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Establishment of human induced cancer stem cells(iCSCs) and malignant brain tumor models derived from neural stem cells

Research Project

Project/Area Number 22700865
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Carcinogenesis
Research InstitutionKeio University

Principal Investigator

ONISHI Nobuyuki  慶應義塾大学, 医学部, 特任助教 (40534540)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsNSCs / Brain tumor / mouse tumor model / ALK / グリオブラストーマ(GBM) / 神経幹細胞(NSC) / 活性型ALK / 発がんマウスモデル / 活性型H-RAS / 活性型EGFR
Research Abstract

Neural Stem Cells(NSCs) having self-renewal ability and multipotency are considered as one of the cells-of-origin of Brain tumors. The NSCs transduced with constitutively activated ALK rapidly formed highly proliferative and invasive brain tumors. Histological features of these tumors are similar to those of human glioblastoma maltiforme(GBM) such as necrosis, perivascular cuffing and giant cell formation. Immunohistochemistry analysis revealed that these tumors are positive for glioma markers and proliferation marker. These models are useful for investigating molecular mechanisms of gliomagenesis.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (19 results)

All 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells2012

    • Author(s)
      Tamada M, Nagano O, Tateyama S, Ohmura M, Yae T, Ishimoto T, Sugihara E, Onishi N, Yamamoto T, Yanagawa H, Suematsu M, Saya H
    • Journal Title

      Cancer Res

      Volume: 72 Pages: 1438-48

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells2012

    • Author(s)
      Mayumi Tamada, et al
    • Journal Title

      Cancer Research

      Volume: 72 Issue: 6 Pages: 1438-1448

    • DOI

      10.1158/0008-5472.can-11-3024

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fibroblast Growth Factor-2 Is an Important Factor that Maintains Cellular Immaturity and Contributes to Aggressiveness of Osteosarcoma2012

    • Author(s)
      Shimizu T., et al
    • Journal Title

      Mol Cancer Res

      Volume: 10(3) Issue: 3 Pages: 454-468

    • DOI

      10.1158/1541-7786.mcr-11-0347

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor2012

    • Author(s)
      Sugihara E, Saya H, et al
    • Journal Title

      Oncogene

      Volume: (印刷中) Issue: 23 Pages: 2849-2861

    • DOI

      10.1038/onc.2011.462

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a choriocarcinoma model from immortalized normal extravillous trophoblast cells transduced with HRASV122011

    • Author(s)
      Kobayashi Y, Shimizu T, Naoe H, Ueki A, Ishizawa J, Chiyoda T, Onishi N, Sugihara E, Nagano O, Banno K, Kuninaka S, Aoki D, Saya H
    • Journal Title

      Am J Pathol

      Volume: 179 Pages: 1471-82

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transient depletion of p53 followed by transduction of c-Myc and K-Ras converts ovarian stem-like cells into tumor-initiating cells2011

    • Author(s)
      Motohara T, Masuko S, Ishimoto T, Yae T, Onishi N, Muraguchi T, Hirao A, Matsuzaki Y, Tashiro H, Katabuchi H, Saya H, Nagano O
    • Journal Title

      Carcinogenesis

      Volume: 32 Pages: 1597-606

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells2011

    • Author(s)
      Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka S, Akahata M, Kai K, Sugimoto H, Hirao A, Saya H
    • Journal Title

      Neoplasia

      Volume: 13 Pages: 784-91

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor2011

    • Author(s)
      Sugihara E, Shimizu T, Kojima K, Onishi N, Kai K, Ishizawa J, Nagata K, Hashimoto N, Honda H, Kanno M, Miwa M, Okada S, Andreeff M and Saya H
    • Journal Title

      Oncogene

      Volume: 31 Pages: 2849-61

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 骨肉腫病態形成におけるFgf2の役割2011

    • Author(s)
      清水孝恒
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 誘導脳腫瘍幹細胞を用いた放射線抵抗性獲得モデルの確立2011

    • Author(s)
      大須賀覚
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 神経幹細胞を用いたマウス脳腫瘍モデルの構築2011

    • Author(s)
      大西伸幸
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 骨肉腫病態形成におけるFgf2の役割2011

    • Author(s)
      清水孝恒
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 誘導脳腫瘍幹細胞を用いた放射線抵抗性獲得モデルの確立2011

    • Author(s)
      大須賀覚
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 誘神経幹細胞を用いたマウス脳腫瘍モデルの構築2011

    • Author(s)
      大西伸幸
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] グリオーマ幹細胞モデルを用いた薬剤抵抗性克服2011

    • Author(s)
      Oltea Sampetrean
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-03
    • Related Report
      2011 Final Research Report
  • [Presentation] Igf2bp3/Imp3の異常発現は骨肉腫悪性度を決定する2011

    • Author(s)
      植木有紗
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-03
    • Related Report
      2011 Final Research Report
  • [Presentation] Igf2bp3/Imp3の異常発現は骨肉腫悪性度を決定する2011

    • Author(s)
      植木有紗
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] グリオーマ幹細胞モデルを用いた薬剤抵抗性克服2011

    • Author(s)
      Oltea Sampetrean
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://genereg.jp/index.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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