Proteomic analysis of nitrated proteins that change with aging and physical exercise in the brain
Project/Area Number |
22700701
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied health science
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Research Institution | Juntendo University |
Principal Investigator |
UDA Munehiro 順天堂大学, 医学研究科, 博士研究員 (80549262)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 加齢 / 脳 / ニトロ化タンパク質 / ニトロトリプトプァン / 身体運動 / 酸化修飾 / 6-ニトロトリプトファン / プロテオミクス / 海馬 / 3-ニトロチロシン / 学習・記憶機能 |
Research Abstract |
We identified twelve 6-nitrotyptophan-containing proteins including several cytoskeletal proteins and glycolytic enzymes in the adult rat hippocampus and determined the position of nitrated tryptophan residue with significant ion score levels(p<0.05) in several proteins by proteomic analysis. In addition, the amount of 6-nitrotyptophan-containing protein tended to increase with age. On the other hand, physical exercise on the 12 months old rats did not induce much difference in the positive spots of anti-6-nitrotryptophan antibody.
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Report
(3 results)
Research Products
(11 results)