| Project/Area Number |
22700768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Notre Dame Seishin University |
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Principal Investigator |
SHIRAGA TOSHIYUKI ノートルダム清心女子大学, 人間生活学部, 准教授 (70363596)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | PEPT1 / 炎症性腸疾患 / 大腸炎ラット / Caco-2細胞 / サイトカイン / LPS / 酸化ストレス / ペプチド / 大腸炎誘発ラット / 血液指標 / 栄養状態 / NF-κB / Caco-2 / 単層培養 / 基底膜 / 大腸炎 / 炎症性サイトカイン |
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| Research Abstract |
In this study, the mechanism underlying the aberrant expression of the intestinal peptide transporter PEPT1 in inflammatory bowel disease was investigated using the human intestinal Caco-2 cell monolayer. PEPT1 expression was regulated by TNF-alpha and IL-6 at the mRNA level. A significant reduction in the levels of PEPT1 was observed after lipopolysaccharide stimulation, suggesting that this might be attributed to increased degradation or reduced synthesis of the PEPT1 protein. Additionally, PEPT1 mRNA levels decreased under oxidative stress conditions and are thought to be involved in the decrease in cell-cell adhesion. The effects of diet on the plasma index of the colitis rats were studied, but no peptide diet-specific influence was observed.
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