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Analysis of GADD34 function in aging, nutrient control and wound healing

Research Project

Project/Area Number 22700691
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied health science
Research InstitutionNagoya University

Principal Investigator

ITO Sachiko  名古屋大学, 医学系研究科, 助教 (70447845)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsGADD34 / mTORシグナル / 絶食ストレス / 創傷治癒 / 栄養欠乏ストレス / TORシグナル伝達系 / 老化 / 栄養制御
Research Abstract

In this study, we analyzed the function of Growth arrest and DNA damage protein 34 (GADD34) in aging, nutrient deficient-stress and wound healing by using GADD34 Knock out(KO)mice. Starvation induced the expression of GADD34 in the liver. GADD34 bound to and dephosphorylated pTSC2. The dephosphorylation of pTSC2 leads to the suppression of mTOR, which isa potent inhibitor of autophagy. The starvation-induced GADD34 suppressed mTOR and induced autophagy. In wound healing, the rate of wound closure was faster in GADD34 KO MEFs (mouse embryonic fibroblasts) than in wild type(WT) MEFs. WT mice expressed higher amounts of myosin IIA in migrating macrophages and myofibroblasts than in GADD34 KO mice. These results indicate that GADD34 negatively regulates cell migration in wound healing via expression of Myosin IIA.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (14 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (9 results)

  • [Journal Article] Autophagic activity in thymus and liver during aging.2012

    • Author(s)
      Uddin MN
    • Journal Title

      Age(Dordr)

      Volume: 34(1) Issue: 1 Pages: 75-85

    • DOI

      10.1007/s11357-011-9221-9

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gadd34 induces autophagy through the suppression of the mTOR pathway during starvation2011

    • Author(s)
      Uddin MN, Ito S, Nishio N, Suganya T, Isobe K
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 407(4) Issue: 4 Pages: 692-8

    • DOI

      10.1016/j.bbrc.2011.03.077

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagic activity in thymus and liver during aging.2011

    • Author(s)
      Mohammad Nizam Uddin
    • Journal Title

      Age (Dordr)

      Volume: (印刷中 未定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GADD34 suppresses wound healing by upregulating expression of myosin IIA2010

    • Author(s)
      Tanaka C, Ito S, Nishio N, Kodera Y, Sakurai H, Suzuki H, Nakao A, Isobe K
    • Journal Title

      Transgenic Res

      Volume: 19(4) Issue: 4 Pages: 637-45

    • DOI

      10.1007/s11248-009-9347-z

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] GADD34 suppresses wound healing by upregulating expression of myosin IIA2010

    • Author(s)
      田中千恵
    • Journal Title

      Transgenic research

      Volume: 19 Pages: 637-645

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] GADD34 regulates the LPS-induced cytokine production via TSC-mTOR signaling pathway2011

    • Author(s)
      伊藤佐知子、西尾尚美、鈴木治彦、磯部健一
    • Organizer
      第40回日本免疫学会総会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-27
    • Related Report
      2011 Final Research Report
  • [Presentation] The loss of GAD34 gene induced the abnormal expansion of neutrophil2011

    • Author(s)
      西尾尚美、伊藤佐知子、鈴木治彦、磯部健一
    • Organizer
      第40回日本免疫学会総会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-27
    • Related Report
      2011 Final Research Report
  • [Presentation] GADD34遺伝子欠損マウスは老化に伴いミエロ系細胞が異常増殖する2011

    • Author(s)
      西尾尚美、伊藤佐知子、磯部健一
    • Organizer
      第34回日本基礎老化学会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-15
    • Related Report
      2011 Final Research Report
  • [Presentation] GADD34 regulates the LPS-induced cytokine production via TSC-mTOR signaling pathway.2011

    • Author(s)
      伊藤佐知子
    • Organizer
      第40回 日本免疫学会総会
    • Place of Presentation
      千葉、幕張メッセ
    • Related Report
      2011 Annual Research Report
  • [Presentation] The loss of GAD34 gene induced the abnormal expansion of neutrophil.2011

    • Author(s)
      西尾尚美
    • Organizer
      第40回 日本免疫学会総会
    • Place of Presentation
      千葉、幕張メッセ
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス骨髄由来樹状細胞における GADD34 の役割と老化との関連2010

    • Author(s)
      伊藤佐知子、西尾尚美、磯部健一
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-06-17
    • Related Report
      2011 Final Research Report
  • [Presentation] GADD34の生体内機能と老化への関与2010

    • Author(s)
      西尾尚美、伊藤佐知子、磯部健一
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-06-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 磯部健一 胸腺の老化と胸腺上皮細胞の変化2010

    • Author(s)
      Mohammad NizamUddin、伊藤佐知子、西尾尚美、Zhao Cheng 、Zhang Rong
    • Organizer
      第33回日本基礎老化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-06-17
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス骨髄由来樹状細胞におけるGADD34の役割と老化の関連2010

    • Author(s)
      伊藤佐知子
    • Organizer
      第33回 日本基礎老化学会
    • Place of Presentation
      野依記念学術交流会館(名古屋大学、名古屋市)
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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