Visualization of gas molecules in vivo using infrared Raman Spectroscopy and application to tumor detection

• Project/Area Number: 22700476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Keio University
• Principal Investigator: TSUKADA Kosuke 慶應義塾大学, 理工学部, 専任講師 (00351883)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: がん / ラマン散乱 / 低酸素 / 生体医工学 / ラマン分光 / ガス分子 / 腫瘍 / 一酸化窒素 / 一酸化炭素 / 早期発見

Research Abstract

We developed an optical system of time-resolved Raman spectroscopy to detect and image gaseous molecules which increase in tumor or inflammatory conditions. The system has a high sensitivity for detecting Raman scattering by reducing the fluorescence back

Research Products

• [Journal Article] Visualization and analysis of blood flow and oxygen consumption in hepatic microcirculation: application to an acute hepatitis model (2012)
  • Author(s): Tsukada K. and Suematsu M
  • Journal Title: Journal of Visualized Experiments Volume: 66 Issue: 66
  • DOI: 10.3791/3996
  • Peer Reviewed
• [Journal Article] Co-culture microdevice with oxygen gradient for tumor microenvironment model and metastasis imaging (2012)
  • Author(s): Shiwa T., Uchida H. and Tsukada K
  • Journal Title: Am. J. Biomed. Eng Volume: 2(4) Pages: 175-180
  • Peer Reviewed
• [Journal Article] 腫瘍の低酸素イメージングと酸素化過程における時空間解析 (2012)
  • Author(s): 山田 遼,堀之内宏久,塚田孝祐
  • Journal Title: 電気学会論文誌 C(電子・情報・システム部門誌) Volume: 132(10) Pages: 1602-7
  • NAID: 10031120104
  • Peer Reviewed
• [Journal Article] Co-culture microdevice with oxygen gradient for tumor microenvironment model and metastasis imaging (2012)
  • Author(s): Takahiro Shiwa
  • Journal Title: American Journal of Biomedical Engineering Volume: 2 Issue: 4 Pages: 175-180
  • DOI: 10.5923/j.ajbe.20120204.04
  • Peer Reviewed
• [Journal Article] Hypoxia Imaging of Tumor and Spatiotemporal Analysis during Oxygen Inhalation (2012)
  • Author(s): 山田 遼
  • Journal Title: IEEJ Transactions on Electronics, Information and Systems Volume: 132 Issue: 10 Pages: 1602-1607
  • DOI: 10.1541/ieejeiss.132.1602
  • NAID: 10031120104
  • ISSN: 0385-4221, 1348-8155
  • Peer Reviewed
• [Journal Article] Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice (2011)
  • Author(s): Yamada R., Horinouchi H., Tsukada K
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. Volume: 2011 Pages: 5965-5968
  • Peer Reviewed
• [Journal Article] Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice (2011)
  • Author(s): Yamada R., Horinouchi H., Tsukada K.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc.2011 Volume: 2011 Pages: 5965-5968
  • DOI: 10.1109/iembs.2011.6091474
  • Peer Reviewed
• [Presentation] Fluorescence reduction in time-resolved Raman spectroscopy for the detection of circulating tumor cells (2012)
  • Author(s): Osaki H., Tamano Y. and Tsukada K
  • Organizer: 2012 Annual Meeting of BMES
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-10-27
• [Presentation] Development of a phosphore scence-based oxygen sensor using organic electroluminescence (2012)
  • Author(s): Yoshida R., Yanagisawa Y. and Tsukada K.
  • Organizer: 2012 Annual Meeting of BMES
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-10-27
• [Presentation] Development of a microfluidic device forming oxygen gradient for cell culture (2012)
  • Author(s): Uchida H., Shiwa T., Sato A. and Tsukada K
  • Organizer: 2012 Annual Meeting of BMES
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-10-25
• [Presentation] ガルバノスキャナを用いた生体組織高速酸素マッピング手法の提案と評価 (2012)
  • Author(s): 山田 遼,塚田孝祐
  • Organizer: 生体医工学シンポジウム2012
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-07
• [Presentation] 生体の顕微的血流・低酸素イメージングと腫瘍生物学への応用 (2012)
  • Author(s): 塚田孝祐
  • Organizer: 2011 年度大阪電気通信大学情報施設研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-03-12
• [Presentation] 生体の顕微的血流・低酸素イメージングと腫瘍生物学への応用 (2012)
  • Author(s): 塚田孝祐
  • Organizer: 2011年度大阪電気通信大学情報施設研究会
  • Place of Presentation: 大阪(招待講演)
  • Year and Date: 2012-03-12
• [Presentation] Development of a phosphorescence-based oxygen sensor using organic electroluminescence (2012)
  • Author(s): Rikuto Yoshida
  • Organizer: 2012 Annual Meeting of BMES
  • Place of Presentation: Atlanta, USA
• [Presentation] 生体分光計測が解き明かすがんの低酸素・糖代謝メカニズム (2011)
  • Author(s): 塚田孝祐
  • Organizer: 日本学術会議 第二回先端フォトニクスシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] 生体分光計測が解き明かすがんの低酸素・糖代謝メカニズム (2011)
  • Author(s): 塚田孝祐
  • Organizer: 日本学術会議第二回先端フォトニクスシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-09-07
• [Presentation] Spatiotemporal measurement of tumor oxygenation reveals repeat hypoxic phenomenon in mice (2011)
  • Author(s): Yamada R., Horinouchi H., Tsukada K
  • Organizer: 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-09-02
• [Presentation] Nitric oxide gradients mediate vessel maturation in solid tumors (2010)
  • Author(s): Kashiwagi S., Tsukada K., Xu L, Miyazaki J., Kozin S.V., Tyrrell J.A., Sessa W.C., L.E.Gerweck L.E., Jain R.K., Fukumura D.
  • Organizer: 9th World Congress for Microcirculation
  • Place of Presentation: Paris
  • Year and Date: 2010-09-27
• [Presentation] 顕微的酸素代謝の定量と腫瘍モデルへの応用 (2010)
  • Author(s): 塚田孝祐
  • Organizer: 第3回細胞機能可視化研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] 顕微的酸素代謝の定量と腫瘍モデルへの応用 (2010)
  • Author(s): 塚田孝祐
  • Organizer: 第3回細胞機能可視化研究会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-21
• [Presentation] 組織血流と低酸素の光計測技術:実用化と医療展開に向けて (2010)
  • Author(s): 塚田孝祐
  • Organizer: 次世代医療システム産業化フォーラム 2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-09
• [Presentation] 組織血流と低酸素の光計測技術:実用化と医療展開に向けて (2010)
  • Author(s): 塚田孝祐
  • Organizer: 次世代医療システム産業化フォーラム2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-09