvi.02419-12
  • Peer Reviewed
• [Presentation] 犬ジステンパーウイルスの霊長類レセプターの利用について (2013)
  • Author(s): 酒井宏治
  • Organizer: 2nd Negative Strand Virus-Japan Symposium
  • Place of Presentation: 沖縄
• [Presentation] Canine distemper virus intrinsically uses monkey receptors and readily adapts to use human receptors as well. (2013)
  • Author(s): Kouji Sakai, Fumio Seki, Maino Tahara, Noriyuki Otsuki, Yasushi Ami, Masayuki Saijo, Ryoji Yamaguchi, Katsuhiro Komase, Makoto Takeda and Shigeru Morikawa
  • Organizer: 15th International Conference on Emerging Infectious Diseases (EID) in the Pacific Rim
  • Place of Presentation: Singapore
• [Presentation] ジステンパーウイルス(CDV)のレセプター指向性の解析:CDV はヒトへの脅威となり得るのか? (2012)
  • Author(s): 酒井宏治、關文緒、田原舞乃、大槻紀之、山 口良二、伊藤由梨、福原秀雄、前仲勝実、駒瀬 勝啓、西條政幸、森川茂、竹田誠
  • Organizer: 1^<st> Negative Strand Virus-Japan Symposium
  • Place of Presentation: 長崎
• [Presentation] カニクイザルで致死的感染症を起こしたジステンパーウイルスのサルSLAM ならびにNectin4の効率的な利用 (2012)
  • Author(s): 酒井宏治、關文緒、網康至、福士秀悦、西條政幸、森川茂、山口良二、駒瀬勝啓、竹田誠
  • Organizer: 第154回日本獣医学会学術集会、DV-13
  • Place of Presentation: 盛岡
• [Presentation] カニクイザルで致死的感染症を起こしたジステンパーウイルスのサルレセプターの効率的な利用:ジステンパーウイルスはヒトへの脅威となり得るのか? (2012)
  • Author(s): 酒井宏治、關文緒、網康至、田原舞乃、中津 祐一郎、大槻紀之、福原秀雄、福士秀悦、吉河 智城、西條政幸、森川茂、前仲勝実、山口良二、 駒瀬勝啓、竹田誠
  • Organizer: 第60回日本ウイルス学会学術集会、O3-C-11
  • Place of Presentation: 大阪
• [Presentation] カニクイザルで致死的感染症を起こしたジステンパーウイルスのサルレセプターの効率的な利用：ジステンパーウイルスはヒトへの脅威となり得るのか？ (2012)
  • Author(s): 酒井宏治
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] カニクイザルで致死的感染症を起こしたジステンパーウイルスのサルSLAMならびにNectin4の効率的な利用 (2012)
  • Author(s): 酒井宏治
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
• [Presentation] カニクイザルで致死的感染症を起こしたイヌジステンパーウイルス(CDV)のレセプター指向性の解析:CDVはヒトへの脅威となり得るのか? (2012)
  • Author(s): 酒井宏治
  • Organizer: カニクイザルで致死的感染症を起こしたイヌジステンパーウイルス(CDV)のレセプター指向性の解析:CDVはヒトへの脅威となり得るのか?
  • Place of Presentation: 長崎
• [Presentation] Virulence of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan. (2011)
  • Author(s): Kouji Sakai, Noriyo Nagata, Yasushi Ami, Katsuhiro Komase, Makoto Takeda, Masayuki Saijo, Shigeru Morikawa
  • Organizer: 2011 MEASLES VIRUS MINI-SYMPOSIUM, 39
  • Place of Presentation: Rochester, MN, USA
  • Year and Date: 2011-07-15
• [Presentation] Virulence of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan (2011)
  • Author(s): Kouji Sakai
  • Organizer: 2011 MEASLES VIRUS MINI-SYMPOSIUM
  • Place of Presentation: Rochester, MN, USA
  • Year and Date: 2011-07-15
• [Presentation] Virological and pathological analyses of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan. (2011)
  • Author(s): Kouji Sakai, Noriyo Nagata, Yasushi Ami, Katsuhiro Komase, Makoto Takeda, Masayuki Saijo, Shigeru Morikawa
  • Organizer: American Society for Virology 30th Annual Meeting, W8-12
  • Place of Presentation: Minnesota, MN, USA
• [Presentation] Characterization of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan. (2011)
  • Author(s): Kouji Sakai, Yohei Nishio, Noriyo Nagata, Yasushi Ami, Katsuhiro Komase, Masayuki Shimojima, Ken Maeda, Makoto Takeda, Masayuki Saijo, Shigeru Morikawa
  • Organizer: International Union of Microbiological Societies 2011 Congress, VI-PO22-6
  • Place of Presentation: Sapporo, Japan.
• [Presentation] Virological and pathological analyses of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan (2011)
  • Author(s): Kouji Sakai
  • Organizer: American Society for Virology 30th Annual Meeting
  • Place of Presentation: Minnesota, MN, USA
• [Presentation] Characterization of canine distemper virus isolated from cynomolgus monkeys during 2008 epizootic in Japan (2011)
  • Author(s): Kouji Sakai
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] カニクイザルで致死的感染症を起こしたイヌジステンパーウイルスの性状と実験感染サルでの病原性の解析 (2010)
  • Author(s): 酒井宏治・永田典代、網康至、岩田奈織、鈴木忠樹、水谷哲也、福士秀悦、須崎百合子、緒 方もも子、西條政幸、長谷川秀樹、山田靖子、 倉根一郎、森川茂
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
• [Presentation] カニクイザルで致死的感染症を起こしたイヌジス テンパーウイルスのサル及びイヌでの病原性の解析 (2010)
  • Author(s): 酒井宏治、田丸精治、前田健、永田典代、網 康至、岩田奈織子、鈴木忠樹、水谷哲也、福士 秀悦、須崎百合子、緒方もも子、長谷川秀樹、 西條政幸、山田靖子、倉根一郎、森川茂
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
• [Presentation] カニクイザルで致死的感染症を起こしたイヌジステンパーウイルスの性状と実験感染サルでの病原性の解析(DV-51) (2010)
  • Author(s): 酒井宏治
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広(帯広畜産大学キャンパス)
• [Presentation] カニクイザルで致死的感染症を起こしたイヌジステンパーウイルスのサル及びイヌでの病原性の解析(P2-013) (2010)
  • Author(s): 酒井宏治
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島(あわぎんホール)
• [Remarks]
  • URL: http://idsc.nih.go.jp/iasr/29/345/kj3451.html
• [Remarks]
  • URL: http://idsc.nih.go.jp/iasr/29/345/kj3451.html