Basic study for the development of a new organ preservation solution using insect derived dormancy peptide

• Project/Area Number: 22700502
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical systems
• Research Institution: Iwate University
• Principal Investigator: KATAYAMA Masaaki 岩手大学, 農学部, 准教授 (70436054)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 昆虫 / 休眠 / 臓器移植 / 臓器保存 / ペプチド / C16-Yamamarin / Feline kidney distal tubular cells / WST-8アッセイ / LDH活性 / リン酸緩衝ショ糖液 / ポリエチレングリコール / 乳酸脱水素酵素(LDH)活性 / アデノシン三リン酸(ATP)活性

Research Abstract

Cryopreservation and rewarming of feline kidney cells with an existing intracellular and extracellular organ preservation solution added C16-Yamamarin, an insect derived dormancy peptide, was indicated dormancy effects of C16-Yamamarin to the feline kidney cells which were reversible. Although the organ preservation effects of C16-Yamamarin were not provided in this study, it may suggest the possibility of the application of C16-Yamamarin as an organ preservative.