Proteomic analysis of nitrated proteins that change with aging and physical exercise in the brain

• Project/Area Number: 22700701
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: Juntendo University
• Principal Investigator: UDA Munehiro 順天堂大学, 医学研究科, 博士研究員 (80549262)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 加齢 / 脳 / ニトロ化タンパク質 / ニトロトリプトプァン / 身体運動 / 酸化修飾 / 6-ニトロトリプトファン / プロテオミクス / 海馬 / 3-ニトロチロシン / 学習・記憶機能

Research Abstract

We identified twelve 6-nitrotyptophan-containing proteins including several cytoskeletal proteins and glycolytic enzymes in the adult rat hippocampus and determined the position of nitrated tryptophan residue with significant ion score levels(p<0.05) in several proteins by proteomic analysis. In addition, the amount of 6-nitrotyptophan-containing protein tended to increase with age. On the other hand, physical exercise on the 12 months old rats did not induce much difference in the positive spots of anti-6-nitrotryptophan antibody.

Research Products

• [Journal Article] Nitration of tryptophan in ribosomal proteins is a novel post-translational modification of differentiated and naive PC12 cells (2011)
  • Author(s): Kawasaki H, Shigenaga A, Uda M, Baba T, Ogawa H, Takamori K, Yamakura F
  • Journal Title: Nitric Oxide Volume: 25(2) Pages: 176-182
  • Peer Reviewed
• [Presentation] ラットの海馬と小脳における6-ニトロトリプトファン含有タンパク質のプロテオーム解析 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第33回日本トリプトファン研究会(JSTRY)学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-12-04
• [Presentation] ラットの海馬と小脳における6-ニトロトリプトファン含有タンパク質のプロテオーム解析 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第33回日本トリプトファン研究会(JSTRY)学術集会
  • Place of Presentation: 東邦大学、千葉県
  • Year and Date: 2011-12-04
• [Presentation] ラットの海馬における6-ニトロトリプトファン含有タンパク質のプロテオーム解析 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] ラットの海馬における6-ニトロトリプトファン含有タンパク質のプロテオーム解析 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館、京都府
  • Year and Date: 2011-09-22
• [Presentation] ラットの海馬におけるニトロ化タンパク質に対する走運動の影響 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 山口
  • Year and Date: 2011-09-16
• [Presentation] ラットの海馬におけるニトロ化タンパク質に対する走運動の影響 (2011)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第66回日本体力医学会大会
  • Place of Presentation: 海峡メッセ下関・下関生涯学習プラザ、山口県
  • Year and Date: 2011-09-16
• [Presentation] Differences in the amounts of nitrated proteins and superoxide dismutase in hippocampus of adult and middle age rats (2011)
  • Author(s): Munehiro Uda, Hiroaki Kawasaki, Fumiyuki Yamakura
  • Organizer: The Inaugural International Academy of Sportology
  • Place of Presentation: 東京
  • Year and Date: 2011-05-03
• [Presentation] Changes in nitrated protein content and expressions of superoxide dismtase and nitric oxide syntase in the hippocampus of adult and middle aged rat (2011)
  • Author(s): Munehiro Uda, Hiroaki Kawasaki, Fumiyuki Yamakura
  • Organizer: Experimental Biology 2011
  • Place of Presentation: ワシントンD. C., U. S. A
  • Year and Date: 2011-04-13
• [Presentation] Changes in tyrosine-nitrated proteins in the hippocampus of adult- and middle-aged rat (2011)
  • Author(s): Munehiro Uda, Hiroaki Kawasaki, Ayako Shigenaga, Fumiyuki Yamakura
  • Organizer: Experimental Biology 2011
  • Place of Presentation: Walter E.Washington Convention Center, U.S.A.
  • Year and Date: 2011-04-13
• [Presentation] Differences in the amounts of nitrated proteins and superoxide dismutase in hippocampus of adult and middle age rats (2011)
  • Author(s): Munehiro Uda, et al.
  • Organizer: International Academy of Sportology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-03-05