Isolation of peptides with antioxidant activity from biodiesel byproducts by autofocusing, which could be applied to the food system

• Project/Area Number: 22700736
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kyoto Prefectural University
• Principal Investigator: PARK Eunyoung 京都府立大学, 食保健学科, 助手 (50405239)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 食品と貯蔵 / 抗酸化 / ペプチド / 大量分画法 / 大量分画

Research Abstract

Gluten and soy protein hydrolysates were fractionated on the basis of the amphoteric nature of sample peptides by autofocusing. Antioxidant activities of the autofocusing fractions were evaluated by DPPH and OH radical scavenging assays, ORAC assay, and so on. Also, the cooked pork patties added with peptides were stored at 4℃, 20℃in the dark. After incubation, lipid oxidation of the cooked pork patties was tested using TBA method. All autofocusing fractions of gluten hydrolysate and acidic, weak acidic fractions of soy protein hydrolysate suppressed oxidation of lipid in the patties.

Research Products

• [Journal Article] Effects of the addition of gluten hydrolysates and their fractions with Different pI on Lipid Oxidation in Pork Meat Patties
  • Author(s): Park, E. Y., Imazu, H., Matsumura, Y., Nakamura, Y., Sato, K.
  • Journal Title: J. Agric. Food Chem Volume: (投稿中)
  • Peer Reviewed
• [Presentation] 食品由来ペプチドのin vitro系および食品モデル系での抗酸化活性 (2012)
  • Author(s): 朴恩榮、山田聡子、中村考志、佐藤健司
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 食品由来ペプチドのin vitro系および食品モデル系での抗酸化活性 (2012)
  • Author(s): 朴恩榮
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学(京都府)(招待講演)
  • Year and Date: 2012-03-23
• [Book] New York, Proteins and peptides as antioxidants, In : Bioactive Food Proteins and Peptides : Applications in Human Health (2012)
  • Author(s): Kim, S. K., Wijesekara, I., Park, E. Y., Matsumura, Y., Nakamura, Y., Sato, K.
  • Publisher: CRC Press
• [Book] Proteins and peptides as antioxidants, In : Bioactive Food Proteins and Peptides : Applications in Human Health (2012)
  • Author(s): Kim Se Kwon
  • Publisher: CRC Press