Development of MR microscope for organogenesis research

• Project/Area Number: 22700470
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: UENO Tomohiro 京都大学, 医学(系)研究科(研究院), 助教 (10379034)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: MRI(磁気共鳴映像法) / 顕微鏡 / 磁気共鳴映像法 / MRI

Research Abstract

We developed a magnetic resonance microscope by using high magnetic field of 14.1 T. We visualized chemically-fixed medaka in 20 μm isovoxel three dimensionally, where field gradients of 4.0 T/m at maximum were applied. Comparing 3D MRimages of p53 cancer suppressor gene mutant medaka with those of wild type medaka, differences in internal organs were obtained. In addition to that, we analyzed digitized effects in quantitative susceptibility mapping, which gave us a tissue iron contentmap and could be used for detecting local iron accumulation in a brain of a Parkinson’s disease patient.

Research Products

• [Journal Article] Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Journal Title: the proceedings of 35th Annual International Conference of the IEEE Volume: (in press)
  • Peer Reviewed
• [Journal Article] Comparisons of Quantitative Susceptibility Mapping (QSM) by using restricted oversampled spatial unit dipole field (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Journal Title: the proceedings of International Society of Magnetic Resonance in Medicine Volume: vol.21 Pages: 2418-2418
  • Peer Reviewed
• [Journal Article] Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Journal Title: the proceedings of 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society Volume: 1 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Postmortem 3D Image Comparison of Wild Type and p53 Knockout Medaka by MR Microscope (2012)
  • Author(s): M.Murashima, T. Ueno, K. Sakai, Y. Taniguchi, and N. Sugimoto
  • Journal Title: Molecular Imaging and Biology Volume: vol.14, supplement 1
  • NAID: 40020110874
  • Peer Reviewed
• [Journal Article] メダカ連続標本切片からの高解像度3D画像構築及びMRM画像との比較 (2012)
  • Author(s): 庄司航,上野智弘,酒井晃二,杉本直三
  • Journal Title: 信学技報 Volume: 111巻 Pages: 47-52
  • NAID: 10031093064
• [Journal Article] Postmortem 3D Image Comparison of Wild Type and p53 Knockout Medaka by MR Microscope (2012)
  • Author(s): M.Murashima, T.Ueno, K.Sakai, Y.Taniguchi, N.Sugimoto
  • Journal Title: Molecular Imaging and Biology Volume: (in press)
  • NAID: 40020110874
  • Peer Reviewed
• [Journal Article] メダカ連続標本切片からの高解像度3D画像構築及びMRM画像との比較 (2012)
  • Author(s): 庄司航, 上野智弘, 酒井晃二, 杉本直三
  • Journal Title: 信学技報 Volume: 111 Pages: 47-52
  • NAID: 10031093064
• [Journal Article] 固定された脊椎動物モデルのNMRスペクトルとMRI画像の検討 (2011)
  • Author(s): 桑原航,上野智弘,村島舞,平石真寛,山口佳美,三浦岳, 波多野直也,谷口善仁,浦山慎一, 福山秀直,酒井晃二,杉本直三
  • Journal Title: 信学技報 Volume: 110巻 Pages: 173-178
  • NAID: 110008675424
• [Journal Article] 3D image analysis of p53 mutant medaka by the MR Microscope (2011)
  • Author(s): M.Murashima, T.Ueno, K.Sakal, N.Hatano, Y.Taniguchi, N.Sugimoto
  • Journal Title: 生物物理 Volume: 51
• [Journal Article] 固定された脊椎動物モデルのNMRスペクトルとMRI画像の検討 (2011)
  • Author(s): 桑原航, 上野智弘, 村島舞, 平石真寛, 山口佳美, 三浦岳, 波多野直也, 谷口善仁, 浦山慎一, 福山秀直, 酒井晃二, 杉本直三
  • Journal Title: 信学技報 Volume: 110 Pages: 173-178
  • NAID: 110008675424
• [Journal Article] 3D Visualization of the Medaka by MR Microscope (2010)
  • Author(s): T. Ueno, S. Kanayama, W. Kuwabara S. Urayama, N. Hatano, Y. Taniguchi, N. Sugimoto, and H. Fukuyama
  • Journal Title: the Proceedings of the 3rd Biomedical Engineering International Conference Pages: 201052-201052
  • Peer Reviewed
• [Journal Article] MRI顕微鏡によるメダカガン細胞のin situ3次元可視化に向けた固定法の検討 (2010)
  • Author(s): 桑原航,上野智弘,浦山慎一, 谷口善仁,山口佳美,三浦岳, 福山秀直,杉本直三
  • Journal Title: 生体医工学シンポジウム2010予稿集 Pages: 1-10
• [Journal Article] 3D Visualization of the Medaka by MR Microscope (2010)
  • Author(s): T.Ueno, S.Kanayama, W.Kuwabara, S.Urayama, N.Hatano, Y.Taniguchi, N.Sugimoto, H.Fukuyama
  • Journal Title: Proceedings of the 3^<rd> Biomedical Engineering International Conference Volume: (CD-ROM) Pages: 1-2
  • Peer Reviewed
• [Journal Article] MRI顕微鏡によるメダカガン細胞のin situ3次元可視化に向けた固定法の検討 (2010)
  • Author(s): 桑原航, 上野智弘, 浦山慎一, 谷口善仁, 山口佳美, 三浦岳, 福山秀直, 杉本直三
  • Journal Title: 生体医工学シンポジウム2010 予稿集 Volume: (CD-ROM) Pages: 1-10
• [Presentation] Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2013-07-04
• [Presentation] Comparisons of Quantitative Susceptibility Mapping (QSM) by using restricted oversampled spatial unit dipole field (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Organizer: 21st Annual Meeting & Exhibition of ISMRM
  • Place of Presentation: Salt Lake City, Utah, USA
  • Year and Date: 2013-04-23
• [Presentation] Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping (2013)
  • Author(s): Mai Murashima, Tomohiro Ueno, and Naozo Sugimoto
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka
• [Presentation] Quantitative Susceptibility Mapping of MR microscope (2012)
  • Author(s): Mai Murashima, Tomohiro Ueno, Koji Sakai, and NaozoSugimoto
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都府京都市
  • Year and Date: 2012-09-22
• [Presentation] In vivo imaging of small fish using magnetic resonance microscope (2012)
  • Author(s): Masahiro Hiraishi, Tomohiro Ueno, Koji Sakai, and Naozo Sugimoto
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都府京都市
  • Year and Date: 2012-09-22
• [Presentation] メダカ連続標本切片からの高解像度3D 画像構築及びMRM 画像との比較 (2012)
  • Author(s): 庄司航,上野智弘,酒井晃二,杉本直三
  • Organizer: 電子情報 通信 学会医 用画像 研究 会
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2012-01-19
• [Presentation] メダカ連続標本切片からの高解像度3D画像構築及びMRM画像との比較 (2012)
  • Author(s): 庄司航, 上野智弘, 酒井晃二, 杉本直三
  • Organizer: 電子情報通信学会医用画像研究会
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2012-01-19
• [Presentation] In vivo imaging of small fish using magnetic resonance microscope (2012)
  • Author(s): Masahiro Hiraishi, Tomohiro Ueno, Koji Sakai, and Naozo Sugimoto
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都市
• [Presentation] Quantitative Susceptibility Mapping of MR microscope (2012)
  • Author(s): Mai Murashima, Tomohiro Ueno, Koji Sakai, and Naozo Sugimoto
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都市
• [Presentation] 3D image analysis of p53 mutant medaka by the MR Microscope (2011)
  • Author(s): M.Murashima, T. Ueno, K. Sakai, N. Hatano, Y. Taniguchi, and N. Sugimoto
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県姫路市
  • Year and Date: 2011-09-17
• [Presentation] Postmortem 3D Image Comparison of Wild Type and p53 Knockout Medaka by MR Microscope (2011)
  • Author(s): M.Murashima, T. Ueno, K. Sakai, Y. Taniguchi, and N. Sugimoto
  • Organizer: World Molecular Imaging Congress 2011
  • Place of Presentation: San Diego CA, USA
  • Year and Date: 2011-09-09
• [Presentation] High resolution 3D images and NMR spectra of chick embryo and medaka by 14.1 T MR microscopy (2011)
  • Author(s): 桑原航, 上野智弘, 村島舞, 平石真寛, 山口佳美, 三浦岳, 波多野直也, 谷口善仁, 浦山慎一, 福山秀直, 酒井晃二, 杉本直三
  • Organizer: 第9回京都大学低温物質科学研究センター講演会・研究交流会
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-14
• [Presentation] High resolution 3D images and NMR spectra of chick embryo and medaka by 14.1 T MR microscopy (2011)
  • Author(s): T. Ueno, W. Kuwabara, M. Murashima, M. Hiraishi,Y. Yamaguchi, T. Miura, N. Hatano, Y. Taniguchi, S. Urayama, H. Fukuyama, K. Sakai, and N. Sugimoto
  • Organizer: ISMRM Scientific Workshop, Ultra-High Field Systems & Applications: 7t & Beyond: Progress, Pitfalls and Potential
  • Place of Presentation: Lake Louise, Alberta, Canada
  • Year and Date: 2011-02-22
• [Presentation] High resolution 3D images and NMR spectra of chick embryo and medaka by 14.1 T MR microscopy (2011)
  • Author(s): T.Ueno, W.Kuwabara, M.Murashima, M.Hiraishi, Y.Yamaguchi, T.Miura, N.Hatano, Y.Taniguchi, S.Urayama, H.Fukuyama, K.Sakai, N.Sugimoto
  • Organizer: ISMRM Scientific Workshop, Ultra-High Field Systems & Applications : 7t & Beyond : Progress Pitfalls and Potential
  • Place of Presentation: Lake Louise, Alberta, Canada
  • Year and Date: 2011-02-22
• [Presentation] 固定された脊椎動物モデルのNMRスペクトルとMRI画像の検討 (2011)
  • Author(s): 桑原航,上野智弘,村島舞,平石真寛,山口佳美,三浦岳,波多野直也,谷口善仁,浦山慎一,福山秀直,酒井晃二,杉本直三
  • Organizer: 医用画像研究会(メディカルイメージング連合フォーラム)
  • Place of Presentation: 沖縄県那覇市
  • Year and Date: 2011-01-20
• [Presentation] 固定された脊椎動物モデルのNMRスペクトルとMRI画像の検討 (2011)
  • Author(s): 桑原航, 上野智弘, 村島舞, 平石真寛, 山口佳美, 三浦岳, 波多野直也, 谷口善仁, 浦山慎一,福山秀直, 酒井晃二, 杉本直三
  • Organizer: 医用画像研究会(メディカルイメージング連合フォーラム)
  • Place of Presentation: 那霸市
  • Year and Date: 2011-01-20
• [Presentation] Development of MR Microscope and three dimensional visualization of the medaka (2010)
  • Author(s): T. Ueno, S. Kanayama, W. Kuwabara, S. Urayama, N. Hatano, Y. Taniguchi, N. Sugimoto, and H. Fukuyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2010-09-20
• [Presentation] Development of MR Microscope and three dimensional visualization of the medaka (2010)
  • Author(s): T.Ueno, S.Kanayama, W.Kuwabara, S.Urayama, N.Hatano, Y.Taniguchi, N.Sugimoto, H.Fukuyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-20
• [Presentation] MR顕微鏡の開発とメダカの3次元的可視化 (2010)
  • Author(s): T. Ueno, S. Kanayama, W. Kuwabara, S. Urayama, N. Hatano, Y. Yamaguchi, T. Miura, Y. Taniguchi, N. Sugimoto, and H. Fukuyama
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: 埼玉県さいたま市
  • Year and Date: 2010-09-18
• [Presentation] MR顕微鏡の開発とメダカの3次元的可視化 (2010)
  • Author(s): T.Ueno, S.Kanayama, W.Kuwabara, S.Urayama, N.Hatano, Y.Yamaguchi, T.Miura, Y.Taniguchi, N.Sugimoto, H.Fukuyama
  • Organizer: 第16回小型魚類研究会
  • Place of Presentation: さいたま市
  • Year and Date: 2010-09-18
• [Presentation] MRI顕微鏡によるメダカガン細胞のin situ3 次元可視化に向けた固定法の検討 (2010)
  • Author(s): 桑原航,上野智弘,浦山慎一,谷口善仁,山口佳美,三浦岳,福山秀直,杉本直三
  • Organizer: 生体医工学シンポジウム2010
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2010-09-10
• [Presentation] MRI顕微鏡によるメダカガン細胞のin situ3次元可視化に向けた固定法の検討 (2010)
  • Author(s): 桑原航, 上野智弘, 浦山慎一, 谷口善仁, 山口佳美, 三浦岳, 福山秀直, 杉本直三
  • Organizer: 生体医工学シンポジウム2010
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-10
• [Presentation] 3D Visualization of the Medaka by MR Microscope (2010)
  • Author(s): T. Ueno, S. Kanayama, W. Kuwabara, S. Urayama, N. Hatano, Y. Taniguchi, N. Sugimoto, and H. Fukuyama
  • Organizer: The 3rd Biomedical Engineering Internation Conference
  • Place of Presentation: 京都府京都市
  • Year and Date: 2010-08-27
• [Presentation] 3D Visualization of the Medaka by MR Microscope (2010)
  • Author(s): T.Ueno, S.Kanayama, W.Kuwabara, S.Urayama, N.Hatano, Y.Taniguchi, N.Sugimoto, H.Fukuyama
  • Organizer: The 3^<rd> Biomedical Engineering International Conference
  • Place of Presentation: 京都市
  • Year and Date: 2010-08-27
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/organization-staff/research/human_health/mt0601/