The investigation of function of repressor Bach1 in microenvironment of the tumor and the application to a mouse model for cancer stem cell transplantation.

• Project/Area Number: 22700869
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Tumor biology
• Research Institution: Tohoku University
• Principal Investigator: MATSUMOTO Mitsuyo 東北大学, 東北メディカル・メガバンク機構, 助教 (80400448)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: BACH1 / がん微小環 / Bach1 / がん微小環境

Research Abstract

We planned the construction of a Bach1^<-/-> immune-deficient mouse as a good recipient mouse model for engraftment and metastatic of human transplantable tumor cells. Therefore, we aimed to investigate the mechanism and role of Bach1 in fibroblast cell. To identify novel target genes of Bach1 in fibroblast cell, we performed ChIP-seq, DNA microarray, qPCR and western blotting analysis. These results suggest that Bach1 acts as a repressor of Pparγgene transcription in immortalized MEF (iMEF). Bach1^<-/-> iMEF more easily differentiated to adipocytes when treated with insuline, IBMX, dexamethasone and Pparγ ligand in vitro. Bach1 might play a role in adipose differentiation of fibroblast cell by modulating the actions of Pparγ.

Research Products

• [Journal Article] Methionine Adenosyltransferase II-dependent Histone H3K9 Methylation at the COX-2 Gene Locus (2013)
  • Author(s): Kera Y, Katoh Y, Ohta M, Matsumoto M, Takano-Yamamoto T, Igarashi K
  • Journal Title: J Biol Chem Volume: 288 Pages: 13592-13601
  • Peer Reviewed
• [Journal Article] Individual Transcriptional Activity of Estrogen Receptors in Primary Breast Cancer and Its Clinical Significance (2012)
  • Author(s): Gohno T, Seino Y, Hanamura T, Niwa T, Matsumoto M, Yaegashi N, Oba H, Kurosumi M, Takei H, Yamaguchi Y, Hayashi S
  • Journal Title: Cancer Medicine Volume: 1 Pages: 328-337
  • Peer Reviewed
• [Journal Article] Bach1 is critical for the transformation of mouse embryonic fibroblast by RasV12 and maintains ERK signaling (2012)
  • Author(s): Nakanome A, Brydoun A, Matsumoto M, Ota K, Funayama R, Nakayama K, Ono M, Shiga K, Kobayashi T, Igarashi K
  • Journal Title: Oncogene Volume: 32 Issue: 27 Pages: 3231-3245
  • DOI: 10.1038/onc.2012.336
  • Peer Reviewed
• [Presentation] Bach1の繊維芽細胞におけるPparg遺伝子発現制御を介した脂肪分化誘導 (2012)
  • Author(s): 松本光代、ブリドンアンドレイ、舟山 亮、西田有一郎、中山啓子、八重樫伸生、五十嵐和彦
  • Organizer: 第35回分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Bach1-mediated repression of Pparγ suppresses adipocyte differentiation of fibroblasts in vitro (2012)
  • Author(s): 松本 光代
  • Organizer: 第35回分子生物学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Bach1の転写制御標的遺伝子の同定 (2011)
  • Author(s): 松本光代、ブリドンアンドレイ、中目亜矢子、舟山亮、西田有一郎、中山啓子、八重樫伸生、五十嵐和彦
  • Organizer: 第34回分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] The Genome-Wide Identification of Direct Target Genes of Transcription Factor Bach1 (2011)
  • Author(s): 松本光代
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15