Neural differentiation of human induced pluripotent stem cells by temperature-sensitive Sendai virus vectors

• Project/Area Number: 22700501
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: DNAVEC Corporation
• Principal Investigator: SAEKI Koichi ディナベック株式会社, 技術開発室, 研究員 (40360052)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 再生医学 / 細胞・組織 / 発生・分化 / バイオテクノロジー / 脳神経疾患

Research Abstract

Sendai virus(SeV) vectors can efficiently introduce foreign genes without genomic insertion of transgenes into various organs and are expected to be clinically applicable. We improved the SeV vectors by introducing temperature-sensitive mutations into the SeV backbone so that the vectors could be easily removed at nonpermissive temperatures. We obtained dopamine-producing neurons by geneX expression in induced pluripotent stem cells(iPSCs). These vectors enabled the efficient generation of differentiated cells from iPSCs.

Research Products

• [Journal Article] Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells : application to liver-specific functional and cytotoxic assays (2012)
  • Author(s): Nakamura N, Saeki K, Mitsumoto M, Matsuyama S, Nishio M, Saeki K, Hasegawa M, Miyagawa Y, Ohkita H, Kiyokawa N, Toyoda M, Akutsu H, Umezawa A, Yuo A.
  • Journal Title: Cell Reprogram Volume: 14 Issue: 2 Pages: 171-185
  • DOI: 10.1089/cell.2011.0064
  • Peer Reviewed
• [Journal Article] Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors (2011)
  • Author(s): Ban H, et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 34 Pages: 14234-14239
  • DOI: 10.1073/pnas.1103509108
  • Peer Reviewed
• [Presentation] A Feeder-free and Serum-free Production of Multi-Functional Mature Hepatocytes with Electron Microscopically Valid Morphologies : Towards an Establishment of the Global Standard for Human ES/iPS-based Drug Discovery Tools (2011)
  • Author(s): Saeki K, Nakamura N, Matsuyama S, Nishio M, Akutsu H, Umezawa A, Saeki K, Hasegawa M, Yuo A.
  • Organizer: ISSCR 9th Annual Meeting
  • Place of Presentation: Toronto, Canada
• [Presentation] Endothel-Medaited Vascular Smooth Muscle Growth Suppression is Deteriorated by Senescence-Associated Degeneration via an Induction of Gene X : Toward Establishment of Innovative Therapeutics for Angiostenosis (2011)
  • Author(s): Saeki K, Nishio M, Yuo A, Saeki K, Hasegawa M.
  • Organizer: 6th Annual Frontiers of Clinical Investigation Symposium
  • Place of Presentation: San Diego, USA
• [Presentation] A Novel System for the Evaluation of Vascular Endothel-Mediated Smooth Muscle Growth Inhibition : Towards Development of Novel Therapeutics for Arteriostenosis (2011)
  • Author(s): Saeki K, Nishio M, Nakahara M, Saeki K, Hasegawa M, Yuo A.
  • Organizer: BIT's 4th Annual World Congress of Regenerative Medicine & Stem Cell 2011
  • Place of Presentation: Bejing, China
• [Presentation] EMPERATURE SENSITIVE SENDAI VIRUS VECTORS ENABLE TO GENERATE COMPLETELY TRANSGENE-FREE HUMAN IPS CELLS EFFICIENTLY. (2010)
  • Author(s): Noemi Fusaki, Hiroshi Ban, Toshiaki Tabata, Koichi Saeki, Mamoru Hasegawa
  • Organizer: ISSCR 8th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2010-06-18
• [Presentation] Simple and Efficient Preparation of Immaculate Human iPS Cells with Transgene-Free and No Chromosomal Damages Using an Extranuclear Manipulation System of Genetic Information, PlasmExTM? (2010)
  • Author(s): Noemi Fusaki, Hiroshi Ban, Koichi Saeki, Toshiaki Tabata, Makoto Inoue, Mamoru Hasegawa.
  • Organizer: 13th ASGCT annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2010-05-22
• [Presentation] Erasable Non-Integrating RNA Replicon Vectors for Generation of Immaculate iPS Cells Free from Transgenes and Sequence-Interference (2010)
  • Author(s): Hiroshi Ban, Noemi Fusaki, Toshiaki Tabata, Koichi Saeki, Akihiro Iida, Makoto Inoue, Mamoru Hasegawa.
  • Organizer: 13th ASGCT annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2010-05-21