Establishment of human induced cancer stem cells(iCSCs) and malignant brain tumor models derived from neural stem cells
| Project/Area Number |
22700865
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Carcinogenesis
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | NSCs / Brain tumor / mouse tumor model / ALK / グリオブラストーマ(GBM) / 神経幹細胞(NSC) / 活性型ALK / 発がんマウスモデル / 活性型H-RAS / 活性型EGFR |
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| Research Abstract |
Neural Stem Cells(NSCs) having self-renewal ability and multipotency are considered as one of the cells-of-origin of Brain tumors. The NSCs transduced with constitutively activated ALK rapidly formed highly proliferative and invasive brain tumors. Histological features of these tumors are similar to those of human glioblastoma maltiforme(GBM) such as necrosis, perivascular cuffing and giant cell formation. Immunohistochemistry analysis revealed that these tumors are positive for glioma markers and proliferation marker. These models are useful for investigating molecular mechanisms of gliomagenesis.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells2012
Author(s)
Tamada M, Nagano O, Tateyama S, Ohmura M, Yae T, Ishimoto T, Sugihara E, Onishi N, Yamamoto T, Yanagawa H, Suematsu M, Saya H
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Journal Title
Cancer Res
Volume: 72
Pages: 1438-48
Related Report
Peer Reviewed
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[Journal Article] Establishment of a choriocarcinoma model from immortalized normal extravillous trophoblast cells transduced with HRASV122011
Author(s)
Kobayashi Y, Shimizu T, Naoe H, Ueki A, Ishizawa J, Chiyoda T, Onishi N, Sugihara E, Nagano O, Banno K, Kuninaka S, Aoki D, Saya H
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Journal Title
Am J Pathol
Volume: 179
Pages: 1471-82
Related Report
Peer Reviewed
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[Journal Article] Transient depletion of p53 followed by transduction of c-Myc and K-Ras converts ovarian stem-like cells into tumor-initiating cells2011
Author(s)
Motohara T, Masuko S, Ishimoto T, Yae T, Onishi N, Muraguchi T, Hirao A, Matsuzaki Y, Tashiro H, Katabuchi H, Saya H, Nagano O
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Journal Title
Carcinogenesis
Volume: 32
Pages: 1597-606
Related Report
Peer Reviewed
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[Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells2011
Author(s)
Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka S, Akahata M, Kai K, Sugimoto H, Hirao A, Saya H
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Journal Title
Neoplasia
Volume: 13
Pages: 784-91
Related Report
Peer Reviewed
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[Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor2011
Author(s)
Sugihara E, Shimizu T, Kojima K, Onishi N, Kai K, Ishizawa J, Nagata K, Hashimoto N, Honda H, Kanno M, Miwa M, Okada S, Andreeff M and Saya H
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Journal Title
Oncogene
Volume: 31
Pages: 2849-61
Related Report
Peer Reviewed
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