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Development of new type of temperature-responsive cell culture surfaces with stretchable properties

Research Project

Project/Area Number 22700496
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Women's Medical University

Principal Investigator

AKIYAMA Yoshikatsu  東京女子医科大学, 医学部, 講師 (20349640)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsPDMS / poly(N-isopropylacrylamide) / 電子線照射 / 超薄膜 / 細胞シート工学 / 温度応答性高分子 / 細胞シート / 再生医療
Research Abstract

A method of mechanical-assisted control of the polymer thickness and density of temperature-responsive cell culture surfaces was developed by grafting PIPAAm onto PDMS surfaces. Hydrophobicity of the PIPAAm-PDMS surfaces was readily controlled by stretching the surfaces. Further investigation of PIPAAm-PDMS surfaces demonstrated that this hydrophobicity control is because the polymer thickness and density is altered by the stretching. This result suggests that such control may attain producing cell adhesive surfaces appropriate for variety of target cells by stretching one PIPAAm-PDMS surfaces.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 Other

All Presentation (6 results) Book (1 results)

  • [Presentation] 伸縮性を有する基材を利用した温度応答性細胞培養表面の特性2011

    • Author(s)
      秋山義勝、大和雅之、岡野光夫
    • Organizer
      第33回日本バイオマテリアル学会
    • Place of Presentation
      京都
    • Year and Date
      2011-11-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 伸縮性を有する基材を利用した温度応答性細胞培養表面の特性2011

    • Author(s)
      秋山義勝、大和雅之、岡野光夫
    • Organizer
      第33回日本バイオマテリアル学会
    • Place of Presentation
      京都テレサ
    • Year and Date
      2011-11-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 伸縮性を有する温度応答性細胞培養表面の開発2011

    • Author(s)
      秋山義勝、大和雅之、岡野光夫
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山
    • Year and Date
      2011-09-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 伸縮性を有する温度応答性細胞培養表面の開発2011

    • Author(s)
      秋山義勝、大和雅之、岡野光夫
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] 伸縮性を有する基材を利用した温度応答性細胞培養表面の開発2010

    • Author(s)
      秋山義勝
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 伸縮性を有する基材を利用した温度応答性細胞培養表面の開発

    • Author(s)
      秋山義勝、大和雅之、岡野光夫
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道(札幌)
    • Related Report
      2011 Final Research Report
  • [Book] Comprehensive Biotechnology(Second Edition) volume 5, Medical Biotechnology and Healthcare Chapter 436.Biomaterials/Temperature sensitive polymer2011

    • Author(s)
      岡野光夫、秋山義勝、小林純、大和雅之
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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