Screening for microRNA which is related to cancer progression by using novel microRNA inhibitory vectors
Project/Area Number |
22700873
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Tumor biology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | RNA干渉 / microRNA / inhibitor / がん / miRNA / miRNA inhibitor / miR-200 / EMT / E-cadherin / vimentin |
Research Abstract |
The purpose of this project is improvement of miRNA inhibitory vector "TuD RNA ; Tough Decoy RNA" which we developed previously and search miRNAs that are related to cancer progression by using improved TuD RNA vector. In this project, we improved TuD RNA expression vector. We transduced improved TuD RNA expression lentivirus vector into cancer cells and observed that its miRNA inhibitory effects has been maintained for more than 100 days after transduction. Furthermore, improved-type TuD RNA expression lentivirus vector which targeted miR-200c induced epithelial-mesenchymal transition in cancer cells. These results showed that improved-type TuD RNA was a powerful tool for miRNA research.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] A potent 2'-O-methylated RNA-based microRNA inhibitor with unique secondary structures2012
Author(s)
Haraguchi,T., Nakano, H., Tagawa, T., Ohki, T., Ueno, Y., Yoshida, T., Iba, H.
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Journal Title
Nucleic Acids Res.
Volume: (in press)
Issue: 8
Pages: 1-13
DOI
Related Report
Peer Reviewed
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[Journal Article] Micro RNAs miR-199a-5p and-3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers2011
Author(s)
Sakurai, K, Furukawa C, Haraguchi T, Inada K, Shiogama K, Tagawa T, Fujita S, Ueno Y, Ogata A, Ito M, Tsutsumi Y, Iba H
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Journal Title
Cancer Research
Volume: 71
Pages: 1680-1689
Related Report
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[Journal Article] MicroRNAs miR-199a-5p and -3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers.2011
Author(s)
Sakurai, K, Furukawa C, Haraguchi T, Inada K, Shiogama K, Tagawa T, Fujita S, Ueno Y, Ogata A, Ito M, Tsutsumi Y, Iba H.
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Journal Title
Cancer Research
Volume: 71
Pages: 1680-1689
Related Report
Peer Reviewed
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[Journal Article] Requiem Protein Links RelB/p52 and the Brm-type SWI/SNF Complex in a Noncanonical NF-kappa B Pathway.2010
Author(s)
Tando T, Ishizaka A, Watanabe H, Ito T, Iida S, Haraguchi T, Mizutani T, Izumi T, Isobe T, Akiyama T, Inoue J, Iba H.
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Journal Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume: 285
Pages: 21951-21960
Related Report
Peer Reviewed
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