| Project/Area Number |
22700772
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Nagasaki International University |
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Principal Investigator |
NODAKE Yuich 長崎国際大学, 薬学部・生化学研究室, 助教 (30332282)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAKIBARA Ryuzo 長崎国際大学, 薬学部・生化学研究室, 教授 (30127229)
FUKASAWA Masashi 長崎国際大学, 薬学部・生化学研究室, 准教授 (20238439)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 薬学 / 食品 / 蛋白質 / 生理活性 / グレリン / グレリン受容体 / 摂食亢進ホルモン / 肥満予防 / 生活習慣病 / ホルモン-受容体相互作用 / 機能性食品 |
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| Research Abstract |
The binding mechanism of ghrelin that is a hunger-stimulating hormone to its receptor(GHS-R) has many unknown aspects. In this study, I tried to construct a comprehensive evaluation system of the interaction between both molecules. GHS-R cDNA was obtained from RT-PCR using rat stomach total RNA. Four extracellular regions of GHS-R that were important for binding to ghrelin were amplified by PCR based on the GHS-R cDNA and inserted into the expression vector. The interaction between recombinant extracellular region proteins fixed on a sensor chip and ghrelin was evaluated by surface plasmon resonance analysis.
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