Development of activatable protein probe for detection of tumor hypoxic microenvironment
| Project/Area Number |
22700469
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | バイオイメージング / バイオセンサー / 腫瘍内低酸素微小環境 / 生体イメージング / 低酸素応答 / タンパク質分子設計 / 分子動力学シミュレーション / 腫瘍内低酸素 |
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| Research Abstract |
Tumor hypoxic microenvironment is potentially an excellent marker for malignant tumors and in vivo imaging of intratumoral hypoxia is significant for cancer treatment.To construct the novel activatable protein probe, whose signals can be monitored only in hypoxic conditions, H-type dimer formation of fluorescent dyes was designed within the pVHL molecule to detect the structural change of pVHL. As the results, fluorescent signal of probe in native state is very low, while it was dramatically increased in denatured state, suggesting that signal intensity of the probe can be changed depending on its structure. This probe would be useful for the detection of intratumoral hypoxia.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Detection of the onset of ischemia and carcinogenesis by hypoxia-inducible transcription factor-based in vivo bioluminescence imaging2011
Author(s)
Kadonosono T, Kuchimaru T, Yamada S, Takahashi Y, Murakami A, Tani T, WatanabeH, Tanaka T, Hirota K, Inoue M, Tsukamoto T, Toyoda T, Urano K, Machida K, Eto T, Ogura T, Tsutsumi H, Ito M, Hiraoka M, Kondoh G, Kizaka-Kondoh S
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Journal Title
Related Report
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[Journal Article] Detection of the onset of ischemia and carcinogenesis by hypoxia-inducible transcription factor-based in vivo bioluminescence imaging2011
Author(s)
Kadonosono T, Kuchimaru T, Yamada S, Takahashi Y. Murakami A, Watanabe H, Tani T, Inoue M, Tsukamoto T, Toyoda T, Tanaka T, Hirota K, Urano K, Machida K, Eto T, Ogura T, Tsutsumi H, Ito M, Hiraoka M, Kondoh G, Kizaka-Kondoh S
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Journal Title
PLoS ONE
Volume: 6(11)
Issue: 11
Pages: e26640-e26640
DOI
Related Report
Peer Reviewed
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