| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
The purpose of this project is improvement of miRNA inhibitory vector "TuD RNA ; Tough Decoy RNA" which we developed previously and search miRNAs that are related to cancer progression by using improved TuD RNA vector. In this project, we improved TuD RNA expression vector. We transduced improved TuD RNA expression lentivirus vector into cancer cells and observed that its miRNA inhibitory effects has been maintained for more than 100 days after transduction. Furthermore, improved-type TuD RNA expression lentivirus vector which targeted miR-200c induced epithelial-mesenchymal transition in cancer cells. These results showed that improved-type TuD RNA was a powerful tool for miRNA research.
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