Research on the role of microRNA in skeletal muscle atrophy

• Project/Area Number: 22700669
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所) (2011); Gifu International Institute of Biotechnology (2010)
• Principal Investigator: FUJITA Yasunori 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (30515888)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 筋萎縮 / microRNA / 筋委縮 / 老化 / マイクロアレイ

Research Abstract

In an attempt toreveal the molecular mechanism of skeletal muscle atrophy, we identified microRNAs(miRNAs) that were related to atrophy by microarray analysisand investigatedtheirfunctionin mousemyoblast C2C12 cells. Resultssuggested that mmu-miR-148apromotesmuscleatrophy of differentiated C2C12 cells. It is necessary to verify its usefulness as a molecular targetfor the treatment andprevention ofskeletal muscle atrophy by more detailed research.

Research Products

• [Journal Article] MicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation (2012)
  • Author(s): Yasunori Fujita, Nanako Hamada, Toshio Kojima, Aya Kitamoto, Yukihiro Akao, Yoshinori Nozawa, Masafumi Ito
  • Journal Title: Neurochem Int Volume: 60 Issue: 8 Pages: 743-750
  • DOI: 10.1016/j.neuint.2012.03.010
  • Peer Reviewed
• [Journal Article] MicroRNA expression profiling of NGF-treated PC12 cells revealed a critical role for miR-221 in neuronal differentiation (2012)
  • Author(s): Yasunori Fujita
  • Journal Title: Neurochem Int Volume: (in press)
  • Peer Reviewed
• [Journal Article] MiR-148a attenuates paclitaxel-resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression (2010)
  • Author(s): Yasunori Fujita, Keitaro Kojima, Riyako Ohhashi, Nanako Hamada, Yoshinori Nozawa, Aya Kitamoto, Akira Sato, Shinji Kondo, Toshio Kojima, Takashi Deguchi, Masafumi Ito
  • Journal Title: J Biol Chem Volume: 285 Issue: 25 Pages: 19076-84
  • DOI: 10.1074/jbc.m109.079525
  • Peer Reviewed
• [Presentation] MiR-130a attenuates paclitaxel resistance of drug-resistant prostate cancer PC3 cells through post-transcriptional regulation of MSK1 expression (2011)
  • Author(s): Yasunori Fujita, Toshio Kojima, Keita Nakane, Taku Kato, Keitaro Kojima, Nanako Hamada, Riyako Ohhashi, Takashi DeguchiYoshinori Nozawa, Masafumi Ito
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] MicroRNA expression profiling of NGF-treated PC12 cells revealed critical roles of miR-221 in neuronal differentiation (2011)
  • Author(s): Nanako Hamada, Yasunori Fujita, Toshio Kojima, Yukihiro Akao, Yoshinori Nozawa, Masafumi Ito
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13