Application of Fluorescent Metallo-Glycolipid for Targeting CancerCells

• Project/Area Number: 22700483
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo University of Technology
• Principal Investigator: OKADA Tomoko 東京工科大学, 片柳研究所, 研究員 (60409795)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 糖脂質 / 蛍光金属錯体 / 膜融合 / 癌細胞 / 糖鎖 / ルテニウム / 癌

Research Abstract

A novel metallo-glycolipid was designed and synthesized for giving a fluorescent carbohydrate probe that was potentially incorporated into a biological membrane. The metallo-glycolipid was succeeded in giving a fluorescent liposome with carbohydrates placed on a biomimetic liposomal membrane. The liposome was used for evaluateing recognition ability of carbohydrate to lectin. Specific binding events between lectin and carbohydrates on the liposomal surface was proved by the affinity evaluation with a fluorescent polarization analysis. Moreover, uptake of the metallo-glycocluster by HeLa cell was observed by fluorescent microscopy. These results suggest application of the metallo-glycolipid to a targeting agent for a cancer cell.

Research Products

• [Journal Article] Intricate Recognition of Glycolipid-like Compounds by HIV-1 Envelope Proteins Evaluated with Surface Plasmon Resonance Imaging (2012)
  • Author(s): T. Okada, A. Kimura, H. Miura, T.Nishiyama, M. Mori, J. Suzuki, M. Ogiso, K. Matsuoka, T. Sato, K. Hatanaka, N. Minoura.
  • Journal Title: J. Carbohydr. Chem. Volume: 31(7) Issue: 7 Pages: 584-597
  • DOI: 10.1080/07328303.2012.682190
  • Peer Reviewed
• [Journal Article] Application of Functionalized Metalloglycoclusters (2011)
  • Author(s): T. Okada.
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 23 Issue: 134 Pages: 284-291
  • DOI: 10.4052/tigg.23.284
  • NAID: 10030389401
  • ISSN: 0915-7352, 1883-2113
  • Peer Reviewed
• [Journal Article] Fluorescence Emission and Polarization Analyses for Evaluating Binding of Ruthenium Metalloglycoclusters to Lectins and Tetanus Toxin C-Fragment (2011)
  • Author(s): T. Okada, N. Minoura
  • Journal Title: J. Biomed. Opt Volume: 16 (3) Issue: 3 Pages: 37001-37001
  • DOI: 10.1117/1.3558727
  • Peer Reviewed
• [Journal Article] Application of Functionalized Metalloglycoclusters (2011)
  • Author(s): Tomoko Okada
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 23 Pages: 284-291
  • NAID: 10030389401
  • Peer Reviewed
• [Journal Article] Fluorescence Emission and Polarization Analyses for Evaluating Binding of Ruthenium Metalloglycoclusters to Lectins and Tetanus Toxin C-Fragment (2011)
  • Author(s): Tomoko Okada, Norihiko Minoura
  • Journal Title: Journal of Biomedical Optics Volume: 16 Pages: 37001-37001
  • Peer Reviewed
• [Journal Article] Fluorescence Emission and Polarization Analyses for Evaluating Binding of Ruthenium Metalloglycoclusters to Lectins and Tetanus Toxin C-Fragment (2011)
  • Author(s): Tomoko Okada, Norihiko Minoura
  • Journal Title: Virtual Journal of Biological Physics Research Volume: 21
• [Presentation] リンカー鎖長を制御したルテニウム錯体型糖鎖プローブ分子の合成およびその分子認識能評価 (2013)
  • Author(s): 今泉 竜一・岡田 朋子・箕浦 憲彦
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学（滋賀県）
• [Presentation] 糖ペプチドによる糖鎖集積化の制御とレクチン親和性 (2013)
  • Author(s): 磯部 知香・岡田 朋子・箕浦 憲彦
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学（滋賀県）
• [Presentation] 脂質を持つ新規なルテニウム錯体型糖鎖プローブ分子を利用した分子認識能評価 (2012)
  • Author(s): 田野倉大智、今泉竜一、岡田朋子、箕浦憲彦
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶応大学(神奈川県)
  • Year and Date: 2012-03-25
• [Presentation] リンカー鎖長を制御したルテニウム錯体型糖鎖プローブ分子の合成 (2012)
  • Author(s): 今泉竜一・岡田朋子・箕浦憲彦
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶応大学(神奈川県)
  • Year and Date: 2012-03-25
• [Presentation] 糖ペプチドによる糖鎖集積化とレクチン認識能評価 (2012)
  • Author(s): 磯部 知香・岡田 朋子・箕浦 憲彦
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島市民文化ホール（鹿児島県）
• [Presentation] 糖鎖とレクチン間の相互作用を評価するためのルテニウム錯体型糖脂質の創製 (2011)
  • Author(s): 田野倉大智、今泉竜一、岡田朋子、箕浦憲彦
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2011-09-12
• [Presentation] 脂質を持つルテニウム錯体型糖鎖プローブの合成 (2011)
  • Author(s): 田野倉大智、今泉竜一、岡田朋子、箕浦憲彦
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-28
• [Presentation] Application of ruthenium metalloglycocluster for affinity evaluation between carbohydrates and lectins by fluorescence emission and polarization (2010)
  • Author(s): T. Okada, T. Makino, N. Minoura
  • Organizer: Pacifichem 2010
  • Place of Presentation: Kamehameha Halls (Honolulu, USA)
  • Year and Date: 2010-12-19
• [Presentation] Application of Ruthenium Metalloglycocluster for affinity evaluation between carbohydrate and lectins by fluorescence emission and polarization (2010)
  • Author(s): Tomoko Okada, Taro Makino, Norihiko Minoura
  • Organizer: Pacifichem 2010
  • Place of Presentation: Kamehameha Halls(Honolulu)
  • Year and Date: 2010-12-19