| Project/Area Number |
22700868
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 乳癌 / 糖代謝 / 3次元培養 / シグナル経路 / ヘキソサミン合成経路 / O-GlcNAc修飾 / Warburg効果 / シグナル伝達 |
|---|
| Research Abstract |
In this study, using three dimensional culture of breast cancer cells, the principal investigator examined mechanisms by which increased glucose metabolism regulates signaling pathways in cancer cells. In particular, the PI focused on hexosamine biosynsethis pathway(HBP), which branches from glycolytic pathway, and found that the enzymes in HBP play fundamental roles in signaling activity and malignant phenotype in cancer cells. Moreover, O-GlcNAc modification of proteins, which is downstream of HBP, and the enzyme responsible for O-GlcNAc modification were also found to be essential. Target proteins modified with O-GlcNAc specifically in cancer cells were also identified, and the roles of the proteins and the modification are now under investigation.
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