Genome-wide exploration of molecular targets for invasion of malignant pleural mesothelioma cell

• Project/Area Number: 22700882
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Tumor biology
• Research Institution: The University of Tokushima
• Principal Investigator: KAKIUCHI Soji 徳島大学, 病院, 講師 (50380100)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 悪性胸膜中皮腫 / 浸潤 / マイクロアレイ

Research Abstract

Genome wide gene expression analysis was performed to compare the human malignant mesothelioma(MPM) cells and their derived cells which had higher invasive capacity. Among the dozens of candidate genes, we focused on gene X, which was commonly down-regulated among highly invasive cells. By knocking down of gene X, invasiveness was accelerated, but proliferation and migration activities were not changed in parental cells. In orthotopic implantation model of MPM, the survival was statistically significantly shorter in gene X knock down group than scramble group, suggesting that gene X might be a target gene for a new treatment against the invasion of MPM.

Research Products

• [Journal Article] Epidermal growth factor receptor-tyrosine kinase inhibitor(gefitinib) augments pneumonitis, but attenuates lung fibrosis in response to radiation injury in rats (2012)
  • Author(s): Miyake K, Tani K, Kakiuchi S, Suzuka C, Toyoda Y, Kishi J, Tezuka T, Yuasa S, Hanibuchi M, Aono Y, Nishioka Y, Sone S
  • Journal Title: J Med Invest Volume: 59(1-2) Pages: 174-85
  • NAID: 130004465262
  • Peer Reviewed
• [Journal Article] Identification of genes potentially involved in bone metastasis by genome-wide gene expression profile nalysis of non-small cell lung cancer in mic (2012)
  • Author(s): Dat LT, Matsuo T, Yoshimaru T, Kakiuchi S, Goto H, Hanibuchi M, Kuramoto T, Nishioka Y, Sone S, Katagiri T
  • Journal Title: Int J Oncol Volume: 40 Pages: 1455-1469
  • DOI: 10.3892/ijo.2012.1348
  • Peer Reviewed
• [Journal Article] Erlotinib prevents experimental metastases of human small cell lung cancer cells with no epidermal growth factor receptor expression (2012)
  • Author(s): Gabr AG, Goto H, Hanibuchi M, Ogawa H, Kuramoto T, Suzuki M, Saijo A, Kakiuchi S, Trung VT, Sakaguchi S, Moriya Y, Sone S, Nishioka Y
  • Journal Title: Clin Exp Metastasis Volume: 29(3) Pages: 207-16
  • Peer Reviewed
• [Journal Article] Epidermal growth factor receptor-tyrosine kinase inhibitor (gefitinib) augments pneumonitis, but attenuates lung fibrosis in response to radiation injury in rats (2012)
  • Author(s): Miyake K, et al
  • Journal Title: J Med Invest Volume: 59 Pages: 174-85
  • Peer Reviewed
• [Journal Article] Erlotinib prevents experimental metastases of human small cell lung cancer cells with no epidermal growth factor receptor expression (2012)
  • Author(s): Gabr AG, et al
  • Journal Title: Clin Exp Metastasis Volume: 29 Pages: 207-16
  • Peer Reviewed
• [Journal Article] Investigation of the outpatient chemotherapy for lung cancer patients in Tokushima University Hospital (2011)
  • Author(s): Goto H, Hanibuchi M, Sakaguchi S, Kanematsu T, Kakiuchi S, Tomimoto H, Azuma M, Tezuka T, Tada H, Miki Y, Nakamura T, Sone S, Nishioka Y
  • Journal Title: J Med Invest Volume: 58(3-4) Pages: 219-26
  • NAID: 130004822660
  • Peer Reviewed
• [Journal Article] E7080 suppresses hematogenous multiple organ metastases of lung cancer cells with nonmutated epidermal growth factor receptor (2011)
  • Author(s): Ogino H, Hanibuchi M, Kakiuchi S, Trung VT, Goto H, Ikuta K, Yamada T, Uehara H, Tsuruoka A, Uenaka T, Wang W, Li Q, Takeuchi S, Yano S, Nishioka Y, Sone S
  • Journal Title: Mol Cancer Ther Volume: 10(7) Pages: 1218-28
  • Peer Reviewed
• [Journal Article] 5-Aminolevulinic acid-induced fluorescence diagnosis of pleural malignant tumor (2011)
  • Author(s): Ali AH, Takizawa H, Kondo K, Matsuoka H, Toba H, Nakagawa Y, Kenzaki K, Sakiyama S, Kakiuchi S, Sekido Y, Sone S, Tangoku A
  • Journal Title: Lung Cancer Volume: 74(1) Pages: 48-54
  • Peer Reviewed
• [Journal Article] Blockade of Th1 chemokine receptors ameliorates pulmonary granulomatosis in mice (2011)
  • Author(s): Kishi J, Nishioka Y, Kuwahara T, Kakiuchi S, Azuma M, Aono Y, Makino H, Kinoshita K, Kishi M, Batmunkh R, Uehara H, Izumi K, Sone S
  • Journal Title: Eur Respir J Volume: 38(2) Pages: 415-24
  • Peer Reviewed
• [Journal Article] The therapeutic efficacy of S-1 against orthotopically implanted human pleural mesothelioma cells in severe combined immunodeficient mice (2011)
  • Author(s): Van TT, Hanibuchi M, Kakiuchi S, Sato S, Kuramoto T, Goto H, Mitsuhashi A, Nishioka Y, Akiyama S, Sone S
  • Journal Title: Cancer Chemother Pharmacol Volume: 68(2) Pages: 497-504
  • Peer Reviewed
• [Journal Article] E7080 suppresses hematogenous multiple organ metastases of lung cancer cells with nonmutated epidermal growth factor receptor (2011)
  • Author(s): Ogino H, et al
  • Journal Title: Mol Cancer Ther Volume: 10 Pages: 1218-28
  • Peer Reviewed
• [Journal Article] Blockade of Th1 chemokine receptors ameliorates pulmonary granulomatosis in mice (2011)
  • Author(s): Kishi J, et al
  • Journal Title: Eur Respir J Volume: 38 Pages: 415-24
  • Peer Reviewed
• [Journal Article] The therapeutic efficacy of S-1 against orthotopically implanted human pleural mesothelioma cells in severe combined immunodeficient mice (2011)
  • Author(s): Van TT, et al
  • Journal Title: Cancer Chemother Pharmacol Volume: 68 Pages: 497-504
  • Peer Reviewed
• [Journal Article] 5-Aminolevulinic acid-induced fluorescence diagnosis of pleural malignant tumor. (2011)
  • Author(s): Ali AH, et al
  • Journal Title: Lung Cancer Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] The therapeutic efficacy of S-1 against orthotopically implanted human pleural mesothelioma cells in severe combined immunodeficient mice. (2010)
  • Author(s): Van TT, et al
  • Journal Title: Cancer Chemother Pharmacol Volume: (Epub ahead of print)
  • Peer Reviewed
• [Presentation] E7080, a multi-tyrosine kinase inhibitor suppresses the progression of malignant pleural mesothelioma with different proangiogenic cytokine production profiles. (2010)
  • Author(s): 柿内聡司
  • Organizer: 日本呼吸器学会総会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2010-04-23