Roles of autophagy in slow-twitch skeletal muscle
| Project/Area Number |
22700656
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Sports science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | スポーツ性科学 / 廃用性筋萎縮 / オートファジー / Parkin / ミトコンドリア / 骨格筋 / 筋萎縮 / ノックアウトマウス / マイトファジー / ヒラメ筋 / Atg7ノックアウトマウス |
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| Research Abstract |
To evaluate the role of autophagy in skeletal muscle atrophy, we subjected skeletal muscle specific Atg7 deficient(Atg7 KO) mice to denervation, a model of skeletal muscle atrophy. Soleus muscle, a slow-twitch skeletal muscle, from Atg7 KO mice maintained muscle mass, while Soleus muscle from control mice, and first-twitch muscles from both wild type and Atg7 KO mice were atrophied. In the denervated Soleus muscles from Atg7 KO mice, the reduction of mitochondrial activity, the accumulation of oxidative stress and the accumulation of Parkin in mitochondria were observed. Parkin participates for mitochondrial quality control(Parkin mediated mitophagy). To confirm whether Parkin mediated mitophagy is involved in the restoration of denervation atrophy in Atg7 KO Soleus muscle, we subjected Parkin deficient(Parkin KO) mice to denervation. Like Atg7 KO Soleus, denervated Soleus muscles from Parkin KO mice maintained muscle mass. These results indicate that Parkin mediated mitophagy is involved in denervation atrophy in slow-twitch skeletal muscles.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels2011
Author(s)
Ezaki, J., Matsumoto, N., Takeda-Ezaki, M., Komatsu, M., Takahashi, K., Hiraoka, Y., Taka, H., Fujimura, T., Takehana, K., Yoshida, M., Iwata, J., Tanida, I., Furuya, N., Zheng, D. M., Tada, N., Tanaka, K., Kominami, E., Ueno, T.
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Journal Title
Autophagy
Volume: 7(7)
Pages: 727-36
Related Report
Peer Reviewed
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[Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels2011
Author(s)
Ezaki, J., Matsumoto, N., Takeda-Ezaki, M., Komatsu, M., Takahashi, K., Hiraoka, Y., Taka, H., Fujimura, T., Takehana, K., Yoshida, M., Iwata, J., Tanida, I., Furuya, N., Zheng, D-m., Tada, N., Tanaka, K., Kominami, E., Ueno, T.
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Journal Title
Autophagy
Volume: 7
Issue: 7
Pages: 727-736
DOI
Related Report
Peer Reviewed
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