• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of functional polymer-siRNA conjugates for siRNA delivery

Research Project

Project/Area Number 22700489
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

MIYATA Kanjiro  東京大学, 大学院・医学系研究科, 助教 (50436523)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordssiRNA デリバリー / バイオコンジュゲート材料 / siRNA / ドラッグデリバリー / 機能性高分子 / ポリイオンコンプレックス
Research Abstract

In this study, a smart siRNA-polymer conjugate was developed withan endosome-disrupting polymer via an acidic pH-responsive linkage. Ultimately, theconjugate provided siRNA with enhanced blood circulation property, and further achievedefficient gene silencing in cultured cells, probably due to facilitated endosomal escape ofsiRNA.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (55 results)

All 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (30 results) (of which Invited: 3 results) Book (1 results) Remarks (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Acidic pH-Responsive siRNA Conjugate for Reversible Carrier Stability and Accelerated Endosoraal Escape with Reduced IFNα -Associated Immune Response2013

    • Author(s)
      Hiroyasu Takemoto, Kanjiro Miyata, Shota Hattori, Takehiko Ishii, Toraoya Suma, Satoshi Uchida, Nobuhiro Nishiyama and Kazunori Kataoka
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 52 Issue: 24 Pages: 6218-6221

    • DOI

      10.1002/anie.201300178

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Silica nanogelling of environment-responsive PEGylated polyplexes for enhanced stability and intracellular delivery of siRNA2013

    • Author(s)
      N. Gouda, K. Miyata
    • Journal Title

      Biomaterials

      Volume: 34 Issue: 2 Pages: 562-570

    • DOI

      10.1016/j.biomaterials.2012.09.077

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 微小な化学構造の違いに基づき低毒性/高効率核酸送達を実現する高分子ナノ材料設計2013

    • Author(s)
      宮田完二郎
    • Journal Title

      バイオマテリアル-生体材料-

      Volume: 31 Pages: 26-34

    • Related Report
      2012 Annual Research Report
  • [Journal Article] A phenylboronate-functionalized polyion complex micelle for ATP-triggered release of siRNA2012

    • Author(s)
      M. Naito, K. Miyata
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 51 Issue: 43 Pages: 10751-10755

    • DOI

      10.1002/anie.201203360

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Smart multilayered assembly for biocompatible siRNA delivery featuring dissolvable silica, endosome-disrupting polycation, and detachable PEG2012

    • Author(s)
      T. Suma, K. Miyata
    • Journal Title

      ACS Nano

      Volume: 6 Issue: 8 Pages: 6693-6705

    • DOI

      10.1021/nn301164a

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Accelerated Polymer-Polymer Click Conjugation by Freeze-Thaw Treatment2012

    • Author(s)
      Hiroyasu Takeraoto, Kanjiro Miyata, Shota Hattori, Shigehito Osawa, Nobuhiro Nishiyama, Kazunori Kataoka
    • Journal Title

      Bioconjugate Chemsitry

      Volume: 23 Issue: 8 Pages: 1503-1506

    • DOI

      10.1021/bc300182y

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pancreatic cancer therapy by systemic administration of VEGF siRNA contained in calcium phosphate/charge-conversional polymer hybrid nanoparticles2012

    • Author(s)
      F. Pittella, K. Miyata
    • Journal Title

      J. Control.Release

      Volume: 161 Issue: 3 Pages: 868-874

    • DOI

      10.1016/j.jconrel.2012.05.005

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeted polymeric micelles for siRNA treatment of experimental cancer by intravenous injection2012

    • Author(s)
      R. J. Christie, Y. Matsumoto, K. Miyata, T. Nomoto, S. Fukushima, K. Osada, J. Halnaut, F. Pittella, H. -J. Kim, N. Nishiyama, K. Kataoka
    • Journal Title

      ACS Nano

      Volume: 6(6) Issue: 6 Pages: 5174-5189

    • DOI

      10.1021/nn300942b

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced stability and gene silencing ability of siRNA-loaded polyion complexes formulated from polyaspartamide derivatives with a repetitive array of amino groups in the side chain2012

    • Author(s)
      T.Suma, N.Nishiyama, et al
    • Journal Title

      Biomaterials

      Volume: 33 Issue: 9 Pages: 2770-2779

    • DOI

      10.1016/j.biomaterials.2011.12.022

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rational design of smart supramolecular assemblies for gene delivery : Chemical challenges in the creation of artificial viruses2012

    • Author(s)
      K. Miyata, N. Nishiyama, K. Kataoka
    • Journal Title

      Chem. Soc. Rev

      Volume: 41(7) Issue: 7 Pages: 2562-2574

    • DOI

      10.1039/c1cs15258k

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PEG-detachable cationic polyaspartamide detivatives bearing stearoyl moieties for systemic siRNA delivery toward subcutaneous BxPC3 pancreatic tumor2011

    • Author(s)
      H.-J.Kim, N.Nishiyama, et al
    • Journal Title

      J.Drug Target.

      Volume: 20 Issue: 1 Pages: 33-42

    • DOI

      10.3109/1061186x.2011.632010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Odd-even effect of repeating aminoethylene units in the side chain of N-substituted polyaspartamides on gene transfection profiles2011

    • Author(s)
      H.Uchida, N.Nishiyama, et al
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 133 Issue: 39 Pages: 15524-15532

    • DOI

      10.1021/ja204466y

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of polymer structure on micelles formed between siRNA and cationic block copolymer Comprising thiols and amidines2011

    • Author(s)
      R. J. Christie, K. Miyata, Y. Matsumoto, T. Nomoto, D. Menasco, T.-C. Lai, M. Pennisi, K. Osada, S. Fukushima, N. Nishiyama, Y. Yamasaki, K. Kataoka
    • Journal Title

      Biomacromolecules

      Volume: 12(9) Issue: 9 Pages: 3174-3185

    • DOI

      10.1021/bm2006714

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced endosomal escape of siRNA-incorporating hybrid nanoparticles from calcium phosphate and PEG-block charge-conversional polymer for efficient gene knockdown with negligible cytotoxicity2011

    • Author(s)
      F. Pittella, M. Zhang, Y. Lee, H.-J. Kim, T. Tockary, K. Osada, T. Ishii, K. Miyata, N. Nishiyama
    • Journal Title

      Biomaterials

      Volume: 32(11) Issue: 11 Pages: 3106-3114

    • DOI

      10.1016/j.biomaterials.2010.12.057

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polymeric micelles for nano-scale drugdelivery2011

    • Author(s)
      K. Miyata, R. J. Christie, K. Kataoka
    • Journal Title

      React. Funct. Polym

      Volume: 71 Issue: 3 Pages: 227-234

    • DOI

      10.1016/j.reactfunctpolym.2010.10.009

    • Related Report
      2012 Final Research Report
  • [Journal Article] siRNA-grafted polymer for polyion complex(PIC)-based siRNA delivery : Enhancement of PIC stability and gene silencing efficiency2010

    • Author(s)
      H. Takemoto, A. Ishii, K. Miyata, M. Nakanishi, M. Oba, T. Ishii, Y. Yamasaki, N. Nishiyama, K. Kataoka
    • Journal Title

      Biomaterials

      Volume: 31(31) Issue: 31 Pages: 8097-8105

    • DOI

      10.1016/j.biomaterials.2010.07.015

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of stearoyl moieties into a biocompatible cationic polyaspartamide derivative, PAsp(DET), with endosomal escaping function for enhanced siRNA-mediated gene knockdown2010

    • Author(s)
      H.-J. Kim, A. Ishii, K. Miyata, Y. Lee, S. Wu, M. Oba, N. Nishiyama, K. Kataoka
    • Journal Title

      J. Control. Release

      Volume: 145(2) Issue: 2 Pages: 141-148

    • DOI

      10.1016/j.jconrel.2010.03.019

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] siRNA-conjugated charge-conversional polymer as a smart material for siRNA delivery2013

    • Author(s)
      H. Takemoto, K. Miyata
    • Organizer
      16th International Symposium on Recent Advances in Drug Delivery Systems
    • Place of Presentation
      シェラトンソルトレイクシティ(ユタ, 米国)
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNA-conjugated charge-conversional polymer as a smart material for siRNA delivery2013

    • Author(s)
      Hiroyasu Takemoto, Kanjiro Miyata, et al
    • Organizer
      16th International Symposium on Recent Advances in Drug Delivery Systems
    • Place of Presentation
      シェラトンソルトレイクシティ、ユタ州、アメリカ合衆国
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微小な化学構造の違いに基づいて低毒性/高効率核酸送達を実現する高分子ナノ材料設計2012

    • Author(s)
      宮田完二郎
    • Organizer
      2012年度日本バイオマテリアル学会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2012-11-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Fine-tuning of polycationic nanomaterials for enhanced nucleic acid delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      6th Annual Symposium on Nanobiotechnology Kyoto Cell-Material Integration
    • Place of Presentation
      京都大学芝蘭会館(京都府)
    • Year and Date
      2012-11-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Design of functional polymeric nanomaterials for nucleic acid delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      Oligonucleotide Delivery
    • Place of Presentation
      ヘルンシュタイン城(ヘルンシュタイン, オーストリア)
    • Year and Date
      2012-10-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Smart multilayered polymer/silica assemblies for enhanced siRNA delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      IUMRS-ICEM2012
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Self-assembled nanostructures of block copolymers as smart vehicles for gene and siRNA delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      ISACS9
    • Place of Presentation
      アモイ大学(アモイ、中国)
    • Year and Date
      2012-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Smart multilayered polymer/silica assemblies for siRNA delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      NanoBio Seattle
    • Place of Presentation
      ベルハーバーインターナショナルコンベンションセンター(ワシントン州、米国)
    • Year and Date
      2012-07-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 核酸デリバリーを指向したカチオン性ポリアミノ酸構造の精密設計2012

    • Author(s)
      宮田完二郎
    • Organizer
      第28回日本DDS学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2012-07-04
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNAデリバリー用カチオン性ポリアミノ酸設計2012

    • Author(s)
      宮田完二郎
    • Organizer
      第61回高分子年次大会
    • Place of Presentation
      パシィコ横浜(神奈川県)
    • Year and Date
      2012-05-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of cationic poly(amino acid)s for preparation of polyion complexes with nucleic acids for efficient gene and siRNA delivery2012

    • Author(s)
      K. Miyata
    • Organizer
      ACS Spring 2012 National Meeting & Exposition
    • Place of Presentation
      サンディエゴコンベンションセンター(カリフォルニア州、米国)
    • Year and Date
      2012-03-25
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNA-grafted Polymer for Efficient siRNA Delivery System2012

    • Author(s)
      H.Takemoto, K.Miyata, et al
    • Organizer
      243rd American Chemical Society National Meeting & Exposition
    • Place of Presentation
      Manchester Grand Hyatt(米国カリフォルニア州)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enhanced siRNA-polymer click conjugation after gradual freeze-thawing2012

    • Author(s)
      H. Takemoto, K. Miyata
    • Organizer
      The 9th SPSJ International Polymer Conference (IPC2012)
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Enhanced siRNA-polymer click conjugation after gradual freeze-thawing2012

    • Author(s)
      Hiroyasu Takemoto, Kanjiro Miyata, et al
    • Organizer
      The 9th SPSJ International Polymer Conference (IPC2012)
    • Place of Presentation
      神戸国際会議場、兵庫県、日本
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微小な化学構造の違いに基づいて低毒性/高効率核酸送達を実現する高分子ナノ材料設計2012

    • Author(s)
      宮田完二郎
    • Organizer
      日本バイオマテリアル学会大会シンポジウム2012
    • Place of Presentation
      仙台国際会議場、宮城県、日本
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Fine-tuning of polycationic nanomaterials for enhanced nucleic acid delivery2012

    • Author(s)
      Kanjiro Miyata, et al
    • Organizer
      6th Annual Symposium on Nanobiotechnology Kyoto Cell-Material Integration 2012
    • Place of Presentation
      京都大学芝蘭会館、京都府、日本
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Design of functional polymeric nanomaterials for nucleic acid delivery2012

    • Author(s)
      Kanjiro Miyata, et al
    • Organizer
      Oligonucleotide Delivery: Biology, Engineering and Development
    • Place of Presentation
      ヘルンシュタイン城、オーストリア
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 安全かつ高効率な核酸デリバリーに向けたカチオン性ポリアミノ酸設計2011

    • Author(s)
      宮田完二郎
    • Organizer
      第21回日本MRS学術シンポジウム
    • Place of Presentation
      横浜情報文化センター(神奈川県)
    • Year and Date
      2011-12-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Design of polymeric nanocarrier for siRNA delivery2011

    • Author(s)
      K. Miyata
    • Organizer
      France-Japan Workshop-The Nanotech Revolution from Science to Society: A Time for Passion and A Time for Reason
    • Place of Presentation
      ENS Cachan(パリ、仏国)
    • Year and Date
      2011-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Design of Polymeric Nanocarrier for siRNA Delivery2011

    • Author(s)
      K.Miyata, et al
    • Organizer
      France-Japan Workshop -The Nanotech Revolution from Science to Society-
    • Place of Presentation
      ENS Cahan(仏国パリ)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] ポリイオンコンプレックス型核酸デリバリーシステム構築に向けたカチオン性ポリアミノ酸構造の検討2011

    • Author(s)
      宮田完二郎
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Year and Date
      2011-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNAグラフト共重合体を基盤とするPIC型スマートキャリアの構築2011

    • Author(s)
      武元宏泰、宮田完二郎, 他
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Efficient siRNA Delivery Using siRNA-grafted Polymer-based Polyion Complexes2011

    • Author(s)
      H.Takemoto, K.Miyata, et al
    • Organizer
      38th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Gaylord National Hotel (米国メリーランド州)
    • Year and Date
      2011-08-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] シリカ被覆型核酸デリバリーキャリアの開発2011

    • Author(s)
      宮田完二郎
    • Organizer
      第27回日本DDS学会学術集会
    • Place of Presentation
      東大本郷キャンパス(東京都)
    • Year and Date
      2011-06-10
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNA-grafted polymer for polyion complex(PIC)-based siRNA delivery2011

    • Author(s)
      H.Takemoto, K.Miyata, et al
    • Organizer
      ICBS2011
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 有機無機ハイブリッド型核酸デリバリーキャリアの開発2010

    • Author(s)
      宮田完二郎
    • Organizer
      第19回ポリマー材料フォーラム
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2010-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] siRNA・ポリアスパラギン酸グラフト共重合体を基盤とするPIC型siRNAデリバリー2010

    • Author(s)
      武元宏泰、宮田完二郎, 他
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学高等教育機能開発総合センター(北海道札幌市)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of advanced polymer-based nanodevices for siRNA delivery2010

    • Author(s)
      K.Miyata, et al
    • Organizer
      240th ACS National Meeting
    • Place of Presentation
      ボストンコンベンションセンター(米国マサチューセッツ州)
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高分子を基盤とする遺伝子キャリアのシリカコーティングとその機能評価2010

    • Author(s)
      宮田完二郎
    • Organizer
      第26回日本DDS学会学術集会
    • Place of Presentation
      大阪国際交流センター(大阪府)
    • Year and Date
      2010-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] 低毒性かつ高効率な遺伝子導入を可能とする高分子キャリアシステムの設計.2010

    • Author(s)
      宮田完二郎
    • Organizer
      第59回高分子年次大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2010-05-27
    • Related Report
      2012 Final Research Report
  • [Book] ここまで広がるドラッグ徐放技術の最前線-古くて新しいドラッグデリバリーシステム(DDS)-2013

    • Author(s)
      武元宏泰、宮田完二郎
    • Total Pages
      376
    • Publisher
      メディカルドゥ
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bmw.t.u-tokyo.ac.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.cdbim.m.u-tokyo.ac.jp/research/04.php

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京大学 片岡研究室ホームページ

    • URL

      http://www.bmw.t.u-tokyo.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京大学 大学院医学系研究科 疾患生命工学センター 臨床医工学部門ホームページ

    • URL

      http://www.cdbim.m.u-tokyo.ac.jp/research/04.php

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://first-pg.jp/images/stories/30/09/NewsLetterVol2_j.pdf

    • Related Report
      2011 Annual Research Report
  • [Remarks] 所属研究室のURL

    • URL

      http://www.bmw.t.u-tokyo.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] アニオン性ポリマー,ニオン性ポリマーを用いたポリイオンコンプレックスおよび三元系ポリマー複合体,ならびに薬学組成物2010

    • Inventor(s)
      片岡一則,井篤史,武元宏泰,中西政崇,西山伸宏,田完二郎
    • Industrial Property Rights Holder
      東京大学
    • Filing Date
      2010-07-23
    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi